Inhibitory effects of starvation on prothoracic gland cell DNA synthesis during the last larval instar of the silkworm,Bombyx mori

2008 ◽  
Vol 309A (7) ◽  
pp. 399-406 ◽  
Author(s):  
Chien‐Hung Chen ◽  
Shi‐Hong Gu
Keyword(s):  
Dna Synthesis ◽  
Bombyx Mori ◽  
Gland Cell ◽  
Larval Instar ◽  
Prothoracic Gland ◽  
Inhibitory Effects ◽  
Silkworm Bombyx Mori

In vitro action of prothoracicotropic hormone on RNA-synthesis in the prothoracic gland of the silkworm, Bombyx mori

1986 ◽  
Vol 32 (8) ◽  
pp. 711-717 ◽  
Author(s):  
Yasuo Aizono ◽  
Noboru Matsuo ◽  
Yoshihiro Yoshida ◽  
Gunki Funatsu ◽  
Masaru Funatsu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Rna Synthesis ◽  
Prothoracic Gland ◽  
Prothoracicotropic Hormone ◽  
Silkworm Bombyx Mori

Proteomic identification of the silkworm (Bombyx mori L) prothoracic glands during the fifth instar stage

2009 ◽  
Vol 29 (2) ◽  
pp. 121-129 ◽  
Author(s):  
Xiao-feng Wu ◽  
Xing-hua Li ◽  
Wan-fu Yue ◽  
Bhaskar Roy ◽  
Guang-li Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Cell Function ◽  
Storage Proteins ◽  
Primary Source ◽  
Prothoracic Gland ◽  
Japanese Strain ◽  
Proteomic Approach ◽  
Bombyx Mori L ◽  
The Difference ◽  
Silkworm Bombyx Mori

Although the ecdysteroid of the silkworm had been studied for decades, the proteome of the prothoracic gland, the primary source of ecdysteroid hormones, has not been studied previously. In the present paper, we utilized a proteomic approach to investigate the fifth instar prothoracic gland during the growth and development of the silkworm, Bombyx mori L. The two-dimensional electrophoresis results showed that the majority of proteins were acidic proteins, especially concentrated in the area of 25–65 kDa, with pI values of between 4 and 7, and the difference was not distinct. When compared with Qiufeng (Japanese strain), the interspecific distinction was larger than the intraspecific distinction, and 19 particular spots, excized from the third, fifth and ninth days of p50 (Chinese strain) and Qiufeng were subjected to MALDI-TOF–MS (matrix-assisted laser-desorption ionization–time-of-flight MS) analysis. We sorted them into seven catagories: energetics and/or metabolism, storage proteins, protection, lipid metabolism, signal transduction, cell function and unknown function proteins. Of these proteins, arginine methyltransferase is discussed as playing an important role in regulating the activation of ecdysteroidogenesis via transcription or translation.

scholarly journals STUDIES ON THE POSTERIOR SILK GLAND OF THE SILKWORM, BOMBYX MORI

1968 ◽  
Vol 38 (3) ◽  
pp. 574-588 ◽  
Author(s):  
Yutaka Tashiro ◽  
Takashi Morimoto ◽  
Shiro Matsuura ◽  
Sunao Nagata
Keyword(s):  
Bombyx Mori ◽  
Early Stage ◽  
Larval Instar ◽  
Silk Gland ◽  
Exponential Increase ◽  
Wet Weight ◽  
Rough Er ◽  
Electron Microscopical ◽  
Silkworm Bombyx Mori ◽  
Posterior Silk Gland

Growth of the posterior silk gland and biosynthesis of fibroin during the fifth larval instar of the silkworm, Bombyx mori, have been studied. In accordance with the exponential increase in the wet weight of the gland, the amounts of DNA, RNA, protein, and lipids per animal increased rapidly in the early stage of the fifth instar (0–96 hr). Biosynthesis of fibroin, on the contrary, mainly proceeds in the later stage of the fifth instar (120–192 hr). Electron microscopical observations have shown that, in the very early stage (0–12 hr), a number of free ribosomes exist in the cytoplasm. Rough endoplasmic reticulum (ER) with closely spaced cisternae was also observed. Then rough ER starts to proliferate rapidly, and at the same time lamellar ER is rapidly or gradually transformed into vesicular or tubular forms. In the later stage of the fifth instar (120–192 hr), the cytoplasm is mostly filled with tubular or vesicular ER. Golgi vacuoles, free vacuoles (fibroin globules), and mitochondria are also observed. It is concluded that in the early stage of the fifth instar the cellular structures necessary for the biosynthesis of fibroin are rapidly formed, while in the later stage the biosynthesis of fibroin proceeds at a maximum rate and utilizes these structures.

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