Futsal is a sport on the rise worldwide, attracting more and more new practitioners and as well as in field soccer, it has undergone changes in recent years, increasingly demanding athletes, becoming a high impact sport, promoting overload, in the short, medium and long term, predisposing to injury to different degrees of the locomotor apparatus. The aim of this study was to analyze lower limbs asymmetries in futsal-based athletes, as well as their relationship to the incidence of injuries. The study was developed with 47 athletes of the basic category, futsal, from a city in the interior of the state of São Paulo, where functional tests were performed with the help of the PHAST application. A pattern of similarity was identified between the muscle groups tested, except for the gluteus muscle, presenting a significant difference. According to the indicators found, the patterns of strength deficit of the middle glutes may trigger, or predispose to, some types of biomechanical lesions whether proximal, in the hip region, or distal, as dysfunctions in the knee joint. When an athlete presents with a weakness of this muscle, ipsilateral femur adduction, increased medial rotation and fall of the pelvis against lateral, promoting increased dynamic angle of the knee, increasing the overload in this joint. Thus, it is concluded that pre-season evaluation is important to identify possible asymmetries, implementing preventive work to correct them, in order to minimize the risks of injuries.