The Arrangement of Corpus Geniculatum Laterale Connections with Oculomotor Structures

The primary visual centres are known to be involved in the organisation of oculomotor acts, but the pathways of signal transmission from corpus geniculatum laterale (lateral geniculate nucleus, LGN) to the structures of the oculomotor system remain unknown. The aim of this study on 30 cats was to determine autoradiographically all the possible pathways of visual information transmission from both dorsal and ventral nuclei of the LGN to oculomotor nuclei. It was found that there were no direct connections of the LGN with the oculomotor nucleus. The connection occurs either through the cortex or through the preoculomotor formations. These pathways are the following: (1) from the dorsal and ventral nuclei of the LGN to the visual pretectum (olivary pretectal nucleus, posterior pretectal nucleus, nucleus of the optic tract) and then to the vegetative part of III nucleus or through nucleus commissurae posterioris, Cajal and Darkschewitsch nuclei to the somatis part of III nucleus and along the medial longitudinal fasciculus to IV nucleus and periabducens region; (2) from the ventral LGN into the deep layers (IV and VI) of the superior colliculus, and then to the Edinger - Westphal nucleus, preoculomotor central gray substance, and VI nucleus; (3) from the dorsal LGN into the deep layers (IV and VI) of the superior colliculus with relay synapses in the parietal cortex and zona insecta; (4) from the dorsal and ventral nuclei of the LGN to nucleus pontis dorsolateralis and paramedianus, being connected with the vermis anterior lobe (V - VII lobes) of cerebellum, and then to nucleus vestibularis inferior and nucleus vestibularis lateralis.

Somatostatin-immunoreactive nerve cell bodies and fibers in the medulla oblongata et spinalis.

Complete serial sectioning of the medulla oblongata in monkey, cat, guinea pig, and japanese dancing mouse and incubation for somatostatin-immunoreaction was carried out. Numerous regions of the medulla oblongata such as the nucleus reticularis gigantocellularis, nucleus cuneatus et gracillis, nucleus raphe magnus, nucleus tractus solitarius, nucleus vestibularis, and parts of the oliva contain dense networks of somatostatin-immunoreactive nerve fibers. Cell bodies were seen in the nucleus reticularis medullae oblongatae. In the spinal cord the sections from each segment were analyzed, showing the highest concentrations of somatostatinergic fibers in the substantia gelantinosa of the columna dorsalis. Cell bodies were seen in the zona intermedia centralis, especially in the upper cervical segments. Many positive fibers were also seen in the entire zona intermedia and the columna ventralis. Especially prominent was the immunoreactivity in the zona intermediolateralis of the thoracic segments and the columna ventralis of the lower lumbar and sacral segments.

ULTRASTRUCTURE OF THE SPOON TYPE SYNAPTIC ENDINGS IN THE NUCLEUS VESTIBULARIS TANGENTIALIS OF THE CHICK

The fine structure of the "spoon" type synaptic endings of the chick tangential nucleus was studied with the electron microscope. These endings often measure ∼18 µ in length by ∼3–4 µ in width. The axoplasm of the endings contains very few synaptic vesicles, a large number of neurofilaments oriented parallel to the long axis of the nerve fiber, and microtubules and numerous mitochondria. The synaptic membrane complex shows areas of localized occlusion of the synaptic cleft with the formation of an external compound membrane. It has not been decided whether these areas have a disc shape; their length measures between 0.04 and 0.47 µ. The five-layer pattern characteristic of an external compound membrane is shown in specimens fixed with formalin—OsO4, glutaraldehyde—acrolein—OsO4, and acrolein KMnO4 but it does not appear in the glutaraldehyde-OsO4-fixed specimens. The over-all thickness of the external compound membrane varies depending upon the fixative used. The synaptic clefts in the regions between the external compound membrane discs are widened and measure ∼300 A. A condensation of dense material occurs in pre- and postsynaptic cytoplasms all along the synaptic membrane complex. The morphological relationships described in the spoon endings are suggestive of electrical transmission.