An Interview with Mansi Srivastava

Mansi Srivastava is a John L. Loeb Associate Professor of the Natural Sciences at Harvard University. This year, she was awarded the Elizabeth D. Hay New Investigator Award by the Society of Developmental Biology, which recognizes new group leaders who have performed outstanding research in developmental biology during the early stages of their independent career. Mansi's research focusses on investigating wound response and stem cell biology during regeneration in an evolutionary context. We talked to Mansi to discover how she feels about receiving this award, and about her career and her activities outside of the lab.

Lipases and carboxylesterases are involved in interspecific pheromone differences between two moth species

Moth sex pheromones are a classical model for studying sexual selection. Females produce a species-specific pheromone blend that attracts males. Revealing the enzymes involved in the interspecific variation in blend composition is key for understanding the evolution of these sexual communication systems. The nature of the enzymes involved in the variation of acetate esters, which are prominent compounds in moth pheromone blends, remains unclear. We identified enzymes involved in acetate metabolism in two closely related species: Heliothis (Chloridea) subflexa and H. (C.) virescens, which differ in production of acetate esters. Through comparative transcriptomic analyses and CRISPR/Cas9 knockouts, we showed that two lipases and two esterases induce lower levels of acetate esters in female pheromones. To place our findings in an evolutionary context, we explored the molecular evolution of related lipases and esterases in Lepidoptera. Together, our results show that lipases and carboxylesterases are unexpectedly involved in tuning Lepidoptera pheromones composition.

The Remarkable Evolutionary Plasticity of Coronaviruses by Mutation and Recombination: Insights for the COVID-19 Pandemic and the Future Evolutionary Paths of SARS-CoV-2

Coronaviruses (CoVs) constitute a large and diverse subfamily of positive-sense single-stranded RNA viruses. They are found in many mammals and birds and have great importance for the health of humans and farm animals. The current SARS-CoV-2 pandemic, as well as many previous epidemics in humans that were of zoonotic origin, highlights the importance of studying the evolution of the entire CoV subfamily in order to understand how novel strains emerge and which molecular processes affect their adaptation, transmissibility, host/tissue tropism, and patho non-homologous genicity. In this review, we focus on studies over the last two years that reveal the impact of point mutations, insertions/deletions, and intratypic/intertypic homologous and non-homologous recombination events on the evolution of CoVs. We discuss whether the next generations of CoV vaccines should be directed against other CoV proteins in addition to or instead of spike. Based on the observed patterns of molecular evolution for the entire subfamily, we discuss five scenarios for the future evolutionary path of SARS-CoV-2 and the COVID-19 pandemic. Finally, within this evolutionary context, we discuss the recently emerged Omicron (B.1.1.529) VoC.

Pretensive Shared Reality

Imaginative pretend play is often thought of as the domain of young children, yet adults regularly engage in elaborated, fantastical, social-mediated pretend play. We draw on multiple examples, but focus on Table-Top Role Playing games (TTRPG) - and specifically, the most popular and enduring table-top role-playing games, Dungeons & Dragons (D&D) - as a primary example of such play. We describe imaginative play in adults via the term ‘pretensive shared reality'; Shared Pretensive Reality describes the ability of a group of individuals to employ a range of higher-order cognitive functions to explicitly and implicitly share representations of a bounded fictional reality in predictable and coherent ways, such that this constructed reality may be explored and invented/embellished with shared intentionality in an ad hoc manner. Pretensive Shared Reality facilitates multiple individual and social outcomes, including generating personal and group-level enjoyment or mirth, the creation or maintenance of social groups, or the safe exploration of individual self concepts (such as alternative expression of a players sexual or gender identity). Importantly, Pretensive Shared Reality (both within the specific context of table-top role-playing games, and other instances) are primarily co-operative and co-creative. Our conception links the widespread existence and forms of adult imaginative play to childhood pretense, places it within a developmental and evolutionary context, and argues that pretensive shared realities - which underpin many forms of imaginative culture - are an important topic of study unto themselves, and may be utilized to provide methodological insight into a variety of psychological domains.

The grain quality of wheat wild relatives in the evolutionary context

Key message We evaluated the potential of wheat wild relatives for the improvement in grain quality characteristics including micronutrients (Fe, Zn) and gluten and identified diploid wheats and the timopheevii lineage as the most promising resources. Abstract Domestication enabled the advancement of civilization through modification of plants according to human requirements. Continuous selection and cultivation of domesticated plants induced genetic bottlenecks. However, ancient diversity has been conserved in crop wild relatives. Wheat (Triticum aestivum L.; Triticum durum Desf.) is one of the most important staple foods and was among the first domesticated crop species. Its evolutionary diversity includes diploid, tetraploid and hexaploid species from the Triticum and Aegilops taxa and different genomes, generating an AA, BBAA/GGAA and BBAADD/GGAAAmAm genepool, respectively. Breeding and improvement in wheat altered its grain quality. In this review, we identified evolutionary patterns and the potential of wheat wild relatives for quality improvement regarding the micronutrients Iron (Fe) and Zinc (Zn), the gluten storage proteins α-gliadins and high molecular weight glutenin subunits (HMW-GS), and the secondary metabolite phenolics. Generally, the timopheevii lineage has been neglected to date regarding grain quality studies. Thus, the timopheevii lineage should be subject to grain quality research to explore the full diversity of the wheat gene pool.

Structure as an innovation

The purpose of the chapter is to present basic theoretical fundaments in the field of structurally significant formations that companies apply in the process of their business operations, market projections and innovative changes. Organizational forms and structures are presented in the evolutionary context of the time parameter, applying a chronological historical landmark. Knowledge of the variety of possibilities for structural dimensions of the functional manifestations of business activity provides an expanded range of possible solutions. Basic definition formulations are considered. The emphasis is on the organization and organizational structural forms, changes in their practical dimensions in accordance with the changes in purely managerial knowledge and the needs of business practice for innovative changes in various functions in the scope of company’s activity. With the help of various creative and purely managerial approaches, through cognitive brainstorming activities, a set of analytical, inductive-deductive, case and simulation methods, students gain new knowledge, practical skills and visionary views on the presented issues—by combining theoretical statements, empirical factology and analytical assignments based on the principle of learning by doing. The topic draws students’ attention to the study, selection and construction of internal units and structures, including innovative ones, as part of the necessary business projections for the construction of sustainable processes to generate new value and positive impacts for the environment and stakeholders.

STAT proteins: a kaleidoscope of canonical and non-canonical functions in immunity and cancer

STAT proteins represent an important family of evolutionarily conserved transcription factors that play key roles in diverse biological processes, notably including blood and immune cell development and function. Classically, STAT proteins have been viewed as inducible activators of transcription that mediate cellular responses to extracellular signals, particularly cytokines. In this ‘canonical’ paradigm, latent STAT proteins become tyrosine phosphorylated following receptor activation, typically via downstream JAK proteins, facilitating their dimerization and translocation into the nucleus where they bind to specific sequences in the regulatory region of target genes to activate transcription. However, growing evidence has challenged this paradigm and identified alternate ‘non-canonical’ functions, such as transcriptional repression and roles outside the nucleus, with both phosphorylated and unphosphorylated STATs involved. This review provides a revised framework for understanding the diverse kaleidoscope of STAT protein functional modalities. It further discusses the implications of this framework for our understanding of STAT proteins in normal blood and immune cell biology and diseases such as cancer, and also provides an evolutionary context to place the origins of these alternative functional modalities.

Die Stammgruppe der Fächerflügler (Insecta, Strepsiptera)

Die Fächerflügler (Strepsiptera) sind mit nur ca. 600 beschriebenen rezenten Arten eine kleine, parasitische Gruppe der holometabolen Insekten. Fossilfunde sind selten, aber in den letzten Jahren hat sich die Kenntnis der Stammgruppe der Strepsiptera durch die Entdeckung gut erhaltener Arten aus kreidezeitlichem burmesischem Bernstein und eozänem baltischen Bernstein stark vermehrt. Bis auf ganz wenige Ausnahmen, wie eine fossile Primärlarve aus burmesischem Bernstein und ein spätes weibliches Larvenstadium der †Mengeidae aus baltischem Bernstein, sind nur Männchen bekannt. Diese Bernsteinfossilien haben wesentlich zum Verständnis der Evolution der Strepsiptera im späten Mesozoikum und Känozoikum beigetragen. Die Stammgruppenvertreter der Fächerflügler werden vorgestellt und in einen evolutiven Kontext eingeordnet. The stem-group of the twisted-winged parasites (Insecta, Strepsiptera) Abstract: With only about 600 described extant species, the twisted-winged parasites (Strepsiptera) are a small, parasitic group of holometabolous insects. Fossil records of Strepsiptera are rare, but in the last years the knowledge of the stem group has greatly increased with the discovery of well-preserved species from Cretaceous Burmese amber and Eocene Baltic amber. With very few exceptions, such as a fossil primary larva from Burmese amber and a late female larval stage of the †Mengeidae from Baltic amber, only males are known. These amber fossils have greatly contributed to the understanding of the evolution of Strepsiptera in the late Mesozoic and Cenozoic. The stem group representatives of the twisted-winged parasites are described and placed in an evolutionary context.

A Bird's-Eye View: Core Knowledge in Wild North Island Robins of New Zealand

<p>A prominent psychological theory on early cognitive development is Spelke’s Core Knowledge (CK) hypothesis (Spelke, Breinlinger, Macomber, & Jacobson, 1992), which posits that human infants, and possibly other species, are guided by innate understandings of how object movements, classification and quantification are governed by physical laws and, further, how agents are capable of perceptions and purposive action. CK is a set of cognitive building blocks, which serve as the foundation for more complex cognition such as acquisition and use of symbol systems pertaining to language and mathematics (Spelke, 2000). Evidence points to four core systems of knowledge: representation of number, object, space (or geometry) and agency. Investigation of spontaneous CK in nonhuman species in the wild is fundamental to understanding the ecological validity and evolutionary context for a set of systems that is proposed to be universally embedded. The bold, inquisitive manner, naïve fearlessness and unique insect caching behaviour of wild North Island robins (Petroica longipes) presents a unique opportunity to identify and characterise CK in a new model system. Six studies were conducted with the aim of investigating core developmental cognition in robins. The first three studies focused on perception of numerical quantity. Study 1 investigated the ability to discriminate between both large and small quantities, finding that robins successfully discriminate between unusually large quantities independent of ratio. Study 2 explored quantity discrimination in which summation of items is spatially distributed across an array, and found that while robins perform successfully with small numbers, the task presented substantially more cognitive demand. Study 3 measured robins’ reactions to computation by presenting simple addition and subtraction problems in a Violation of Expectancy (VoE) paradigm, finding that robins search longer when presented with a mathematically incongruent scenario. The last three studies focused on perception of agency. Study 4 investigated robins’ response to gaze direction in humans in a competitive paradigm, and found that they were sensitive to human gaze direction in all conditions but one. Study 5 explored perception of physical capability in humans, and results indicated that limb visibility significantly influences pilfering choice. Study 6 examined robins’ perception of animacy in prey, finding that in a VoE paradigm, robins’ expectation of hidden prey continuity varies depending on mobility and animacy. Taken together, the results of these six studies suggest that while supportive of fundamental characteristics defining basic Core Knowledge in many ways, some unique results in the cognitive abilities of this biologically naïve species shed new light on our growing understanding of the shared basis of cognition. A deeper look at avian performance in core developmental tasks, especially in a naïve wild population, can offer new insights into sweeping evolutionary theories that underpin basic cognitive mechanisms.</p>

A Bird's-Eye View: Core Knowledge in Wild North Island Robins of New Zealand

<p>A prominent psychological theory on early cognitive development is Spelke’s Core Knowledge (CK) hypothesis (Spelke, Breinlinger, Macomber, & Jacobson, 1992), which posits that human infants, and possibly other species, are guided by innate understandings of how object movements, classification and quantification are governed by physical laws and, further, how agents are capable of perceptions and purposive action. CK is a set of cognitive building blocks, which serve as the foundation for more complex cognition such as acquisition and use of symbol systems pertaining to language and mathematics (Spelke, 2000). Evidence points to four core systems of knowledge: representation of number, object, space (or geometry) and agency. Investigation of spontaneous CK in nonhuman species in the wild is fundamental to understanding the ecological validity and evolutionary context for a set of systems that is proposed to be universally embedded. The bold, inquisitive manner, naïve fearlessness and unique insect caching behaviour of wild North Island robins (Petroica longipes) presents a unique opportunity to identify and characterise CK in a new model system. Six studies were conducted with the aim of investigating core developmental cognition in robins. The first three studies focused on perception of numerical quantity. Study 1 investigated the ability to discriminate between both large and small quantities, finding that robins successfully discriminate between unusually large quantities independent of ratio. Study 2 explored quantity discrimination in which summation of items is spatially distributed across an array, and found that while robins perform successfully with small numbers, the task presented substantially more cognitive demand. Study 3 measured robins’ reactions to computation by presenting simple addition and subtraction problems in a Violation of Expectancy (VoE) paradigm, finding that robins search longer when presented with a mathematically incongruent scenario. The last three studies focused on perception of agency. Study 4 investigated robins’ response to gaze direction in humans in a competitive paradigm, and found that they were sensitive to human gaze direction in all conditions but one. Study 5 explored perception of physical capability in humans, and results indicated that limb visibility significantly influences pilfering choice. Study 6 examined robins’ perception of animacy in prey, finding that in a VoE paradigm, robins’ expectation of hidden prey continuity varies depending on mobility and animacy. Taken together, the results of these six studies suggest that while supportive of fundamental characteristics defining basic Core Knowledge in many ways, some unique results in the cognitive abilities of this biologically naïve species shed new light on our growing understanding of the shared basis of cognition. A deeper look at avian performance in core developmental tasks, especially in a naïve wild population, can offer new insights into sweeping evolutionary theories that underpin basic cognitive mechanisms.</p>