Effect of Arabinogalactan-Proteins and Chitinases on Somatic Embryogenesis

2000 ◽  
pp. 109-119 ◽  
Author(s):  
Marc Kreuger ◽  
Arjon van Hengel ◽  
Sacco de Vries
Keyword(s):  
Somatic Embryogenesis ◽  
Arabinogalactan Proteins

scholarly journals Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 70
Author(s):  
Ana D. Simonović ◽  
Milana M. Trifunović-Momčilov ◽  
Biljana K. Filipović ◽  
Marija P. Marković ◽  
Milica D. Bogdanović ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Developmental Plasticity ◽  
Leaf Explants ◽  
Arabinogalactan Proteins ◽  
Culture Conditions ◽  
Current Status ◽  
Special Focus ◽  
Root Explants ◽  
Centaurium Erythraea

Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum of secondary metabolites with confirmed healing properties. Centaury is an emerging model in plant developmental biology due to its vigorous regenerative potential and great developmental plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic embryos. When SE is initiated from centaury root explants, the process occurs spontaneously (on hormone-free medium), directly (without the callusing phase), and the somatic embryos are of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is indirect (preceded by callusing). Histological observations and culture conditions are compared in these two systems. The changes in antioxidative enzymes were followed during SE from the leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were analyzed using a variety of approaches. The newest and preliminary results, including centaury transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed as perspectives of centaury research.

scholarly journals Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of a Daucus carota (L.) Hypocotyl in Which Somatic Embryogenesis Was Induced

2020 ◽  
Vol 21 (21) ◽  
pp. 8126
Author(s):  
Michał Kuczak ◽  
Ewa Kurczyńska
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Fate ◽  
Cell Walls ◽  
Arabinogalactan Proteins ◽  
Cell Wall Composition ◽  
Cell Reprogramming ◽  
Daucus Carota L ◽  
Negative Marker ◽  
Spatio Temporal ◽  
Wall Components

Changes in the composition of the cell walls are postulated to accompany changes in the cell’s fate. We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate. Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis. Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming.

scholarly journals N-Acetylglucosamine and Glucosamine-Containing Arabinogalactan Proteins Control Somatic Embryogenesis

2001 ◽  
Vol 125 (4) ◽  
pp. 1880-1890 ◽  
Author(s):  
Arjon J. van Hengel ◽  
Zewdie Tadesse ◽  
Peter Immerzeel ◽  
Henk Schols ◽  
Ab van Kammen ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Arabinogalactan Proteins

Arabinogalactan-proteins stimulate somatic embryogenesis and plant propagation of Pelargonium sidoides

2016 ◽  
Vol 152 ◽  
pp. 149-155 ◽  
Author(s):  
Stefanie Duchow ◽  
Renate I. Dahlke ◽  
Thomas Geske ◽  
Wolfgang Blaschek ◽  
Birgit Classen
Keyword(s):  
Somatic Embryogenesis ◽  
Arabinogalactan Proteins ◽  
Plant Propagation ◽  
Pelargonium Sidoides

scholarly journals Use of Arabinogalactan Proteins in Coconut (Cocos Nucifera L.) Tissue Culture: An Alternative Approach for Improved Tissue Response

CORD ◽  
2011 ◽  
Vol 27 (2) ◽  
pp. 8
Author(s):  
S C Fernando
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Cocos Nucifera ◽  
Arabinogalactan Proteins ◽  
Tissue Response ◽  
Arabic Gum ◽  
Positive Effects ◽  
Larch Wood ◽  
Coconut Kernel

Arabinogalactan proteins (AGPs) are extra-cellular proteins involved in plant growth and development. The effects of these proteins on in vitro response of different species are well documented. This study assessed for the first time the role of AGPs on tissue culture of coconut, a highly recalcitrant species in vitro. Coconut (Sri Lanka Tall) plumules were cultured in medium containing 2, 4-dichlorophenoxyaceticacid (2,4-D) for callogenesis. Calli were multiplied by subculturing onto freshly prepared callusing medium. Somatic embryos were induced by transferring of calli to medium with 2/3 concentration of initial 2,4-D and matured by subsequent transferring to medium without any hormones. Embryos were converted in the presence of gibberelic acid. Arabic gum (1-50 mgL-1) was used as the main source of AGP. Alternatively, different AGP sources like Larch wood gum, carrot seeds and defatted coconut kernel were also assessed. Arabic gum in callusing medium resulted in early callogenesis from 20-40% plumules compared to 10% in the control. However, it did not have a significant effect on final callusing efficiency. Its presence in somatic embryogenesis medium showed a significantly higher mass of embryogenic structures per an embryogenic clump (33.3-50.5mg) when compared to the control (26.9mg).The best result was obtained at 10mgL-1 Arabic gum. All AGP sources except Larch wood gum had positive effects on somatic embryogenesis. Among them, defatted coconut kernel (25-50mgL-1) showed the best results giving 70% cultures with embryogenic structures compared to 37% in control. However, so far, the positive effect of AGP did not reflect in plant regeneration efficiency.

Promotive and inhibitory effects of diverse arabinogalactan proteins on Daucus carota L. somatic embryogenesis

Planta ◽  
1997 ◽  
Vol 203 (2) ◽  
pp. 188-195 ◽  
Author(s):  
Marcel A. J. Toonen ◽  
Ed D. L. Schmidt ◽  
Ab van Kammen ◽  
Sacco C. de Vries
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Arabinogalactan Proteins ◽  
Inhibitory Effects ◽  
Daucus Carota L

Arabinogalactan proteins are essential in somatic embryogenesis of Daucus carota L.

Planta ◽  
1993 ◽  
Vol 189 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Marc Kreuger ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Arabinogalactan Proteins ◽  
Daucus Carota L
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