This study evaluated the microstructure and mechanical properties of friction stir welded lap joints. Inconel 600 and SS 400 as experimental materials were selected, and friction stir welding was carried out at tool rotation speed of 200 rpm and welding speed of 100 mm/min. Applying the friction stir welding was notably effective to reduce the grain size of the stir zone, as a result, the average grain size of Inconel 600 was reduced from 20 μm in the base material to 8.5 μm in the stir zone. Joint interface between Inconel 600 and SS 400 showed a sound weld without voids and cracks. Also, the hook, along the Inconel 600 alloy from SS 400, was formed at advancing side, which directly affected an increase in peel strength. In this study, we systematically discussed the evolution on microstructure and mechanical properties of friction stir lap jointed Inconel 600 and SS 400.