scholarly journals Versatile and High-Throughput Microfluidics Platform for Dorsal Cell Mechanics

2019 ◽  
Vol 116 (3) ◽  
pp. 121a
Author(s):  
Seungman Park ◽  
Yoon Ki Joo ◽  
Yun Chen
Keyword(s):  
High Throughput ◽  
Cell Mechanics ◽  
Microfluidics Platform

scholarly journals Versatile and High-throughput Force Measurement Platform for Dorsal Cell Mechanics

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Seungman Park ◽  
Yoon Ki Joo ◽  
Yun Chen
Keyword(s):  
High Throughput ◽  
Cell Mechanics ◽  
Force Measurement ◽  
Time Lapse ◽  
Dorsal Side ◽  
Single Particle Tracking ◽  
Single Experiment ◽  
Molecular Binding ◽  
Force Magnitude

Abstract We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted on the cell can be modulated ranging from ~14 pN to 2 nN to perturb various force-dependent processees in cells. Time-lapse images were acquired to record events due to such perturbation. The values of various mechanical parameters are subsequently obtained by single particle tracking. Up to 50 events can be measured simultaneously in a single experiment. Integrating the microfluidic techniques with the analytic framework established in computational fluid dynamics, our method is physiologically relevant, reliable, economic and efficient.

High-throughput measurements of cell mechanics using atomic force microscopy with micro-patterned substrates

MHS2013 ◽  
2013 ◽  
Author(s):  
Ryosuke Takahashi ◽  
Kaori Kuribayashi-Shigetomi ◽  
Masahiro Tsuchiya ◽  
Agus Subagyo ◽  
Kazuhisa Sueoka ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Throughput ◽  
Cell Mechanics ◽  
Patterned Substrates ◽  
Force Microscopy ◽  
Atomic Force

Massively parallel microfluidics platform for storage and ultra-high-throughput screening

2002 ◽  
Author(s):  
Colin J. H. Brenan ◽  
Tom Morrison ◽  
Kristine Stone ◽  
Tara Heitner ◽  
Arrin Katz ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Massively Parallel ◽  
Microfluidics Platform

scholarly journals High-throughput ballistic injection nanorheology to measure cell mechanics

2012 ◽  
Vol 7 (1) ◽  
pp. 155-170 ◽  
Author(s):  
Pei-Hsun Wu ◽  
Christopher M Hale ◽  
Wei-Chiang Chen ◽  
Jerry S H Lee ◽  
Yiider Tseng ◽  
...  
Keyword(s):  
High Throughput ◽  
Cell Mechanics

scholarly journals Evaluation of a high-throughput, microfluidics platform for performing TaqMan™ qPCR using formalin-fixed paraffin-embedded tumors

Bioanalysis ◽  
2013 ◽  
Vol 5 (13) ◽  
pp. 1623-1633 ◽  
Author(s):  
Ping Li ◽  
Elena Grigorenko ◽  
Vince Funari ◽  
Edward Enright ◽  
Hao Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Microfluidics Platform

scholarly journals An Automated High-throughput Array Microscope for Cancer Cell Mechanics

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jeremy A. Cribb ◽  
Lukas D. Osborne ◽  
Kellie Beicker ◽  
Matthew Psioda ◽  
Jian Chen ◽  
...  
Keyword(s):  
Cancer Cell ◽  
High Throughput ◽  
Cell Mechanics
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