in vitro assembly
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2021 ◽  
Author(s):  
Sofia Lövestam ◽  
Fujiet Adrian Koh ◽  
Bart van Knippenberg ◽  
Abhay Kotecha ◽  
Alexey G Murzin ◽  
...  

Abundant filamentous inclusions of tau are characteristic of more than 20 neurodegenerative diseases that are collectively termed tauopathies. Electron cryo-microscopy (cryo-EM) structures of tau amyloid filaments from human brain revealed that different tau folds characterise many different diseases. A lack of laboratory-based model systems to generate these structures has hampered efforts to uncover the molecular mechanisms that underlie tauopathies. Here, we report in vitro assembly conditions with recombinant tau that replicate the structures of filaments from both Alzheimer's disease (AD) and chronic traumatic encephalopathy (CTE), as determined by cryo-EM. Our results suggest that post-translational modifications of tau modulate filament assembly, and that previously observed additional densities in AD and CTE filaments may arise from the presence of inorganic salts, like phosphates and sodium chloride. In vitro assembly of tau into disease-relevant filaments will facilitate studies to determine their roles in different diseases, as well as the development of compounds that specifically bind to these structures or prevent their formation.


Viruses ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1853
Author(s):  
Martin Obr ◽  
Florian K. M. Schur ◽  
Robert A. Dick

The small cellular molecule inositol hexakisphosphate (IP6) has been known for ~20 years to promote the in vitro assembly of HIV-1 into immature virus-like particles. However, the molecular details underlying this effect have been determined only recently, with the identification of the IP6 binding site in the immature Gag lattice. IP6 also promotes formation of the mature capsid protein (CA) lattice via a second IP6 binding site, and enhances core stability, creating a favorable environment for reverse transcription. IP6 also enhances assembly of other retroviruses, from both the Lentivirus and the Alpharetrovirus genera. These findings suggest that IP6 may have a conserved function throughout the family Retroviridae. Here, we discuss the different steps in the viral life cycle that are influenced by IP6, and describe in detail how IP6 interacts with the immature and mature lattices of different retroviruses.


Author(s):  
Corrado Grappiolo ◽  
Raimon Pruim ◽  
Matthias Faeth ◽  
Paolo de Heer
Keyword(s):  

2021 ◽  
Author(s):  
Kellie D. Licking-Murray ◽  
Darby J. Carlson ◽  
Ryan Sowle ◽  
Kimberly A. Carlson
Keyword(s):  

Author(s):  
Eijaz Ahmed Bhat ◽  
Nasreena Sajjad ◽  
Irfan A. Rather ◽  
Jamal S.M. Sabir ◽  
Yan-Yan Hor

Life ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 334
Author(s):  
Dinh To Le ◽  
Kristian M. Müller

Virus-like particles (VLPs) are increasingly used for vaccine development and drug delivery. Assembly of VLPs from purified monomers in a chemically defined reaction is advantageous compared to in vivo assembly, because it avoids encapsidation of host-derived components and enables loading with added cargoes. This review provides an overview of ex cella VLP production methods focusing on capsid protein production, factors that impact the in vitro assembly, and approaches to characterize in vitro VLPs. The uses of in vitro produced VLPs as vaccines and for therapeutic delivery are also reported.


Biomaterials ◽  
2021 ◽  
pp. 120808
Author(s):  
Jorge Santini-González ◽  
Jennifer A. Simonovich ◽  
Roberto Castro-Gutiérrez ◽  
Yarelis González-Vargas ◽  
Nicholas J. Abuid ◽  
...  

Author(s):  
Mabel Rodríguez ◽  
Ricardo M. Castro-Acosta ◽  
Elias R. Ruiz-Morales ◽  
Francisca Villanueva-Flores ◽  
Octavio T. Ramírez ◽  
...  

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Adam J. Wargacki ◽  
Tobias P. Wörner ◽  
Michiel van de Waterbeemd ◽  
Daniel Ellis ◽  
Albert J. R. Heck ◽  
...  

AbstractRecent advances in computational methods have enabled the predictive design of self-assembling protein nanomaterials with atomic-level accuracy. These design strategies focus exclusively on a single target structure, without consideration of the mechanism or dynamics of assembly. However, understanding the assembly process, and in particular its robustness to perturbation, will be critical for translating this class of materials into useful technologies. Here we investigate the assembly of two computationally designed, 120-subunit icosahedral complexes in detail using several complementary biochemical methods. We found that assembly of each material from its two constituent protein building blocks was highly cooperative and yielded exclusively complete, 120-subunit complexes except in one non-stoichiometric regime for one of the materials. Our results suggest that in vitro assembly provides a robust and controllable route for the manufacture of designed protein nanomaterials and confirm that cooperative assembly can be an intrinsic, rather than evolved, feature of hierarchically structured protein complexes.


Nucleus ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 82-89
Author(s):  
Kentaro Tamura ◽  
Haruko Ueda ◽  
Ikuko Hara-Nishimura

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