Development of a Universal Scale for Validating the Process of Sanitization of Equipment, Materials and Surfaces in the Animal Facility

Background: Monitoring of sanitization of cage equipment is an essential function of any laboratory animal facilities, seeking to ensure the animal health and welfare. The purpose of the current study was to develop universal scale for monitoring sanitization through detection of residual ATP for the most effective process of sanitizing components of rodents micro- and macro-environment. Methods: Sterile pens and swabs for the HY-LiTE® Luminometer Instrument were used to measure ATP concentration (RLU) on the cleaned surface samples. We have examined the elements of the microenvironment (rodent and rabbit cage, cage wire meshes, feed separating pieces, water bottles with tips, houses, tunnels, IVC cage frames, cage tops and cage wire meshes). The assessed swab area on each of the surfaces was 10×10 cm. Result: We observed, that combined washing (cleaned manually and with automatic universal washers with detergent) gives the lowest RLU values. Monitoring of the quality of sanitization of equipment and surfacescan be carried out without the use of microbiological tests. Use of pre-washing allows increasing the service life of caging equipment. We have developed an assessment scale to monitor sanitizing, which can be recommended to scientific and breeding animal facilities for monitoring sanitization cage equipments.

Certain issues of legal regulation of animal insurance as a compulsory form of insurance

Agricultural insurance is a prerequisite for the existence of agriculture, especially for agricultural countries. The present time poses serious climatic, economic, and biological challenges to agricultural production. Insurance should become a tool that will help to balance the risks between all participants in agricultural insurance relations. Insurance should reduce the negative impact on agricultural production and living standards in the country. In the article on the basis of analysis of normative-legal acts, regulating the procedure of agricultural animals insurance the problematic issues are investigated and the ways of elimination of collisions in legal regulation of this type of insurance are offered. The problems of determining the list of animals subject to compulsory insurance are investigated and solutions are proposed. The question of legal expansion of the range of insurance risks for insurance contracts of animals in the framework of the Law of Ukraine «On peculiarities of insurance of agricultural products with state support» is analyzed. The proposed additions to the regulatory and legal acts in the field of insurance of breeding animals will lead to the harmonization of relevant legislation, as well as improve awareness of breeding animals for regulatory authorities, consumers of relevant products and insurers. Keywords: insurance, compulsory animal insurance, breeding animal insurance, insurance risk, insurance of agricultural products with state support

Agchabadi District of The New Garadolag Village Administrative Area Earth Cover Study

It is known from the brief nature of the territory of the research district that the date of establishment of Agjabadi district - 08.08.1930 The territory - 1.76 thousand sq.m. km Population - 136.8 thousand people (January 1, 2020) Population density - 1 sq. km. km 78 people (January 1, 2020). Agjabedi district is located in Mil and Garabagh plains of Kur-Araz lowland. Agjabedi city and Sarvanlar, Kurds, Minakhorlu, Garavelli, Galabadin, Avshar, Muganli, Shahsevan, Koyuk, Tazakend, Khojavend, Salmanbeyli, Ashagi Avshar, Hindarkh, Imamgulubeyli, Mirzahagverdili, Sarijali, Balakhrizli, Taynaq, Poinag , Sharafkhanli, Shotlanli, Shenlik, Husulu, Ranjbarlar, Boyat, Hajilar, Garakhanli, Hajibadalli, Pariogullar, Agabeyli, Garadolag, Mehrabli, Kabirli, Aran, Yeni Garadolag, Najaf-gulubeyli, Qiyameddinli, Shahsevan-Tazakand, Shahsevan-Tazakand, Includes Jafarbeyli and Yukhari Qiyameddinli villages. The relief of the region is flat, gradually rising from the north-east to the south-west. The surface of the area is composed of continental-alluvial and marine sediments of the Anthropogenic system. There is a clay deposit. The climate is temperate hot, dry subtropical. The average temperature is 1.2-1.7 ° C in January and 25-26 ° C in July. Annual precipitation is 300-500 mm. The river network is sparse. The Kura River flows along the north-eastern border, and the Gargar River flows through the central part. The Upper Karabakh canal passes through the region. There is a salt lake in the area. Gray-meadow, gray, meadow-gray soils are widespread. In the central part, saline and saline soils are found. The plants are of steppe and semi-desert type. There are bushes and sparse Tugay forests on the banks of the Kura River. Animals: gazelle, wolf, wild boar, jackal, fox, swamp beaver, badger, gray rabbit, Asia Minor sand mouse, etc. Birds: turkey, pigeon, black grouse, pheasant, etc. Aggol National Park is located in Agjabadi district. 15 species of animals, 20 species of fish and 40 species of plants have been recorded here. Agjabadi is mainly an agricultural region. Fruit growing, cotton growing, grain growing, silkworm breeding, animal husbandry, etc. developed.

Zootechnical data analysis in a breeding animal facility: tracing the patterns of mouse production

Background With the enactment of the Brazilian Law Arouca 11,794/2008 and Decree 6.899/2009, there has been an urgent need for changes in the processes related to animal experimentation in Brazil; in particular, there is a need for improvements in enhancements of the lab animal management. To improve the management capacity of the Lab animal facility of the Carlos Chagas Institute’s Laboratory Animals Science (LAS), BioterC software was developed and implemented in 2014 for tracking mouse laboratory colonies. Five years after the implementation of this software, we sought to analyze the information in the database originated from BioterC using the Exploratory Analysis Data methodology (EDA). This article aims to identify animal breeding patterns using a data mining tool (Data Science) with Python programming language. Results The results show that from September 2014 to June 2019, under the license IACUC number LW- 6/17, 15.106 animals were produced. The C57BL/6, BALB/c and Swiss strains were the most frequently produced strains. The distribution of births due to crosses between these strains showed a median of 6 to 10 animals, depending on the genetic homozygosis and heterozygosis of the animal. The median number of days of mating was 35 days. In the sexing period, the records reported a median of 19 days. A total of 393 requests for animals from internal and external laboratories were registered. It was noted that approximately half of the animals produced to meet the demand for orders were discarded. Of the 15,106 animals, 38% were requested for animal experimentation, 58% were discarded and 4% did not have an outcome recorded in the data. Conclusions This volume of data provides an initial view of the information retrieval capabilities contained in BioterC, allowing for unique breeding knowledge by installing laboratory animals.

Influence of breeding pig breeding on efficiency production of the industry

The article considers the problems of development of breeding pig breeding in Ukraine as a basis for the functioning of efficient and competitive commodity production. According to the results of the study, with the reform of agricultural production, commodity pig farming suffered significant losses, which had a negative impact on the efficient operation of breeding farms, especially breeders. The main reasons for the intensification of destructive phenomena in the field of breeding pig farming are the unstable level of purchase prices for pig products, low profitability and unprofitable production. It is obvious that the organization of the domestic selection system has a significant impact on reducing the cost and increasing the profitability of pig production, ensuring the quality and competitiveness of breeding (genetic) resources. The results of scientific research show that a significant increase in productivity in pig breeding is achieved through the effect of heterosis in interbreeding, and it is proved that under favorable conditions, the effect of crossbreeding is on average live weight gain of about 10-15% and feed payment 8- 10%. It is proved that to date there has been no clear pattern of a positive trend of change in the yield of piglets per sow in breeding pigs. Analysis of the procedure for receiving funds under the state program for partial reimbursement of the cost of breeding animals purchased for further reproduction shows that in 2017 compensation for breeding pigs and boars of domestic origin ("elite" class) was 20% of the planned due to lack of state budget funds for appropriate measures . In 2018, this amount of compensation was increased to UAH 5,000 per head. It was found that the unit cost of live weight of a breeding animal in 2017 was 2-2.5 times higher than in commercial pig farms. The high cost of production of breeding animals is due to higher feed costs due to components of the diet of mainly foreign production. It is obvious that the difficult financial situation determines the low investment attractiveness of the breeding pig industry for both domestic and foreign investors. Key words: breeding pig breeding, animal productivity, breeding breeders, market dynamics, import of breeding pigs, production cost, state support programs.

ADAPTIVE TRANSFORMATIONS OF A FOX’S KNEE JOINT LIGAMENTS UNDER CHANGED FUNCTIONAL CONDITIONS

The aim of the research is identification of complex structural changes of the fox’s knee joint ligaments to ensure its high biomechanical potencies. A promising approach for the study of adaptive and compensatory changes of the joint as a multicomponent biomechanical system is to evaluate the structural transformations of its ligaments under conditions of a new functional load. The research was carried out on the basis of the Department of Animal Anato-my and Histology named after Professor A. F. Klimov «Moscow state Academy of Veterinary Medicine and Biotech-nology – MBA named after K. I. Scryabin» and on the basis of JSC «Breeding animal farm «Saltykovsky». The arti-cle presents information about morphological transformations of the lateral and median ligaments of the knee joint that determine its reliable stability. Numerous structural changes of the fox ligaments during cage housing is re-flected, which decrease in the thickness of bundles of collagen fibers and their compositional density compared to the standard structure (in wild individuals). Morphological transformations that can significantly reduce the strength and elasticity resistance of the ligaments and the biomechanical potential of the joint are shown. 23 fox individuals, including 15 individuals of the cage housing and 8 representatives of the natural habitat were studied. The re-search used a comprehensive methodological approach, including anatomical preparation, light microscopy of histological sections, scanning electron microscopy, micromorphometry and statistical analysis of the obtained digi-tal data. According to the results of the study, it was found that fox individuals from natural biocenosis, have colla-gen structures of the lateral ligaments different in waving and packing density from ones of cage housing, which can contribute to ensuring their biomechanical domination.

Breeding techniques to form final parent groups of sows (F1) with a high adaptive ability

Global experience related to increased pork production shows that breeding work has become an integral part of the technology, since it is impossible to further improve breeding and performance traits of animals without it and therefore the greater production of better quality pork. The paper presents the results of the breeding process arrangement for creation of F1 parental pig. Substantiation of breeding techniques when creating the final parental groups of sows (F1) with a high adaptive ability was based on complex methods to evaluate breeding animals, including traditional methods of domestic breeding and breeding indices. Evaluation of combination compatibility of breed-linear hybrids with the use of breeding indices during mating of parent initial forms allows to determine efficiency of using Yorkshire sows with Landrace boars, and Landrace sows with Yorkshire boars (final parental sows (F1)), which had significantly better reproductive indicators in comparison with BLW х BM genotype sows. Particularly efficient compatibility was recorded when selecting Yorkshire sows and Landrace boars. It was determined that in modern conditions an increase in the efficiency of breeding and pedigree work in pig breeding was based on the use of genetic methods, including: individual assessment of pedigree species according to the main breeding traits, evaluation of genome of each breeding animal, and use of breeding indices allowing to identify the true genetic potential of animals and predict performance traits of their offspring. The research relevance lies in using the innovative methodology for comprehensive assessment of combination compatibility of parental pairs, unified for pig husbandries and pig farms, which will make it possible to 10% accelerate the breeding process of forming the final parental forms of sows, ensure obtaining offspring with predictable reproductive, fattening and meat traits, and good adaptive ability.

Efficiency of probiotics use in treatment of calves

The comprehensive approach to solving the issue of the animal raising intensification, the integrated part of which is herd breeding, animal yield preservation and its development provision, conditioned the enhancement of the research works aimed at further investigation of young stock diseases, features of their digestion development, search of efficient preventive and treatment measures, feeds and feed additives for simulating animal growth and development. The main objective of this study was the exploration of comparative therapeutic efficiency of the use of in treatment of calves, in acute digestive disturbances in the complex of conventional treatments. The experiment was carried out in the conditions of Danilovsky Complex of Semenovsky Stud Farm CJSC of Medvedev District in Mari El Republic in calves aged 2-4 days with clinical signs of digestive dysfunction. 3 experimental groups of calves were formed observing the principles of analogues. Calves of the first and the second experimental groups, in addition to the treatment regimen adopted at the farm, were given with milk or milk at the rate of 6 grams and 4 grams, respectively per one calf once a day. and biochemical analysis was carried out in all experimental calves at the beginning of the experiment and 5, 10 and 15 days after the treatment start. The experience demonstrated that has a normalizing effect on protein, carbohydrate, mineral metabolisms, and pH balance. The positive effect of this drug on animals is conditioned by the effect of it selectively stimulates the growth of useful intestinal normal flora by inhibiting pathogenic flora of colon.

Economic activity of the Yakuts in the conditions and climate change and “deficit of cold temperature”: traditional practices and challenges of modernity

This article examines the questions pertaining to economic activity of the Yakuts in the conditions of climate change and “deficit of cold temperatures. Such aspects of agricultural activity as horse breeding, animal husbandry, hunting, and horticulture. An attempt is made to assess the scale of influence of changing climate upon the traditional life and industries of horse breeders, animal farmers, and hunters of the rural areas of Sakha Republic (Yakutia), as well as examine which difficulties they face and how they respond to the challenges of modernity. The acquired field materials are collected through the methods of overt observation, in-depth interview, audio, photo and video records. The attention is focused on the rural population who show concern over the situation of their households under such fast-paced circumstances: they do not know what to expect from nature next year, and many of them are not capable of handling the emerged problems. The main conclusions consist in revelation of certain problems in the life of rural population in the territories of Sakha Republic that emerge due to the climatic changes.

26 Defining a robust sow: How increases in sow productivity impact the biology of reproduction, lactation and body composition

Considerable emphasis has been placed on genetic selection for increased litter size, number of pigs weaned and quality and size of piglets weaned. Concurrently, selection goals have also focused on improved GF and kg of pork produced. Productivity of sows and the total U.S. pork production system has continued to improve annually. Data from USDA-ERS and summarized by the National Pork Board (2018) for the years 2001 to 2017 showed an annual linear increase of 0.12 pigs born (R2 = 0.95), pigs slaughtered per breeding animal increased 0.29 head annually (R2 = 0.92) and kg of pork produced per breeding animal showed an annual linear increase of 37.5 kg (R2 = 0.96). It has also been shown that overtime genetic selection, feeding programs and management have resulted in increased ADG, gain:feed and lean efficiency while changes in ADFI were not reported (Fix et al., 2010). Genetic improvement has increased the metabolic demand on sows requiring an increased focus on the management of sow weight and condition. These competing demands require today’s sow to be more robust, as an individual sow’s increased productivity can negatively influence body weight loss, body composition, follicle and oocyte development, and lactation yield.