Retinal fundus photographs capture hemoglobin loss after blood donation

Recently it was shown that blood hemoglobin concentration could be predicted from retinal fundus photographs by deep learning models. However, it is unclear whether the models were quantifying current blood hemoglobin level, or estimating based on subjects' pretest probability of having anemia. Here, we conducted an observational study with 14 volunteers who donated blood at an on site blood drive held by the local blood center (ie, at which time approximately 10% of their blood was removed). When the deep learning model was applied to retinal fundus photographs taken before and after blood donation, it detected a decrease in blood hemoglobin concentration within each subject at 2-3 days after donation, suggesting that the model was quantifying subacute hemoglobin changes instead of predicting subjects' risk. Additional randomized or controlled studies can further validate this finding.

ASSESTMENT OF FEMALE REPRODUCTIVE FUNCTION IN PADDLEFISH (POLYODON SPATHULA) REARED IN POND CONDITIONS IN ASTRAKHAN REGION

The article describes the problems, which are of greatest interest for the formation of fish broodstock, such as the process of gameto- and gonadogenesis and duration of spawning cycles in broodstock reared in artificial conditions. Under the Research and Experimental Aquaculture Complex BIOS of CaspNIRKH in the formed paddlefish broodstock 63 females matured in 2020. There were studied 11 paddlefish females born in 2002 and 2003, whose roe was taken for food purposes. The following parameters were determined: age of sexual maturity, duration of the spawning interval, body weight, ratio of eggs to the fish body weight. The physiological state of paddlefish females upon obtaining roe was assessed by biochemical parameters of blood: hemoglobin concentration (Hb) and total serum protein (TSP). The parameters of the reproductive function of paddlefish females obtained in the research were lower than the parameters characteristic for paddlefish in their natural habitat, and lower than the parameters of the first acclimatizers in the Krasnodar region. Sexual maturity of the studied females came quite late - at the age of 14-15, which is 1-2 years more than in the conditions of the Krasnodar region. The spawning intervals of the studied paddlefish females turned out to be 1-2 years longer than in the natural conditions. The ratio of eggs to the female body weight was about 10%, which is 1.5 times less than that observed in the American paddlefish in the natural conditions. At the same time, the biochemical parameters of blood (hemoglobin concentration and total serum protein, which characterize the physiological state of paddlefish females) turned out to be rather high and indicated a satisfactory condition of the fish. The preliminary results obtained make it possible to recommend the fish farmers of the enterprise to improve the work with paddlefish broodstock, paying special attention to optimizing the conditions for fish rearing at the stages of gonad formation.

Hemodynamic index of animals with burning injury treated with “Acerbin”

Thermal injuries are wide spread among home pets and are represented as a recent problem of veterinary medicine because of the lack of anti-burn drugs adapted for use in veterinary medicine. In this regard, the aim of our work is to certify the vulnerary spray “Acerbin” for medical purposes in the treatment of thermal injuries of animals and to evaluate its therapeutic potency according to the dynamics of healing burn injuries and recovery of the blood cell composition. The research was carried out on 60 laboratory white mice. Thermal injuries of the skin integument on the dorsal surface of the croup were simulated; the “Acerbin” spray was further applied. To achieve the aim, the blood samples and planimetric measured tests of the area of burn defects were taken on the 3rd, 5th, 7th, 10th and 14th day of treatment. The samples were analyzed by the quantitative content of erythrocytes, thrombocytes, leucocytes and cHb (blood hemoglobin concentration) using an automatic hematological analyzer “Abacus vet 10”. Planimetric indicators of the area of burns were subjected to mathematical processing with the calculation of the rate of epithelization of the burn injury. The obtained hematological and planimetric digital indicators were processed by statistical methods of analysis, compared and then interpreted.

Prevalence of anemia in university going girls and its ratio between hostellites and day scholars.

Anemia is considered as a reduction in blood hemoglobin concentration. It is associated with micronutrient deficiency such as iron deficiency anemia. IDA has strong health implications on girls during reproductive age.