Microscopical Detection of Cell Death Processes During Scots Pine Zygotic Embryogenesis

2020 ◽  
pp. 223-237
Author(s):  
Jaana Vuosku ◽  
Suvi Sutela
Keyword(s):  
Cell Death ◽  
Scots Pine ◽  
Zygotic Embryogenesis

scholarly journals Expression of catalase and retinoblastoma-related protein genes associates with cell death processes in Scots pine zygotic embryogenesis

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Jaana Vuosku ◽  
Suvi Sutela ◽  
Johanna Kestilä ◽  
Anne Jokela ◽  
Tytti Sarjala ◽  
...  
Keyword(s):  
Cell Death ◽  
Scots Pine ◽  
Zygotic Embryogenesis ◽  
Related Protein

Patterning during somatic embryogenesis in Scots pine in relation to polar auxin transport and programmed cell death

2011 ◽  
Vol 109 (3) ◽  
pp. 391-400 ◽  
Author(s):  
Malin Abrahamsson ◽  
Silvia Valladares ◽  
Emma Larsson ◽  
David Clapham ◽  
Sara von Arnold
Keyword(s):  
Cell Death ◽  
Somatic Embryogenesis ◽  
Programmed Cell Death ◽  
Scots Pine ◽  
Auxin Transport ◽  
Polar Auxin Transport

scholarly journals Consistency of Polyamine Profiles and Expression of Arginine Decarboxylase in Mitosis during Zygotic Embryogenesis of Scots Pine

2006 ◽  
Vol 142 (3) ◽  
pp. 1027-1038 ◽  
Author(s):  
Jaana Vuosku ◽  
Anne Jokela ◽  
Esa Läärä ◽  
Mira Sääskilahti ◽  
Riina Muilu ◽  
...  
Keyword(s):  
Scots Pine ◽  
Arginine Decarboxylase ◽  
Zygotic Embryogenesis

scholarly journals Thermospermine Synthase (ACL5) and Diamine Oxidase (DAO) Expression Is Needed for Zygotic Embryogenesis and Vascular Development in Scots Pine

2019 ◽  
Vol 10 ◽  
Author(s):  
Jaana Vuosku ◽  
Riina Muilu-Mäkelä ◽  
Komlan Avia ◽  
Marko Suokas ◽  
Johanna Kestilä ◽  
...  
Keyword(s):  
Scots Pine ◽  
Diamine Oxidase ◽  
Vascular Development ◽  
Zygotic Embryogenesis

DNA nick end labeling: a reliable marker for apoptosis?

1995 ◽  
Vol 53 ◽  
pp. 962-963
Author(s):  
Anne F. Bushnell ◽  
Sarah Webster ◽  
Lynn S. Perlmutter
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Apoptotic Cell ◽  
Morphological Characteristics ◽  
Detection Methods ◽  
Tissue Preparation ◽  
Preparation Methods ◽  
Dna Laddering ◽  
Disease States ◽  
Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

Paracrystalline Aggregates of Psoriatic Keratin and Their Relation to Normal Keratin Structure

1985 ◽  
Vol 43 ◽  
pp. 680-681
Author(s):  
S. Trachtenberg ◽  
P.M. Steinert ◽  
B.L. Trus ◽  
A.C. Steven
Keyword(s):  
Cell Death ◽  
Stratum Corneum ◽  
Intermediate Filament ◽  
Skin Disease ◽  
Amorphous Matrix ◽  
Terminal Differentiation ◽  
Proteolytic Degradation ◽  
Horny Layer ◽  
Chronic Skin ◽  
Chronic Skin Disease

During terminal differentiation of vertebrate epidermis, certain specific keratin intermediate filament (KIF) proteins are produced. Keratinization of the epidermis involves cell death and disruption of the cytoplasm, leaving a network of KIF embedded in an amorphous matrix which forms the outer horny layer known as the stratum corneum. Eventually these cells are shed (desquamation). Normally, the processes of differentiation, keratinization, and desquamation are regulated in an orderly manner. In psoriasis, a chronic skin disease, a hyperkeratotic stratum corneum is produced, resulting in abnormal desquamation of unusually large scales. In this disease, the normal KIF proteins are diminished in amount or absent, and other proteins more typical of proliferative epidermal cells are present. There is also evidence of proteolytic degradation of the KIF.

The early development of antenna and antennal sensilla in the noctuid moth, Agrotis segetum (Insecta: Lepidoptera)

1990 ◽  
Vol 48 (3) ◽  
pp. 502-503
Author(s):  
Eric Hallberg ◽  
Lina Hansén
Keyword(s):  
Cell Death ◽  
Stem Cell ◽  
Early Development ◽  
Larval Stage ◽  
Pupal Stage ◽  
Agrotis Segetum ◽  
Antennal Sensilla ◽  
Noctuid Moth ◽  
Standard Procedures

The antennal rudiments in lepidopterous insects are present as disks during the larval stage. The tubular double-walled antennal disk is present beneath the larval antenna, and its inner layer gives rise to the adult antenna during the pupal stage. The sensilla develop from a cluster of cells that are derived from one stem cell, which gives rise to both sensory and enveloping cells. During the morphogenesis of the sensillum these cells undergo major transformations, including cell death. In the moth Agrotis segetum the pupal stage lasts about 14 days (temperature, 25°C). The antennae, clearly seen from the exterior, were dissected and fixed according to standard procedures (3 % glutaraldehyde in 0.15 M cacaodylate buffer, followed by 1 % osmiumtetroxide in the same buffer). Pupae from day 1 to day 8, of both sexes were studied.

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