High Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model

2021 ◽  
Author(s):  
Olga Gordeeva
Keyword(s):  
Image Analysis ◽  
Embryoid Body ◽  
Proliferation And Differentiation ◽  
Embryoid Body Differentiation ◽  
Differentiation Model

scholarly journals A single cell transcriptional portrait of embryoid body differentiation and comparison to progenitors of the developing embryo

2018 ◽  
Vol 31 ◽  
pp. 201-215 ◽  
Author(s):  
Abby Spangler ◽  
Emily Y. Su ◽  
April M. Craft ◽  
Patrick Cahan
Keyword(s):  
Single Cell ◽  
Embryoid Body ◽  
Embryoid Body Differentiation

scholarly journals GCN5 Regulates FGF Signaling and Activates Selective MYC Target Genes during Early Embryoid Body Differentiation

2018 ◽  
Vol 10 (1) ◽  
pp. 287-299 ◽  
Author(s):  
Li Wang ◽  
Evangelia Koutelou ◽  
Calley Hirsch ◽  
Ryan McCarthy ◽  
Andria Schibler ◽  
...  
Keyword(s):  
Target Genes ◽  
Embryoid Body ◽  
Fgf Signaling ◽  
Embryoid Body Differentiation

scholarly journals Receptor Protein Tyrosine Phosphatase Gamma, Ptpγ, Regulates Hematopoietic Differentiation

Blood ◽  
1997 ◽  
Vol 90 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Claudio Sorio ◽  
Paola Melotti ◽  
Daniela D'Arcangelo ◽  
Jeannine Mendrola ◽  
Bruno Calabretta ◽  
...  
Keyword(s):  
Embryoid Body ◽  
Es Cells ◽  
Tyrosine Phosphatase ◽  
Embryonic Stem ◽  
Surface Protein ◽  
Embryoid Bodies ◽  
Receptor Protein ◽  
Hematopoietic Differentiation ◽  
Normal Number ◽  
Embryoid Body Differentiation

Murine embryonic stem (ES) cells have been a useful model system for the study of various aspects of hematopoietic differentiation. Because we had observed a sharp peak of expression of the receptor tyrosine phosphatase gamma (Ptpγ) gene between 14 and 18 days of ES-derived embryoid body differentiation, we investigated the effect of perturbation of expression of the Ptpγ gene on ES cell differentiation, first by analyzing the effect of Ptpγ overexpression. The murine full-length Ptpγ cDNA in an expression vector was transfected into ES-D3 cells and stably transfected clones were isolated. Ptpγ was expressed as an approximately 230-kD cell surface protein, and differentiating ES clones that overexpressed Ptpγ gave rise to a normal number of hematopoietic colonies, approximately 1 CFU per 100 cells. There was, however, a significant increase of expression of early hematopoietic markers in colonies from Ptpγ overexpressing ES cells. To confirm that the pertubation of hematopoietic differentiation was a result of Ptpγ overexpression, we isolated ES stem cell clones expressing Ptpγ antisense constructs and assayed embryoid bodies for the presence of hematopoietic precursors. We observed a complete absence of methylcellulose colonies, indicating absence of hematopoietic lineages. Results of these experiments point to an essential role for Ptpγ in hematopoietic differentiation.

A Modified SMART-Seq Method for Single-Cell Transcriptomic Analysis of Embryoid Body Differentiation

2021 ◽  
Author(s):  
Jianqun Zheng ◽  
Ying Ye ◽  
Qiushi Xu ◽  
Wei Xu ◽  
Wensheng Zhang ◽  
...  
Keyword(s):  
Single Cell ◽  
Embryoid Body ◽  
Transcriptomic Analysis ◽  
Embryoid Body Differentiation

Rotary Suspension Culture Enhances the Efficiency, Yield, and Homogeneity of Embryoid Body Differentiation

Stem Cells ◽  
2007 ◽  
Vol 25 (9) ◽  
pp. 2224-2234 ◽  
Author(s):  
Richard L. Carpenedo ◽  
Carolyn Y. Sargent ◽  
Todd C. McDevitt
Keyword(s):  
Suspension Culture ◽  
Embryoid Body ◽  
Embryoid Body Differentiation

Gradient release of cardiac morphogens by photo-responsive polymer micelles for gradient-mediated variation of embryoid body differentiation

2017 ◽  
Vol 5 (26) ◽  
pp. 5206-5217 ◽  
Author(s):  
Mukesh K. Gupta ◽  
Daniel A. Balikov ◽  
Yunki Lee ◽  
Edward Ko ◽  
Christopher Yu ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Embryoid Body ◽  
Polymer Micelles ◽  
Responsive Polymer ◽  
Embryoid Body Differentiation

Illustration of photo-gradient mediated release of retinoic acid from polymer micelles upon photo-irradiation (365 nm).

scholarly journals miR-208b Regulates Rabbit Preadipocyte Proliferation and Differentiation

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 890
Author(s):  
Jiahao Shao ◽  
Ting Pan ◽  
Jie Wang ◽  
Tao Tang ◽  
Yanhong Li ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Target Gene ◽  
Cell Counting ◽  
Signal Pathways ◽  
Preadipocyte Differentiation ◽  
Proliferation And Differentiation ◽  
Oil Red O Staining ◽  
Oil Red O ◽  
Differentiation Model ◽  
Gene Mrna

microRNAs (miRNAs) play an important role in gene regulation in animals by pairing with target gene mRNA. Many miRNAs are differentially expressed in the adipose tissue, often with conserved expression. In our study, we found that miR-208b expression was observed differently in the preadipocyte differentiation model. When miR-208b was overexpressed in the preadipocyte differentiation model, the overexpressed group displayed higher expression of PPARγ and FABP4—the markers of preadipocyte differentiation. Oil Red O staining revealed that the count of lipid droplets was increased in the overexpressed group. When the expression of miR-208b was inhibited, the above indicators showed an opposite trend. Moreover, results from both 5-ethynyl-2’-deoxyuridine (EDU) and cell counting kit (CCK) analysis showed that miR-208b promoted the proliferation of preadipocyte. Expression of gene CSNK2A2, a direct miR-208b target, was downregulated in the overexpressed group, providing a possible link to multiple signal pathways. Overall, our data indicate that miR-208b play a positive regulatory effect on the proliferation and differentiation of rabbit preadipocyte.

Application of digital image analysis on histological images of a murine embryoid body model for monitoring endothelial differentiation

2020 ◽  
Vol 216 (11) ◽  
pp. 153225
Author(s):  
Nabi Mousavi ◽  
Marie Birkvig Raft ◽  
Sarah Line Bring Truelsen ◽  
Vera Timmermans ◽  
Jacob Thastrup ◽  
...  
Keyword(s):  
Image Analysis ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Embryoid Body ◽  
Endothelial Differentiation ◽  
Body Model ◽  
Histological Images
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