COMPARATIVE DATA ON MACROZOOBENTHOS COMMUNITY BETWEEN EASTERN AND WESTERN COASTS OF SAZANI ISLAND, ALBANIA

This paper is focused on analysing the difference in distribution, composition and abundance of benthic invertebrates' populations of the rocky coast of Sazan Island (south western Albania), between its eastern and western sides. This study was carried out during 2012-2014, sampling in shallow water. A total of 56 species of benthic macroinvertebrates were recorded. Species composition and their abundance were compared between the four sampling sites and between the two sampling seasons, spring and autumn. Degree of coast exposure and level of environmental impacts seem to be the main factors regarding the difference in species composition and quantitative characteristics of macrozoobenthic community between the eastern and western coasts of the island.

Phyto-ecological studies and distribution pattern of plant species and communities of Dhirkot, Azad Jammu and Kashmir, Pakistan

Plant species represent the hierarchical expression of vegetation as it is affected by various environmental gradients. We explored the plant species composition, distribution pattern, communities formation and their respective indicators under the influence of various environmental factors in the Dhirkot region, Azad Jammu and Kashmir. It was hypothesized that different environmental factors were responsible for the formation of various plant communities each with a distinct indicator. Quantitative ecological techniques were used for the sampling of vegetation. A total of 114 quadrats were established in 13 selected sampling sites. Phytosociological attributes were calculated for each plant species at each quadrat. Soil samples were collected and analyzed using different standard protocols. All the collected data were analyzed using Cluster Analysis, Indicator Species Analysis and Canonical Correspondence Analysis of PCORD and CANOCO software, respectively. A total of 145 plant species were recorded belong to 62 different families. Asteraceae and Lamiaceae were the dominant families, represented by 12 species each (8.27%). Cluster Analysis classify all the stations and plants into four major plant communities as 1) Olea-Desmodium-Prunilla community. 2) Abies-Zanthoxylum-Pteracanthus community 3) Cedrus-Elaeagnus-Hypericum community 4) Alnus-Myrsine–Ranunculus community. Soil pH, electrical conductivity, soil saturation, organic matter and altitude were the significant environmental factors that play its essential role in the plant species distribution, composition, formation of major plant communities and their respective indicators in the region. It is recommended that the identified indicator and rare plant species of the investigated area can further be grown for conservation and management purposes in in-situ environment.

COLLECTIVE MONOGRAPH «SOILS OF THE LVIV REGION»: THEORETICAL AND PRACTICAL SIGNIFICANCE

The peer-reviewed collective monograph “Soils of the Lviv region” reflects longterm studies of the genetic nature, geographical distribution, composition andproperties of the main types and subtypes of soils in the Lviv region. The monograph contains: a preface, 10 chapters, a list of used literature, an application, information about the authors. The text is illustrated with tables, figures, modern photographs of soil profiles. The total volume of the monograph is 424 pages. This is the first theoretical and practical scientific work, which contains full information on the state of soils and soil cover in the Lviv region. Until now, the main source of information about the soils of the region were the materials of a large-scale soil survey carried out in 1957–1961 years and consecrated in the publications of Y. Olenchuk andA. Nikolin “The soils of Lviv region”, which was published in 1969. The peerreviewed monograph is a fundamental scientific work in the field of soil research, problems of modern lands use and protections.

Sustainability of Wetlands in the Eyes of the New Generation of Environmental Engineering Students

This research paper investigates the general understanding and skills of young third-year environmental engineering students concerning the criteria for monitoring wetlands. Students were asked to describe approaches, data, and final solutions to identify and/or eliminate wetland odor. Over half of the students (55%) did not consider the “identification” strategy by which to determine the source of unexpected odors. The rest gave answers focused on “restoration” (24%) and “prevention” (15%) strategies, while 6% of the answers were unrelated. In fact, without knowing the causes and without thinking about the necessity/importance of visiting/surveying the site, students came up with a priori solutions. In their mind, the immediate cause of the odor could be a disturbed or unbalanced distribution/composition of ions, salts, or microbes in the water. Indeed, most data types (71%) were those related to “water”. As the final solution, 50% of responses considered “physical” followed by “legal and planning” solutions (32%). Each factor, including “Chemical” and “Biological”, constituted 9% of the answers. This research highlights engineering students’ communication skills, critical thinking and problem-solving abilities by contemplating class activities that simulate real-world situations. The research presumes that these engineering student cohorts represent typical student communities worldwide. Such findings contribute to policymaking in the engineering education system on a larger scale.

Effect of replacing surface with underground rubbish containers on urban House Sparrows Passer domesticus

Urbanisation processes are increasing worldwide at surprising rates affecting wildlife in many ways: changing habitat structure, reducing resources, and modifying the distribution, composition and abundance of local biota. In different countries, urban waste collection techniques are evolving and surface rubbish containers (neighbourhood receptacles for temporarily storing anthropogenic household waste located above-ground on the streets) are being replaced with underground ones (metal boxes with steel chutes that fed into large underground containers) to improve sanitation measures, to avoid bad smells and waste scattering by animals. We aimed to detect if House Sparrows were more abundant close to surface rubbish containers than close to the underground ones. We recorded an abundance index of House Sparrows during two visits in winter 2018–2019 to point counts located in groups of both container types (80 and 85 groups of underground and surface containers, respectively) in eight towns of Eastern Spain. We modelled the abundance index according to rubbish container type, and 14 other environmental variables at four scales: container, nearest buildings, near urban features, and general locality features using GLMMs. House Sparrows were more abundant close to surface than to underground rubbish containers, which may be linked with higher food debris availability. The presence of other urban features (bar terraces, private gardens, mature trees) interacting with the rubbish containers also influenced the abundance of House Sparrows. The replacement of above-ground rubbish containers with underground ones may deprive House Sparrows resources, which could lead to the decline of this species, especially in urban areas with little green cover.

A Large-Scale Sequencing-Based Survey of Plasmids in Listeria monocytogenes Reveals Global Dissemination of Plasmids

The food-borne pathogen Listeria monocytogenes is known for its capacity to cope with multiple stress conditions occurring in food and food production environments (FPEs). Plasmids can provide benefits to their host strains, and it is known that various Listeria strains contain plasmids. However, the current understanding of plasmid frequency and function in L. monocytogenes strains remains rather limited. To determine the presence of plasmids among L. monocytogenes strains and their potential contribution to stress survival, a comprehensive dataset was established based on 1,921 published genomes from strains representing 14 L. monocytogenes sequence types (STs). Our results show that an average of 54% of all L. monocytogenes strains in the dataset contained a putative plasmid. The presence of plasmids was highly variable between different STs. While some STs, such as ST1, ST2, and ST4, contained few plasmid-bearing strains (<15% of the strains per ST), other STs, such as ST121, ST5, ST8, ST3, and ST204, possessed a higher proportion of plasmid-bearing strains with plasmids found in >71% of the strains within each ST. Overall, the sizes of plasmids analyzed in this study ranged from 4 to 170 kbp with a median plasmid size of 61 kbp. We also identified two novel groups of putative Listeria plasmids based on the amino acid sequences of the plasmid replication protein, RepA. We show that highly conserved plasmids are shared among Listeria strains which have been isolated from around the world over the last few decades. To investigate the potential roles of plasmids, nine genes related to stress-response were selected for an assessment of their abundance and conservation among L. monocytogenes plasmids. The results demonstrated that these plasmid genes exhibited high sequence conservation but that their presence in plasmids was highly variable. Additionally, we identified a novel transposon, Tn7075, predicted to be involved in mercury-resistance. Here, we provide the largest plasmid survey of L. monocytogenes to date with a comprehensive examination of the distribution of plasmids among L. monocytogenes strains. Our results significantly increase our knowledge about the distribution, composition, and conservation of L. monocytogenes plasmids and suggest that plasmids are likely important for the survival of L. monocytogenes in food and FPEs.

Glycosaminoglycans: Carriers and Targets for Tailored Anti-Cancer Therapy

The tumor microenvironment (TME) is composed of cancerous, non-cancerous, stromal, and immune cells that are surrounded by the components of the extracellular matrix (ECM). Glycosaminoglycans (GAGs), natural biomacromolecules, essential ECM, and cell membrane components are extensively altered in cancer tissues. During disease progression, the GAG fine structure changes in a manner associated with disease evolution. Thus, changes in the GAG sulfation pattern are immediately correlated to malignant transformation. Their molecular weight, distribution, composition, and fine modifications, including sulfation, exhibit distinct alterations during cancer development. GAGs and GAG-based molecules, due to their unique properties, are suggested as promising effectors for anticancer therapy. Considering their participation in tumorigenesis, their utilization in drug development has been the focus of both industry and academic research efforts. These efforts have been developing in two main directions; (i) utilizing GAGs as targets of therapeutic strategies and (ii) employing GAGs specificity and excellent physicochemical properties for targeted delivery of cancer therapeutics. This review will comprehensively discuss recent developments and the broad potential of GAG utilization for cancer therapy.