Bacteria isolated from bengal cat (Felis catus×Prionailurus bengalensis) anal sac secretions produce volatile compounds associated with animal signaling

Anal sacs are an important odor producing organ found across the mammalian Order Carnivora. Secretions from the anal sac may be used as chemical signals by animals for behaviors ranging from defense to species recognition to signaling reproductive status. In addition, a recent study suggests that domestic cats utilize short-chain free fatty acids in anal sac secretions for individual recognition. The fermentation hypothesis is the idea that symbiotic microorganisms living in association with animals contribute to odor profiles used in chemical communication and that variation in these chemical signals reflects variation in the microbial community. Here we examine the fermentation hypothesis by characterizing volatile organic compounds (VOC) and bacteria isolated from anal sac secretions collected from a male bengal cat, a cross between a domestic cat and wild leopard cat (Felis catus×Prionailurus bengalensis).Both left and right anal sacs of a male bengal cat were manually expressed (emptied) and collected. Half of the material was used to culture bacteria or to extract bacterial DNA and other half was used for VOC analysis. DNA was extracted from the anal sac secretions and used for a 16S rRNA gene sequence based characterization of the microbial community. Additionally, some of the material was plated out in order to isolate bacterial colonies. The same three taxa,Bacteroides fragilis,Tessaracoccus, andFinegoldia magnawere abundant in the 16S rRNA gene sequence data and also isolated by culturing. Using Solid Phase Microextraction (SPME) gas chromatography-mass spectrometry (GC-MS), we tentatively identified 52 compounds from bengal cat anal sac secretions and 67 compounds from cultures of the three bacterial isolates chosen for further analysis.. Among 67 compounds tentatively identified from bacteria isolates, 52 were also found in the anal sac secretion.We show that the bacterial community in the anal sac consists primarily of only a few abundant taxa and that isolates of these taxa produce numerous volatiles that are found in the combined anal sac volatile profile. Many of these volatiles are found in anal sac secretions from other carnivorans, and are also associated with known bacterial biosynthesis pathways. This supports the fermentation hypothesis and the idea that the anal sac is maintained at least in part to house bacteria that produce volatiles for the host.

MORFOLOGI KELENJAR ANAL MUSANG LUAK BETINA (PARADOXURUS HERMAPHRODITUS)

The common palm civet (Paradoxurus hermaphroditus) is one of the mammalian in Indonesia which have anal gland in female and fefemale that located in the lower tail. This research was aimed to describe the morphology of anal gland of common palm civet female. One female of common palm civet was used in this research which observed macroscopic and microscopic.The result showed that common palm civet have a pair of anal gland which hasshaped like ball and located in the anal sacs wall’s in the ventrolateral of anus.Each anal gland has a ostium of anal canal dorsolaterally in the cutaneous zone of anal canal . Microscopic observation showed a well developed sebaceous gland and apocrine sweat gland in the wall of anal sac. Sebaceous and apocrine glands were excreted their product into main ductus to the anal canal.

Spinosity, regeneration, and targeting among Paleozoic crinoids and their predators

Evolving interactions between predators and prey constitute one of the major adaptive influences on marine animals during the Paleozoic. Crinoids and fish constitute a predator–prey system that may date back to at least the Silurian, as suggested by patterns of crinoid regeneration and spinosity in concert with changes in the predatory fauna. Here we present data on the frequency of breakage and regeneration in the spines of the Middle Devonian camerateGennaeocrinusand late Paleozoic cladids, as well as an expanded survey of the prevalence of spinosity and infestation by platyceratid gastropods on crinoid genera during the Paleozoic. Spine regeneration frequency in the measured populations is comparable to arm regeneration frequencies from MississippianRhodocrinitesand from modern deep-water crinoid populations. The prevalence of spinosity varies by taxon, time, and anatomy among Paleozoic crinoids; notably, spinosity in camerates increased from the Silurian through the Mississippian and decreased sharply during the Pennsylvanian, whereas spines were uncommon in cladids until their Late Mississippian diversification. Among camerates, tegmen spinosity is positively correlated with the presence of infesting platyceratid gastropods. These results allow us to evaluate several hypotheses for the effects of predation on morphological differences between early, middle, and late Paleozoic crinoid faunas. Our data corroborate the hypothesis that predators targeted epibionts on camerate crinoids and anal sacs on advanced cladids and suggest that the replacement of shearing predators by crushing predators after the Hangenberg extinction affected the locations of spines in Mississippian camerates.

Form and function of the musteloids

The musteloids are the most speciose of the carnivorans and have a global distribution. They display a wide diversity of morphological and physiological form and function, which have been shaped by their adaptation to a wide variety of ecological niches, ranging from the Arctic to the tropics and deserts to the seas. This chapter explores how several morphological and physiological adaptations are key to their successful diversification, including an elongated body, a highly insulating pelage, powerful teeth and jaws, anal sacs for olfactory communication or chemical defence, and reproductive physiologies that allow females to optimise their reproductive output. While many of these adaptations are shared by other carnivorans, it is their combination in musteloids that has allowed them to diversify so successfully globally.

Occurrence of Malassezia Yeasts in Dermatologically Diseased Dogs

The Malassezia genus is represented by several lipophilic yeasts, normally present on the skin of many warm-blooded vertebrates, including humans. The aim of this study was to investigate the occurrence of Malassezia yeasts in dogs with skin lesions (dermatitis, interdigital dermatitis and inflammation of anal sacs) and otitis externa. The presence of Malassezia spp. was investigated in a group of 300 dogs exhibiting clinical manifestations. The isolates of Malassezia were identified by using phenotypic (biochemical-physiological and morphological characteristics) and genotypic methods (PCR, RFLP-AluI, BanI and MspA1I) which allowed their precise identification. Malassezia yeasts were isolated from 84 specimens obtained from 76 positive dogs. M. pachydermatis was the most frequently isolated species (79 isolates) in this study. M. furfur was identified in four dogs and M. nana in one dog. The prevalence of isolated Malassezia spp. was 25.3 % in dogs with skin lesions; from which 36.0 % were dogs suffering from otitis externa, 24.5 % from dogs having dermatitis, 16.4 % from dogs with interdigital dermatitis and 14.3 % from dogs having inflammation of the anal sacs. A higher prevalence of Malassezia spp. was observed in animals with pendulous ears in comparison with dogs having erect ears.

Draft Genome Sequence of Enterococcus faecalis Strain UCD-PD3

Here, we present the draft genome sequence of Enterococcus faecalis strain UCD-PD3. The assembly contains 2,861,314 bp in 73 contigs. This strain was isolated from a feral domestic cat ( Felis catus ) anal sac secretion sample, as part of a project on isolating and characterizing the microbes present in feline anal sacs.

Clinical studies on anal tumors in dogs

The anal and perianal region contains multiple structure and glands, some of which are unique to the area. Anal sacs or perianal sinuses are paired, lateral, cutaneous, anal diverticula lined by a cornified, stratified squamous epithelium. Dogs have apocrine tubular anal sac glands that open into the anal sacs. Perianal glands are modified sebaceous glands, they are referred as hepatoid glands, because of their histologic resemblance to hepatocytes. Incidence,symptoms and surgical and medical management of anal tumors was studied.