Studies on rumen metabolism. I.—effect of lipids on the concentration of ammonia, total and individual volatile fatty acids in the rumen

1964 ◽  
Vol 15 (5) ◽  
pp. 274-282 ◽  
Author(s):  
J. A. Robertson ◽  
J. C. Hawke
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Metabolism

RUMINAL DIGESTION OF ALFALFA STRAINS SELECTED FOR SLOW AND FAST INITIAL RATES OF DIGESTION

1985 ◽  
Vol 65 (1) ◽  
pp. 157-161 ◽  
Author(s):  
H. KUDO ◽  
K.-J. CHENG ◽  
M. R. HANNA ◽  
R. E. HOWARTH ◽  
B. P. GOPLEN ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Soluble Protein ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Soluble Carbohydrates ◽  
Hydrogen Ions ◽  
Feeding Trial ◽  
Rumen Metabolism ◽  
Medicago Sativa L ◽  
Ruminal Digestion

A feeding trial, using six fistulated sheep, was conducted to evaluate two strains of alfalfa that were selected for slow and fast initial rates of digestion (IRD). To determine how these strains affect rumen metabolism, samples of rumen fluid were collected before feeding, as well as 2 h and 4 h after feeding. Concentrations of soluble protein, soluble carbohydrates, chlorophyll, volatile fatty acids and hydrogen ions were significantly lower in rumen fluid from sheep fed slow-IRD alfalfa compared to sheep fed fast-IRD alfalfa. These results show that the bloat-causing potential of alfalfa might be reduced through further breeding for slow-IRD strains. Key words: Bloat, legumes, Medicago sativa L., nylon bag, chlorophyll, pH

Possible contribution of protozoa to differences in rumen metabolism between cattle breeds

1992 ◽  
Vol 43 (8) ◽  
pp. 1795 ◽  
Author(s):  
JC O'Kelly ◽  
WG Spiers
Keyword(s):  
Fatty Acids ◽  
Population Density ◽  
Volatile Fatty Acids ◽  
Essential Fatty Acids ◽  
Plasma Concentrations ◽  
Metabolizable Energy ◽  
Rumen Temperature ◽  
Once Daily ◽  
Rumen Metabolism ◽  
Body Tissues

Brahman (B) and Hereford (H) cattle were used to study protozoal population density of ruminal fluid (RF) in relation to some aspects of rumen and body metabolism. Steers were fed restricted intakes of lucerne hay (HQ) or low-quality hay (LQ) in each of eight experiments. In the 8 h period following the once-daily feeding of HQ the number of entodiniomorphs declined by 50%, while the number of holotrichs increased by 60% before reducing to pre-feeding levels. There were no significant rises and falls in the numbers of protozoa of steers fed LQ once daily nor in those fed either HQ or LQ at hourly intervals. The numbers of entodiniomorphs, dasytrichs, total protozoa, pH and bacterial content of RF were higher and rumen temperature lower in B than in H. Urinary allantoin excretion used as an index of the total microbial population in the rumen distinguished between diets but not between breeds. The concentrations of propionic, butyric, isobutyric and isovaleric acids in RF and of glucose in plasma were higher in B than in H. The concentrations of all classes of long-chain fatty acids contained in the protozoa and bacteria of RF and of cholesterol and essential fatty acids in plasma were higher in B than in H. The amount of dry matter disappearing from nylon bags in the rumen of steers fed HQ did not differ between breeds, but was higher in B than in H fed LQ. Elevation of pH to peak values in RF with an accompanying disappearance of protozoa and volatile fatty acids during fasting from either HQ or LQ occurred more rapidly in B than in H. The plasma concentrations of non-esterified fatty acids were higher in B than in H during fasting. It is proposed that at the same level of intake of either HQ or LQ: (1) the higher protozoal population density in RF of B contributes to quantitative breed differences in the end products of digestion, (2) that B achieve and maintain a higher body weight because more metabolizable energy and essential nutrients are supplied from the rumen to their body tissues, and (3) after the same period of feed deprivation B have fasted for a longer period of time because the residual feed in their rumens is fermented at a more rapid rate.

Some effects of chloral hydrate on rumen fermentation and digestion in sheep

1982 ◽  
Vol 99 (1) ◽  
pp. 215-224 ◽  
Author(s):  
J. C. Mathers ◽  
E. L. Miller
Keyword(s):  
Fatty Acids ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Chloral Hydrate ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Long Chain Fatty Acids ◽  
Ether Extract ◽  
Rumen Metabolism ◽  
Long Chain

SUMMARYTwo experiments were carried out with adult sheep to investigate the effects of chloral hydrate (CH) on rumen metabolism and on the sites and extent of digestion.Experiment 1 confirmed that CH is a very potent methane inhibitor; doses of 1 or 4 g/day reduced methane production to 4% of that observed in the absence of the drug. In both experiments, CH administration altered the proportions of volatile fatty acids in rumen fluid. The main changes were an increase in propionic acid and a decrease in acetic acid with smaller increases in the proportion of isovaleric, valeric and caproic acids. Stoichiometric calculations indicated that only 21% of the hydrogen saved by prevention of methanogenesis was diverted to the synthesis of additional propionic and butyric acids and the fate of the remaining hydrogen was not determined unequivocally.CH had little effect on the extent of biohydrogenation in the rumen of the long-chain fatty acids in dried grass.The apparent digestibility of ether extract was reduced (P< 0·05) by CH but those of other proximate constituents and energy were little affected. There were no s gnificant effects of CH on the extent of digestion of dry matter, organic matter, non-ammonia nitrogen or ash between the mouth and abomasum, abomasum and terminal ileum or ileum and anus.

Net mesenteric and portal absorption and rumen metabolism of volatile fatty acids in forage-fed steers receiving intraruminal propionic acid

1993 ◽  
Vol 1993 ◽  
pp. 54-54 ◽  
Author(s):  
C.J. Seal ◽  
D.S. Parker
Keyword(s):  
Fatty Acids ◽  
Oxygen Consumption ◽  
Energy Consumption ◽  
Gastrointestinal Tract ◽  
Propionic Acid ◽  
Oxidative Metabolism ◽  
Volatile Fatty Acids ◽  
Rumen Metabolism ◽  
Total Body ◽  
Total Body Oxygen

The ability of the gut tissues to absorb nutrients and the extent to which these are then metabolised within the gastrointestinal tract plays a central role in regulating the supply of metabolites to the liver and consequently influences the availability of nutrients for hepatic and peripheral tissue use. Energy consumption by the portal drained viscera is extensive and may account for 25% of total body oxygen consumption (Huntington and Reynolds, 1987). Substrates available for oxidative metabolism include volatile fatty acids (VFA) produced in the rumen and large intestine as addition to arterial supply of these nutrients. The aim of this experiment was to investigate the influence of increased propionate availability on metabolism of VFA in the rumen and the gastrointestinal tract.

A note on the effect of polyethylene particles on rumen metabolism of strees

1974 ◽  
Vol 18 (1) ◽  
pp. 105-108 ◽  
Author(s):  
J. Fordyce ◽  
M. Kay
Keyword(s):  
Fatty Acids ◽  
Feed Intake ◽  
Volatile Fatty Acids ◽  
Rumen Metabolism ◽  
Polyethylene Particles ◽  
Ad Libitum

SUMMARYExperiments were carried out to determine the effects of giving 1·36 kg of ‘RuffTabs’, polythene particles of dimension 1·27 × 0·63 × 0·95 cm3 (RT) to castrated male cattle (steers) given an all-concentrate diet, on the outflow of fluid from the rumen, on rumination and on the molar proportions of volatile fatty acids produced in the rumen.RT reduced the flow of fluid from the rumen of steers, given a restricted amount of an all-concentrate diet, from 4·65 to 2·75% rumen volume/hr. RT had no effect on the total time spent daily in ruminating and eating. The molar proportions of volatile fatty acids were unaffected by RT in steers which were given the diet ad libitum but when RT was given to steers offered a restricted allowance of feed there was a highly significant (P < 0·001) reduction in acetic and an increase in butyric, valeric and caproic acids.The feed intake of the steers was unaffected by RT.

scholarly journals Efisiensi Metabolisme Rumen Pakan Berbasis Jerami Padi Amoniasi dan Konsentrat yang Disuplementasi Ekstrak Daun Waru (Hibiscus tiliaceus) (In-Vitro)

2021 ◽  
Vol 21 (2) ◽  
pp. 113-121
Author(s):  
Muhamad Bata ◽  
Sri Rahayu ◽  
Mela Oktora
Keyword(s):  
Fatty Acids ◽  
Rice Straw ◽  
Volatile Fatty Acids ◽  
Block Design ◽  
Ethanol Extract ◽  
Fermentation Products ◽  
Methane Gas ◽  
Rumen Metabolism ◽  
Ethanolic Extracts

ABSTRACT. Penelitian bertujuan mengkaji pengaruh interaksi taraf suplementasi ekstrak etanol daun waru (Hibiscus tiliaceus) dan rasio jerami padi amoniasi dan konsentrat terhadap efisiensi metabolisme rumen dan total protozoa rumen secara in vitro. Penelitian eksperimental menggunakan Rancangan Acak Kelompok pola faktorial 3 x 3. Faktor pertama adalah imbangan bahan kering (BK), jerami padi amoniasi (JPA) dan konsentrat yaitu 45:55 (R1), 55:45 (R2), dan 65:35 (R3). Faktor kedua adalah taraf suplementasi ekstrak etanol daun waru yaitu 0 ppm (E0), 200 ppm (E1), dan 400 ppm (E2). Variabel yang diamati adalah total protozoa dan produk fermentasi rumen yaitu sintesis protein mikroba (SPM), volatile fatty acids (VFA), N-NH3, gas metan, rasio Asetat/Propionat (A/P), dan efisiensi konversi glukosa menjadi VFA (EVFA). Hasil penelitian menunjukkan bahwa tidak terdapat interaksi (P0,05) antara rasio JAP: konsentrat dan taraf ekstrak etanol daun waru terhadap N-NH3, total VFA, asam asetat, asam propionat, asam butirat, rasio A/P, dan (EVFA). Rasio JAP: konsentrat R2 menghasilkan EVFA dan SPM lebih tinggi (P0,05) jika dibandingkan dengan R1 dan R3, akan tetapi metan dan protozoa lebih rendah (P0,05). Suplementasi ekstrak etanol daun waru sampai 400 ppm meningkatkan secara liner (P0,05) EVFA, SPM, dan propionate, akan tetapi menurunkan rasio A/P, gas metan, total protozoa, dan asetat. Suplementasi ekstrak etanol daun waru pada taraf 400 ppm dan rasio JAP: konsentrat 55:45% BK dapat meningkatkan efisiensi metabolisme rumen. (Rumen metabolism efficiency of rice straw ammoniation and concentrate based diet supplemented with Hibiscus tiliaceus leaf extract in vitro) ABSTRAK. The aim of this study was to examine the effect of the interaction between the level of supplementation of ethanolic extracts of waru leaves (Hibiscus tiliaceus) and the ratio of ammoniated rice straw and concentrate on rumen metabolism efficiency and total rumen protozoa in vitro. The research was carried out experimentally designed using a 3 x 3 factorial randomized block design. The first factor was the dry matter (DM) ratio of ammoniated rice straw (ARS) and the concentrates were 45:55 (R1), 55:45 (R2), and 65:35 (R3). The second factor was the supplementation level of ethanolic extracts of waru leaves of 0, 200, and 400 ppm for E0, E1, and E2, respectively. The variables measured were total protozoa and rumen fermentation products, namely microbial protein synthesis (MSP), volatile fatty acids (VFA), N-NH3, methane gas, acetate/propionate (A/P) ratio, and efficiency of glucose conversion to VFA (EVFA). The results showed that there was no interaction (P 0.05) between the ratio of ARS:concentrate and the ethanol extract level of waru leaves on N-NH3, total VFA, acetic acid, propionic acid, butyric acid, A/P ratio, and (EVFA). The ratio of ARS: concentrate on R2 resulted in higher EVFA and SPM (P0.05) compared to R1 and R3, but lower methane and protozoa (P0.05). Supplementation of waru leaf ethanol extract up to 400 ppm increased linearly (P0.05) EVFA, SPM, and propionate, but decreased A/P ratio, methane gas, total protozoa, and acetate. Supplementation of the ethanolic extracts of waru leaves at the level of 400 ppm and the ratio of ARS:concentrate is 55: 45% increase the efficiency of rumen metabolism.

scholarly journals Rate of Rumen Metabolism of C14-Labeled Alfalfa Hay as Determined by Specific Activity of Rumen Volatile Fatty Acids, Blood, Urine, and Feces

1964 ◽  
Vol 47 (12) ◽  
pp. 1346-1351 ◽  
Author(s):  
I.S. Yadava ◽  
E.E. Bartley ◽  
L.R. Fina ◽  
C.L. Alexander ◽  
R.M. Meyer ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Specific Activity ◽  
Rumen Metabolism ◽  
Alfalfa Hay

Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Microbial Consortia ◽  
Chain Elongation ◽  
Desulfovibrio Vulgaris ◽  
Rrna Gene ◽  
Selective Enrichment ◽  
Microbial Network ◽  
Cost Efficient ◽  
Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

МЕТАБОЛИТЫ ПРОБИОТИЧЕСКИХ БАКТЕРИЙ ОТВЕЧАЮТ ЗА ЭФФЕКТИВНОСТЬ ДЕЙСТВИЯ ПРОБИОТИКА "ПРОФОРТ®"

2019 ◽  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Broiler Chicks ◽  
Bacillus Sp

МЕЛИКИДИ В.Х., ТЮРИНА Д.Г., СЕЛИВАНОВ Д.Г., НОВИКОВА Н.И. ООО «БИОТРОФ», Санкт-Петербург Аннотация: Приведены данные исследования методом газожидкостной хроматомасс-спектрометрии метаболитов, синтезируемых пробиотическими бактериями, входящими в состав кормовой добавки «Профорт®». Проведен опыт в условиях интенсивного промышленного птицеводства по применению кормовой добавки «Профорт®» (50 тыс. голов бройлеров в группе). Среди метаболитов пробиотических штаммов Enterococcus sp. и Bacillus sp. обнаружены такие полезные вещества, как молочная кислота, уксусная, пропионовая и другие короткоцепочечные (летучие) жирные кислоты, активные пептиды. Результаты зоотехнического опыта показали, что при скармливании бройлерам пробиотика «Профорт®» (500 г/т) живая масса при убое в 40 дней была выше контроля на 6,9%, конверсия корма улучшилась на 3,0%, а европейский индекс продуктивности бройлеров - на 5,69%. Ключевые слова: ПРОБИОТИКИ, МЕТАБОЛИТЫ, ЛЕТУЧИЕ ЖИРНЫЕ КИСЛОТЫ, ЦЫПЛЯТА-БРОЙЛЕРЫ,ПРОДУКТИВНОСТЬ, PROBIOTICS, METABOLITES, VOLATILE FATTY ACIDS, BROILER CHICKS,PRODUCTIVITY

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