Abstract
Bone marrow (BM) chimeric mice are a valuable tool in the field of immunology and hematology, and genetic manipulation of donor cells is widely used to study gene function under physiological and pathological settings. Current BM chimera protocols generally require use of multicolor fluorescence-activated cell sorting (FACS) for donor hematopoietic stem and progenitor cell (HSPC) purification. Here, we describe a cell culture technique for the enrichment of functional HSPCs from mouse BM without the use of FACS purification. This technique is therefore expected to overcome current limitations in mouse BM chimera models.