Influenza Persists

World Health Organization estimates that worldwide annually there are about one billion infections, 3-5 million severe illnesses, and 300,000-500,000 deaths (10.1038/s41572-018-0002-y). Influenza is caused primarily by influenza A and influenza B viruses. Influenza A is the cause of pandemics.

Investigation of Reactive Oxygen Species production in Human Hepatocytes

1. Aim/Background: Reactive oxygen species (ROS) have been identified as compounds responsible for producing cellular damage. The purpose of this research is to examine if there is production of reactive oxygen species through free radical intermediates within human hepatocytes treated with morphine, bilirubin, or furosemide. The investigation examines the early stages of biotransformation by measuring the levels of reactive oxygen species produced inside of the treated hepatocytes within the first and second hours of treatment. The experiment was designed upon a case of a jaundiced (elevated bilirubin) infant who received morphine and furosemide and later died through unknown mechanisms. The experiment looks to examine if these drug compounds could contribute to cellular damage. This can help to further understand the potential interactions and complications of free radical intermediates produced during the phases of biotransformation. 2. Method: Previously cultured human hepatocytes were washed by centrifugation and re-suspended in 1x supplemental buffer to a concentration of 1x106 cells/mL and seeded in a dark clear bottom 96-well microplate at 100,000 stained cells/well. The cells were treated with either furosemide, morphine, bilirubin, a Tert-Butyl hydro peroxide (TBHP) positive control, or left as a background. Reactive oxygen generated in the presence of these agents were quantified by fluorescence excitation/emission measurement at 495nm/529nm. Fluorescence was measured at one and two hours. ROS generated convert 2',7'-dichlorodihydrofluorescein diacetate to 2',7'-dichlorodihydrofluorescein within the cells, which fluoresces. The fluorescence intensity detected is equivalent to the level of ROS generated. Wells that were untreated were used as blanks and subtracted from background and TBPH. 3. Results: Furosemide and Morphine did not produce statistically significant levels of ROS (p >0.05) above the background in both hours 1 and 2 of biotransformation and ROS measurement (Figure 1). Although Bilirubin did not produce statistically significant (p >0.05) levels of ROS above the background (Figure 2) during the first hour, it did produce statistically significant levels in the second hour of biotransformation. Each compound’s level of ROS was reduced during the second hour, signaling the removal of intermediate ROS metabolites (Figure 2). The production of ROS in each compound signifies that there is biotransformation to an intermediate that produces ROS. 4. Conclusion: The production of ROS above the background by each of the compounds shows there is an intermediate free radical compound that is produced during the biotransformation of each compound [21]. In this study, although furosemide and morphine did not produce statistically significant levels of ROS in both hours of biotransformation, bilirubin did produce significant levels of ROS in the second hour of biotransformation. This finding is in line with previous studies that shows morphine to offer protective effects against ROS production [16, 17]; and bilirubin demonstrating deleterious production of ROS at high doses [18]. Further work must be done to examine the correlation between the levels of ROS and extent of hepatocellular damage.

Follow-up Strategies in Focal Liver Lesions and Treatment Methods

Today, advances in cross-sectional imaging have led to the detection and early recognition of incidental/focal liver lesions (FCL). In approximately 17,000 cases of chest CT, incidental liver lesions were found in 6% [1]. In general, FCL consists of hepatocytes, biliary epithelium, mesenchymal tissue, connective tissue, or metastasized cells from distant sites. Most incidental lesions are benign, some may require careful management and treatment. In evaluating the lesion, the patient's clinical history, underlying disease and age factor should be considered. FCL can be detected at a rate of 10-30% in normal healthy and chronic liver disease patients, and even in oncology patients with malignancy, FCLs can be highly benign (50-80%)

Relevance of Prevention of Postoperative Refluxes

Gastrectomy is a complex operation for stomach diseases. Despite the successes to date, there are still no cardinal grounds to recognize the results of gastrectomy as satisfactory

Causes of Duodeno-Gastric Reflux as a Surgical Problem

The author points to duodeno-gastric reflux as gastric precancer. In the overwhelming majority of patients, GHD is caused by compression of the lower-horizontal part of the duodenum by the superior mesenteric artery.

Analysis Results of Yarrow Medicinal Plant Used as Gastroprotective and Its Use in Health

Introduction: Herbal treatments are a sub-branch of traditional and complementary medicine. People generally use it to strengthen health. It is mostly used with the advice of radio-television, internet and friends. Among the side effects, the most frequent allergic reactions can be seen. tandardized products should be used.It is reported that 80% of the essential oils of mercury (Achillea herba L.) must be monoterpene. Sesquiterpene group is guaianolite, germacranolite, edesmanolite and has anti-inflammatory, anti-edematous, antimicrobial effect. As flavonoid routine, it contains apigenin. Quinine has effects that increase secretion and appetite. It is spasmolytic due to azulene and flavonoids. It is used in gastric mucosa irritations. It is defined as a gastroprotective drug. Azulene and camazulene give wound healing properties to essential oil. The symptoms of inflammation are relieving. Method: Plants (Achillea herba L.) Grown in July Organic Farm in Konya region were used. Analyzes were conducted in the laboratory of the Western Mediterranean Antalya Regional Directorate of Agriculture and Forestry. Total volatile oil content flavonoid amount was determined in the analysis. The amount of essential oil of lavender plant grown in Konya region was measured by hydrodistillation method. Essential oil components were measured by GC-MS / FID method. Result: In this study, we aimed to present phytochemical analysis of mercury (Achillea herba L.)) Plant grown in Konya region. Total essential oil amount was found to be 0.62%. Piperitone takes the first place with 31.25% and p-mentha -1, 5-dien-8-ol with 14.49% and α-pinene with 10.61%. When the content analysis of European countries is examined in the literature studies, it is seen that the amount of Kamuzulen and monoterpene in the plants grown in Estonia region is higher than other European countries (Hungary, Greece, Moldova, Lithuania, and Germany). Millefolii herba is recommended as gastroprotective drug. It is also used after prostate adenectomy because of its antiinflammatory properties.

Values of Obese Male Patıents Performed Wıth Acupuncture

Obesity is a disease defined by excessive fat storage in the body. It is an energy balance problem; the increase in body fat is caused by an imbalance between energy intake and energy expenditure. The practice of acupuncture views the body as a combination of the soul and the body, not merely as a physical structure. This interpretation can lead to a successful therapy for the treatment of chronic pain. One of the factors for its success is the concept of the functional evaluation of the organ. According to acupuncture, the organs are alive and vitality is provided by the energy of life called ‘’qi’’ Pathogens that interrupt and obstruct the flow of qi form diseases. Acupuncture needles are inserted at specific points on the Bonghan channels and the qi stream is regulated by electron transfer. The present study was a cross-sectional study conducted in 2019 in xxx University’s GETAT Center. Patients aged between 18–65 years with a BMI > 25 were included in the study. Acupuncture was shown to be effective not only in the obese groups but also in the control groups. Therefore, it could be beneficial in preventing weight gain. Both ear and body acupuncture points were used effectively in obesity patients. Therefore, acupuncture may be recommended as an effective adjunct in the treatment of obesity. Acupuncture can reduce the stress of patients on diets and increase patient compliance.

A Review of Colitis Ulcers in the Gastrointestinal Tract

Colitis ulcer disease is a chronic disorder that affects the gastrointestinal tract. The disease is characterized by abnormal inflammation of the inner surface of the rectum and intestine, which makes up most of the length of the large intestine. Inflammation usually causes open sores (ulcers) in the large intestine. Doctors still do not know exactly what causes ulcerative colitis. Ulcerative colitis is an inflammatory bowel disease, but genetics appear to play an important role. Family history is seen in many patients with ulcerative colitis. In fact, having a relative with ulcerative colitis is the biggest risk factor for the disease, meaning you may inherit the genetic cause of ulcerative colitis. In addition, some environmental factors are involved in the immune system attacking the colon and developing ulcerative colitis. Families with ulcerative colitis share some of the genes that appear to be linked to the disease. Note that several genetic changes may play a role in the development of ulcerative colitis. Genes may stimulate ulcerative colitis by altering the immune system response or disrupting the protective barrier of the intestinal wall.

Stomach This: A Novel Type of Gastritis

Atrophic gastritis can be environmental in origin and involve the antrum or autoimmune in origin and involve the body and fundus. We present a rare case of autoimmune atrophic pangastritis (AIAP), a distinct type of autoimmune gastritis (AIG) affecting the entire stomach, which should be considered in patients with other autoimmune disorders.

Radiologic Findings of Splenic Abscess: A Report of Two Cases

Splenic abscess is a very rare entity with an incidence of about 0.14-0.7% in autopsy studies. Splenic abscess has been reported to have a mortality rate of about47%, it has various causes but usually associated with trauma and infections of the spleen. We report two cases of splenic abscess; a child with post traumatic cause and a middle aged lady with an unknown cause. Both cases were diagnosed of having the abscess following imaging; the first case had abdominopelvic ultrasound that showed an abscess cavity with a volume of about 11mls and subsequently resolved following medical treatment with intravenous antimicrobial agents. The second case however had an abdominopelvic computed tomography scan that showed a huge splenic abscess with a volume of about 1400mls; she had a successful splenectomy. We report the radiologic findings of these cases due to the rare nature of splenic abscess as reported in the literature.