Integrating active learning activities and metacognition into STEM writing courses

While active learning is highly recognized and recommended in the educational community, instructors are still struggling with how to incorporate active learning tools into writing courses. In this article, we have 1) described specific challenges that we have encountered in the course of Molecular Cell Biology Laboratory-Critical Thinking through Writing (BIOL3810-CTW); 2) introduced the active learning approaches and metacognition integrated into this writing-intensive course; 3) demonstrated the effectiveness of these active learning approaches, and 4) shared the principles of integrating active learning activities into writing courses in science, technology, engineering, and mathematics (STEM) and beyond.

First person – Agathe Chaigne

ABSTRACT First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Agathe Chaigne is first author on ‘ Three-dimensional geometry controls division symmetry in stem cell colonies’, published in JCS. Agathe is a postdoc in the lab of Ewa Paluch at the MRC Laboratory for Molecular Cell Biology (LMCB), University College London, London, UK, investigating the crosstalk between cell division and cell fate transitions during development.

First person – Anna Dowbaj

ABSTRACT First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Anna Dowbaj is co-first author on ‘ An optogenetic method for interrogating YAP1 and TAZ nuclear–cytoplasmic shuttling’, published in JCS. Anna conducted the work described in this article while a PhD student in the lab of Erik Sahai at the Francis Crick Institute, London, UK. She is now a postdoc in the lab of Meritxell Huch at the Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, investigating the role of the stem cell niche in liver regeneration.

Cell scientists to watch – Zdeněk Lánský and Marcus Braun

ABSTRACT Zdeněk Lánský received his PhD in physics from the Charles University in Prague in 2006. He then moved to The Netherlands for his postdoc, where he studied the biophysics of molecular motors – first at the Vrije Universiteit in Amsterdam with Erwin Peterman, and then at Wageningen University with Marcel Janson. In 2011 he joined the lab of Stefan Diez at the B CUBE Center for Molecular Bioengineering in Dresden, where his research focused on crosslinker-mediated force generation in the cytoskeleton. Zdeněk set up his lab at the Institute of Biotechnology, Czech Academy of Sciences, BIOCEV in 2015, which he has run together with Marcus Braun since 2017. Marcus Braun received his PhD in biochemistry from King's College London in 2009 for his work on microtubule-based molecular motors in the labs of Andrew McAinsh and Rob Cross. He then moved to Dresden for his postdoc to study cytoskeletal transport, with a particular focus on microtubule crosslinkers, in Stefan Diez's lab – first at the Max Planck Institute of Molecular Cell Biology and Genetics, then later at B CUBE. In 2017, he joined Zdeněk Lánský at the Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, as a principal investigator. The Lánský and Braun lab investigates ensemble dynamics of cytoskeletal proteins.

Uveitis: Molecular Pathogenesis and Emerging Therapies

The profound impact that vision loss has on human activities and quality of life necessitates understanding the etiology of potentially blinding diseases and their clinical management. The unique anatomic features of the eye and its sequestration from peripheral immune system also provides a framework for studying other diseases in immune privileged sites and validating basic immunological principles. Thus, early studies of intraocular inflammatory diseases (uveitis) were at the forefront of research on organ transplantation. These studies laid the groundwork for foundational discoveries on how immune system distinguishes self from non-self and established current concepts of acquired immune tolerance and autoimmunity. Our charge in this review is to examine how advances in molecular cell biology and immunology over the past 3 decades have contributed to the understanding of mechanisms that underlie immunopathogenesis of uveitis. Particular emphasis is on how advances in biotechnology have been leveraged in developing biologics and cell-based immunotherapies for uveitis and other neuroinflammatory diseases.

CellPAINT: Turnkey Illustration of Molecular Cell Biology

CellPAINT is an interactive digital tool that allows non-expert users to create illustrations of the molecular structure of cells and viruses. We present a new release with several key enhancements, including the ability to generate custom ingredients from structure information in the Protein Data Bank, and interaction, grouping, and locking functions that streamline the creation of assemblies and illustration of large, complex scenes. An example of CellPAINT as a tool for hypothesis generation in the interpretation of cryoelectron tomograms is presented. CellPAINT is freely available at http://ccsb.scripps.edu/cellpaint.

Versatile CRISPR/Cas9 Systems for Genome Editing in Ustilago maydis

The phytopathogenic smut fungus Ustilago maydis is a versatile model organism to study plant pathology, fungal genetics, and molecular cell biology. Here, we report several strategies to manipulate the genome of U. maydis by the CRISPR/Cas9 technology. These include targeted gene deletion via homologous recombination of short double-stranded oligonucleotides, introduction of point mutations, heterologous complementation at the genomic locus, and endogenous N-terminal tagging with the fluorescent protein mCherry. All applications are independent of a permanent selectable marker and only require transient expression of the endonuclease Cas9hf and sgRNA. The techniques presented here are likely to accelerate research in the U. maydis community but can also act as a template for genome editing in other important fungi.

First person – Sarita Hebbar and Malte Lehmann

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Sarita Hebbar and Malte Lehmann are co-first authors on ‘Mutations in the splicing regulator Prp31 lead to retinal degeneration in Drosophila’, published in BiO. Sarita is a post-doctoral fellow in the lab of Elisabeth Knust at the Max Planck Institute of Molecular Cell Biology & Genetics, investigating how the same metabolic pathways regulate temporally distinct processes (in morphogenesis and later in tissue homoestasis). Malte is a post-doctoral researcher and physician in the lab of R. G. Kühl and A. G. Siegmund at Charité, Universitätsmedizin Berlin, investigating the mechanisms behind inflammation in Inflammatory Bowel Disease patients.

First person – Srija Bhagavatula

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Srija Bhagavatula is first author on ‘A putative stem-loop structure in Drosophila crumbs is required for mRNA localisation in epithelia and germline cells’, published in JCS. Srija is a post-doc in the lab of Dr Elisabeth Knust at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, investigating the significance of mRNA localization in epithelia.