ABSENCE OF OXIDATIVE STRESS AND SIRTUINS RECRUITMENT ON CARDIAC TISSUE POST STRESS

Stress has emerged as a factor associated with cardiovascular disease. Catecholamines released during the stress reaction by the sympathetic nerves and the adrenal medulla couple to β1-and β2-adrenoceptors in the cardiomyocytes membrane enhancing heart function in order to attend the organism demand. This might produce excessive reactive oxygen species what may culminate with oxidative stress and progression of several cardiac diseases. Sirtuins have been described as cardioprotective factors and important regulators of the cellular stress response in the heart. The aim of this work is to investigate the putative participation of oxidative stress and sirtuins in the heart of rats submitted to foot shock stress, an experimental model where there is up regulation of β2-adrenoceptors and downregulation of β1-adrenoceptors. The data have shown that in the myocardium of rats submitted to foot shock stress the H2O2 concentration, catalase and superoxide dismutase activity, NAD+/NADH ratio, as well as the protein expression of sirtuins 1 and 3 were not altered. Pharmacological blockade of the β2-adrenoceptors by ICI118,551, did not modify this scenario. It is concluded that foot shock stress does not cause disruptions in oxidative stress or redox state processes in the myocardium, and consequently, sirtuins are not recruited to stress response.

Personified program for correction of post-stress disorders in different categories of survivors (combatants and volunteers)

Combat stress has a significant impact on the individual. It is a major cause of combat mental trauma and stress disorders development. According to the World Health Organization, combat mental trauma is a direct cause of stress disorders in 80 % of survivors of hostilities, and in its long term are often observed: maladaptation, autoaggressive behavior, anxiety and depression, addiction. Against the background of use of the developed program, positive dynamics of mental state, decrease in levels of anxiety and depression, decrease in the level of groups of PTSD symptoms on the Impact of Event Scale were noted. Reducing level of maladaptation according to the method of K. Rogers and R. Diamond and level of social frustration. Thus, the developed personalized program for the correction of post-stress disorders contributes to the productive dynamics of psychopathological symptoms, reducing emotional tension, restoring a sense of psychological well-being, creating a new cognitive model of life.

The journey of Deinococcus radiodurans; a perspective

: Deinococcus radiodurans has been recognized for its robustness and recorded in the Guinness Book of World Records as the world's toughest known bacterium. In essence, the title comes from its ability to survive extreme conditions such as severe drought (desiccation) and radiation tolerance up to 15000 Gy, which is more than 250 times of E. coli and about 3000 times of humans. Due to its high tolerance to all kinds of genotoxic stress, such as desiccation, UV, X-rays, and oxidants, D. radiodurans is a well-suited model organism for microbial radiation resistance studies. The DNA damage-responsive gene expression is an important component of post-stress recovery where the cell shows a great multiplicity of genomes leading to the highly proficient recombinational DNA repair. This article pitches light on the unique properties of D. radiodurans, unfolding its journey so far as well as important molecular discoveries, prospects, and biotechnological applications.

FUZZY TAKAGI-SUGENO-KANG FOR MENTAL HEALTH CONSULTATION RECOMMENDATIONS

The condition of students experiencing trauma makes consultation a necessity, there is no mental health examination that can be accessed by students, parents, psychologists, student affairs using the fuzzy Takagi-Sugeno-Kang method so it is necessary to be able to create a recommendation system for student mental health consultations by using fuzzy Takagi-Sugeno-Kang. In accordance with the case given, each student with a neurosis score of 8, psychoactive substances 11, psychotic 10 and post stress trauma disorder 13 had a mental health symptom level of 24.28, meaning they were at a moderate level. With a mental health recommendation system, it can be handled earlier so that learning activities can run according to the desired achievements.

Mortality among patients undergoing dipyridamole 99mTc Sestamibi SPECT: impact of blunted heart rate response and abnormal scans

Background Blunted heart rate response (BHRR) caused by cardiac neuropathy associated with dipyridamole stress, has been linked to cardiovascular (CV) outcome events. Whether BHRR is necessarily associated with abnormal perfusion is unknown. The aim of the study was to assess the incremental prognostic value of BHRR in a single center population undergoing Dipyridamole 99mTc Sestamibi SPECT test (DSPECT) for predicting late CV events. Methods 388 patients (aged 73±10 years, 45% females, 51% with known coronary disease) that underwent DSPECT over 3 years period were included. Abnormal DSPECT and BHRR were evaluated in relation to late death. Results Mean follow up period was 1560±565 (15–2431) days. During follow up period, 90 patients died. Mode of death was CV in 20 and non-CV in 70. BHRR (<20% heart rate increase), abnormal DSPECT, post-stress LVEF <60% and reversible defects were observed in 63%, 41%, 23% and 20% of patients, respectively. BHRR (HR -2.41, p<0.0006) and abnormal DSPECT (HR-1.62, p=0.02) were predictors of all-cause death. BHRR had incremental prognostic value over abnormal DSPECT (Figure, p<0.0005). Multivariable analysis identified age, dyspnea, insulin treated diabetes mellitus and dialysis as independent predictors of death while DSPECT and BHRR did not. However, BHRR remained a significant predictor of CV death [HR 8.1 (1.06, 62.0), p<0.05]. Conclusions In this contemporary DSPECT cohort, BHRR and DSPECT failed to predict all-cause mortality. However, BHRR was an independent predictor of CV death. FUNDunding Acknowledgement Type of funding sources: None. BHRR stratifies abnormal DSPECT

Effects of Garlic Allium sativum Powder on Nutrient Digestibility, Haematology, and Immune and Stress Responses in Eurasian Perch Perca fluviatilis Juveniles

The supplementation of fish diets with phytogenics can increase growth performance and can modulate immune system response. European perch Perca fluviatilis (initial weight 25.0 ± 0.4 g) were fed a diet including 0 (Control), 10 (G10), 20 (G20), and 30 (G30) g kg−1 garlic powder. No significant difference in the growth parameters and somatic indices were observed. Significantly higher fat digestibility was observed in G10 and G30 diets compared to in the control and G20 diets(p < 0.05). Significantly greater red blood cell and white blood cell counts were observed with the G10 diet (p < 0.05). Garlic significantly decreased serum cholesterol in all of the experimental groups. Serum albumin was significantly higher in the G10 and G20 diets (p < 0.05). Immediately after the overcrowding stress challenge, the garlic groups showed significantly higher cortisol levels than the control group, while no significant difference was observed in the glucose concentration among groups. At 1 h post-stress, all of the groups that had been fed a garlic-supplemented diet showed lower cortisol levels than the control group, and this trend was maintained at 6 and 24 h post stress (p < 0.05), and glucose level in all garlic groups was significantly lower than control (p < 0.05). Garlic at 10 g kg−1 in feed can improve apparent fat digestibility and selected blood parameters and can enhance resistance against high-density and net handling stress in Eurasian perch.

Antioxidant Potential of the Bio-Based Fucose-Rich Polysaccharide FucoPol Supports Its Use in Oxidative Stress-Inducing Systems

Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by exogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by Enterobacter A47, containing ca. 17 wt% of negatively charged residues in its structure. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe3+-TPTZ species at an EC50 of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% w/v FucoPol concentration shown previously to be cryo- and photoprotective was also demonstrated to protect Vero cells against H2O2-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality.

A Historical Reconstruction of Some Pronominal Suffixes in Modern Dialectal Arabic

The morphology of the pronominal suffixes in dialectal Arabic are of particular interest for scholars of the history of Arabic for two main reasons. First, multiple dialects attest suffixes that, from a comparative perspective, apparently retain final short vowels. The second and more complicated issue concerns the vowels which precede the suffixes in the dialects, which are thought to either have been case inflecting or epenthetic. In this paper, I take up Jean Cantineau’s “embarrassing question” of how to account for the development of the vowels of the pronominal suffixes. Based on data from dialectal tanwīn in modern dialects, and attestations from pre-modern texts as well, I will argue that the pre-suffix vowels did originate in case inflecting vowels, but that no historical model heretofore proposed can satisfactorily account for how the various dialectal forms might have arisen. I identify two major historical developments and propose models for each. First, I suggest that dialects in which the pre-suffixal vowels harmonized with the suffix vowels developed via a process of harmonization across morpheme boundaries before the loss of final short vowels. For dialects in which one vowel is generalized, I argue that a post-stress neutralization took place, which led to a single vowel both before suffixes and tanwīn as well. Finally, I rely on evidence from the behavior of the suffixes to argue that the final vowel of the 3fs suffix was originally long, but that those of the 3ms, 2ms, and 2fs were most likely short.