Genome-Wide Association Analysis of Salt-Tolerant Traits in Terrestrial Cotton at Seedling Stage

Soil salinization is the main abiotic stress factor affecting agricultural production worldwide, and salt stress has a significant impact on plant growth and development. Cotton is one of the most salt-tolerant crops. Therefore, the selection and utilization of salt-tolerant germplasm resources and the excavation of salt resistance genes play important roles in improving cotton production in saline–alkali soils. In this study, we analysed the population structure and genetic diversity of a total 149 cotton plant materials including 137 elite Gossypium hirsutum cultivar accessions collected from China and 12 elite Gossypium hirsutum cultivar accessions collected from around the world. Illumina Cotton SNP 70 K was used to obtain genome-wide single-nucleotide polymorphism (SNP) data for 149 elite Gossypium hirsutum cultivar accessions, and 18,430 highly consistent SNP loci were obtained by filtering. It was assessed by using PCA principal component analysis so that the 149 elite Gossypium hirsutum cultivar accessions could be divided into two subgroups, including subgroup 1 with 78 materials and subgroup 2 with 71 materials. Using the obtained SNP and other marker genotype test results, under salt stress, the salt tolerance traits 3d Germination potential, 3d Radicle length drop rate, 7d Germination rate, 7d Radicle length drop rate, 7d Germination weight, 3d Radicle length, 7d Radicle length, Relative Germination potential, Relative Germination rate, 7d Radicle weight drop rate, Salt tolerance index 3d Germination potential index, 3d Radicle length index, 7d Radicle length index, 7d Radicle weight index and 7d Germination rate index were evaluated by GWAS (genome-wide association analysis). A total of 27 SNP markers closely related to the salt tolerance traits and 15 SNP markers closely related to the salt tolerance index were detected. At the SNP locus associated with phenotyping, Gh_D01G0943, Gh_D01G0945, Gh_A01G0906, Gh_A01G0908, Gh_D08G1308 and Gh_D08G1309 related to plant salt tolerance were detected, and they were found to be involved in intracellular transport, sucrose synthesis, osmotic pressure balance, transmembrane transport, N-glycosylation, auxin response and cell amplification. This study provides a theoretical basis for the selection and breeding of salt-tolerant upland cotton varieties.

Monitoring of the state of electromagnetic fuel injectors of gasoline engines with distributed fuel injection systems

Introduction. Ensuring the trouble-free operation of road transport is one of the priority tasks. The functioning of the internal combustion engine, the most important unit, is impossible without the correct operation of the fuel injectors. A modern electromagnetic nozzle is a fairly reliable element of the engine, however, it can quickly fail when using low-quality fuel. Constant monitoring of the technical condition of the injectors with the help of builtin vehicle diagnostics systems will prevent such negative consequences as fuel overspending, the formation of harmful substances in the exhaust gases, deterioration of the traction and speed characteristics of the vehicle.Materials and methods. The research was carried out by the method of physical modelling, a laboratory stand was developed that simulates the operation of the engine fuel system. As an object, the Bosch 0280 158 996 nozzle was selected, which was controlled using an Arduino-based electronic unit. A series of one-factor experiments was carried out, according to the results of which the dependence of the pressure drop rate on the contamination of the nozzle was constructed.Results. The permissible standard of the pressure drop rate during the operation of the electromagnetic nozzle is determined, which allows unambiguously determining its technical condition (serviceable/not serviceable). An algorithm for checking injectors is proposed, as well as a method for its implementation on modern passenger cars.Discussion and conclusion. The obtained dependence qualitatively coincides with the results of studies by other authors. There is a successful experience of implementing such algorithms on modern cars. The implementation of the research results will allow timely detection of faulty (contaminated) electromagnetic injectors and prevent negative effects on both engine parts and the environment.

A New Rock Brittleness Evaluation Method Based on the Complete Stress-Strain Curve

Brittleness is a crucial parameter of rock mass and the key indicator in rock engineerings, such as rockburst prediction, tunnelling machine borehole drilling, and hydraulic fracturing. To solve the problem of using present brittleness indexes, the existing rock brittleness indexes were firstly summarised in this paper. Then, a brittleness index (BL), which considers the ratio of stress drop rate and stress increase rate and the peak stress, was proposed. This new index has the advantage of simplifying the acquisition of key parameters and avoiding dimensional problems, as well as taking the complete stress-strain curves into account. While applying the BL, the peak strain is used to describe the difficulty of brittle failure before the peak point, and the ratio of stress drop to strain increase can reflect the stress drop rate without dimension problem. In order to verify the applicability of BL, through the PFC2D, the microparameters and confining pressure were changed to model different types of rock numerical specimens and different stress condition. The results show that the BL can well reflect and classify the brittleness characteristics of different rock types and characterise the constraint of confining pressure on rock brittleness. Moreover, the influence of microparameter on macroparameter was studied. In order to further verify the reliability of the brittleness index (BL), this study conducted uniaxial and triaxial compression tests (30 MPa) on marble, sandstone, limestone, and granite under different confining pressure.

Studies on the Effects of NH3 in H2 and Air on the Performance of PEMFC

The effect of NH3 in H2 and in air was investigated at various concentrations ranging from 1.0 ppm to 100 ppm in air and ranging from 0.25 ppm to 10 ppm in fuel. The effect of NH3 on cathode caused an instantaneous decrease in cell voltage which dropped from 0.734 V to 0.712 V in 30 h and drop rates was 0.73 mV/h for 1 ppm; however, the cell voltage dropped to 0.415 V in 1 h for 100 ppm of NH3. The voltage could not be recovered after the polarization test (V-I test) but could be recovered to 84.4% after operation with neat air for 1.5 h and 98.4% after cycle voltammogram (CV). It was found that the voltage drop was obvious, and the drop rate increased with the NH3 concentration in H2. The voltage drop rates at 500 mA/cm2 were 0.54 mV/h for 0.5 ppm of NH3, 0.8 mV/h for 1 ppm, and 2 mV/h for 10 ppm. The voltage could be recovered from 70.6% to 77.3% after discharged with high purity H2 for 24 h, to 92.8% after being purged with clean air for 10 h and to 98.4% after CV scan. The tolerance concentration of NH3 in H2 for 1000 h was 40 ppb, for 2000 h was 20 ppb, and for 5000 h was 9 ppb.

Numerical Study on Effects of Pipeline Geometric Parameters on Release Characteristics of Gas Extinguishing Agent

In order to guide the optimization design of the pipeline network of the aircraft-fixed gas fire extinguishing system and improve its fire extinguishing performance, FLUENT software was used to simulate the influence of pipeline parameters such as diameter, length, and roughness on the release characteristics of the fire extinguishing agent. It can be found that the extinguishing agent can be divided into liquid and vapor extinguishing agents in the fire extinguishing pipeline system during the release. The spatial distribution and proportion of the liquid and vapor extinguishing agents are asymmetric. Results show that the peak value of the pressure drop rate (dPmax) has a good quadratic function relationship with the pipeline diameter (D) and the functional relationship is dPmax=−22.224+2.782D+0.089D2, which means that the peak value increased significantly with the increase in the pipeline diameter. Moreover, when the pipeline diameter is 25 mm, the average pressure drop rate of the vessel is about 35.02 MPa/s, which is 5.97 times the value of the average pressure drop rate when the pipeline diameter is 10 mm. With the increase in the pipeline diameter, the release time decreases significantly, the mass flow rate increases obviously, while the gasification ratio decreases rapidly at first and then increases slightly. The pipeline length also has a significant influence on the release characteristics of the extinguishing agent. With the increase in the pipeline length, the release time and the gasification ratio increase linearly, while the mass flow rate decreases linearly. Compared with the pipeline diameter and pipeline length, the influence of the pipeline roughness on the release characteristics of the extinguishing agent is weak. With the increase in the pipeline roughness, the release time and the gasification ratio of the extinguishing agent increases slowly, while the mass flow rate decreases slowly.

Experimental Study on Impermeability Law of Aquiclude Reconstructed by Mudstone of External Dump in Arid Zone

In order to achieve the goal of ecological restoration, plant irrigation, and water retention in the external dump of open-pit coal mine in the arid desert area, it is proposed to use the mudstone in the stripped material to reconstruct the surface aquiclude and improve the water holding capacity of the topsoil. By taking the Hongshaquan Open-Pit Coal Mine as the study object, the red mudstone of mining level +650 was selected as the topsoil aquiclude material through the geological survey. XRD diffraction experiments are used to determine the composition of red mudstone including kaolinite mineral, quartz, potash feldspar, albite, and illite. The moisture content of the red mudstone is 4.16% as measured by the indoor drying experiment. And the particle size of 0.5 mm, 1 mm, and 2 mm and the thickness of 5 cm, 10 cm, and 20 cm rock samples were selected to conduct the indoor soil column experiment. Three indicators of initial infiltration rate, stable infiltration rate, and average infiltration rate were obtained according to the analysis and calculation of the water column drop rate, wet peak drop rate, and cumulative infiltration rate so that the permeability law and coefficient of each group of tests can be known. The finite element transient analysis theory and numerical simulation method were adopted to verify the results of physical experiments. The research results show that +650 level red mudstone has a strong antiseepage ability, and the smaller the particle size, the better the antiseepage performance. For 0.5 mm and 2 mm particle sizes, as the paving height increases, the water-proof effect is better. The laying height of 1 mm particle size has no obvious influence on the water barrier effect. The physical simulation was confirmed to have the same result trend as the numerical simulation, both of which are quadratic functions with the error within a reasonable range.

DG-LoRa: Deterministic Group Acknowledgment Transmissions in LoRa Networks for Industrial IoT Applications

In this paper, we propose a novel MAC protocol, called DG-LoRa, for improving scalability in low power wide area networks. DG-LoRa is backward compatible with legacy LoRaWAN and adds new features, such as group acknowledgment transmissions in the time-synchronized frame structure that supports determinism on channel access. In DG-LoRa, the number of responses to data frames that are transmitted from end devices is maximized by allocating the spreading factor and timeslot in the frame structure. We evaluate the performance of DG-LoRa using the Monte-Carlo simulation and then compare it with the performance of legacy LoRaWAN in terms of data drop rate and the number of retransmissions. Our numerical results show that DG-LoRa supports approximately five times more connections to the LoRa network satisfying a 5% data drop rate. Also, it is observed that DG-LoRa enables low overhead by reducing the number of data frame retransmissions.