Comparative Study of the Antioxidant Protection Level in the Duroc Boar Blood Based on the Measurements of Active Products of the Thiobarbituric Acid

2021 ◽  
pp. 500-506
Author(s):  
Sergei Zaitsev ◽  
Alexey Mishurov ◽  
Nadezhda Bogolyubova
Keyword(s):  
Comparative Study ◽  
Thiobarbituric Acid ◽  
Antioxidant Protection ◽  
Protection Level

scholarly journals Genderspecific features of antioxidant protection and efficacy of atorvastatin in patients with stable forms of coronary heart disease

2017 ◽  
Vol 95 (5) ◽  
pp. 398-403
Author(s):  
K. Yu. Bondar ◽  
O. L. Belaya ◽  
O. M. Lazutina ◽  
I. S. Mikhailova ◽  
T. V. Smorkalova ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Heart Disease ◽  
Heart Disease ◽  
Plasma Lipids ◽  
Thiobarbituric Acid ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Paramagnetic Resonance ◽  
Stable Forms ◽  
Gender Specific

The gender-specific features of cardiovascular diseases (CVD) are actively being discussed which puts on the agenda the development of a differentiated approach to the treatment of cardiac diseases in men and women. Аim. To assess the gender-specific features of the antioxidant status and antioxidative activity of atorvastatin in patients with stable forms of coronary heart disease (CHD) and dyslipidemia (DLP). Material and methods. The study included 102 patients with stable forms of CHD divided into 2 groups according to sex and 40 healthy individuals. The generally accepted methods were used to determine plasma lipids, products of their peroxidation (diene conjugates, DC) and the products reacting with 2-thiobarbituric acid (TBA-RP), the antioxidant enzymes glutathione peroxidase (GP) and superoxide dismutase (SOD) in erythrocytes; the activity of the ceruloplasmin/transferrin antioxidant system (AOA AOS CP/TF) in blood plasma was measured by electron paramagnetic resonance and the final metabolites of nitric oxide with the use of the Griss reagent. The endothelial function was studied by ultrasound with the assessment of endothelium-dependent vasodilatation as described by D. Celermajer et al. (1992). All parameters were evaluated before and 6 months after the onset of the treatment. Results. The men with CHD, unlike women, underwent a reduction of AOA AOS CP/TF by 10%, SOD by 12% and GP by 19% (p<0.05) as well as excessive accumulation of DC up to 9% and TBA-RP to 11% (p<0.05). The homocysteine level in men, was 1.5 times higher (p=0.01), and that of final nitric oxide metabolites 12% lower (p=0.03) than in women. Atorvastatin administered for 6 months as a component of combined cardiac therapy more significantly increased the activity of AOS CP/TF and endothelium-dependent vasodilatation in men than in women. Conclusion. CHD is associated with significant sex differences in processes of lipid peroxidation - antioxidant protection, metabolism of nitric oxide, found, dilatation and anti-oxidation effect of atorvastatin.

scholarly journals Correction of oxidative stress in rat blood with magnesium citrate upon experimental diabetes

2016 ◽  
Vol 71 (1) ◽  
pp. 81-84
Author(s):  
O. Shatynska ◽  
R. Iskra
Keyword(s):  
Oxidative Stress ◽  
Experimental Diabetes ◽  
Thiobarbituric Acid ◽  
Antioxidant Systems ◽  
Lipid Hydroperoxides ◽  
Antioxidant Protection ◽  
Magnesium Citrate ◽  
Antioxidant Parameters ◽  
Peroxidation Processes ◽  
Alloxan Monohydrate

The investigation of the influence of different concentrations of magnesium citrate (100, 250 and 500 mg/kg body weight) on the lipid peroxidation processes and the activity of the antioxidant system in red blood cell of rats with experimental diabetes was conducted. Diabetes was caused by the single introduction of a 5% solution of alloxan monohydrate. The activity of the enzymes of antioxidant protection, catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase and the content of reduced glutathione were studied in red blood cells. The increasing levels of lipid hydroperoxides and thiobarbituric acid reactive substances against the backdrop of decreased activity of enzymes of antioxidant protection was observed in rats with experimental diabetes. Magnesium citrate, which for four weeks, together with drinking water was added to the diet of the animals, showed a positive normalizing effect. Particularly, the contents of LPO and TBARS declined, and activity of antioxidant systems of protection increased significantly. Our data shows that the magnesium citrate supplements can partly restore the antioxidant parameters and reduce oxidative stress in rats with alloxan-induced diabetes.

scholarly journals CHANGES OF PARAMETRES OF FREE-RADICAL STATUS, OF ANTIOXIDANT PROTECTION AND MORPHOLOGIC CHANGES OF ERYTHROCYTES OF PERIPHERAL BLOOD OF PREGNANT WOMEN OF THE FIRST TRIMESTER WITH CYTOMEGALOVIRUS INFECTION

2018 ◽  
Vol 1 (68) ◽  
pp. 57-62 ◽  
Author(s):  
Наталия Ишутина ◽  
Nataliya Ishutina ◽  
Ирина Андриевская ◽  
Irina Andrievskaya
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Pregnant Women ◽  
Cytomegalovirus Infection ◽  
Thiobarbituric Acid ◽  
First Trimester ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
First Trimester Of Pregnancy ◽  
Morphologic Changes

The aim is to study the features of changes of free-radical status, antioxidant protection and morphologic changes of erythrocytes of the peripheral blood of pregnant women of the first trimester with exacerbation of cytomegalovirus infection (CMVI). 35 pregnant women with exacerbation of CMVI in the first trimester of gestation (the main group) and 35 pregnant women without this pathology of the same age and term as the patients of the main group were examined. Erythrocytes of the venous blood stabilized by heparine as well as blood plasma became the material of the study. There has been studied the condition of lipid peroxidation (LP) by the contents of diene conjugates and active products reacting with thiobarbituric acid; the state of nonenzymatic antioxidant protection by the contents of α-tocopherol as well as of the enzyme of erythrocytes (superoxide dismutase); morphologic changes of erythrocytes. It was found out that the exacerbation of CMVI in the first trimester of pregnancy is associated with activation of LP processes which include modification of not only primary, reverse stages of this process – the increase of the concentration of diene conjugates (p<0.001), but they lead to an increased formation of secondary active products reacting with thiobarbituric acid (p<0.001), intensifying the destructive action on the membrane of erythrocytes. Primary and secondary products of LP against the decrease of concentration of erythrocyte superoxide dismutase (p<0.001) and α-tocopherol (p<0.001) are accumulated in increasing concentrations and become potential factors of erythrocytes damage causing their functional and metabolic inferiority. Metabolic disturbances and damages of cellular membranes lead to the increase of population of echinocytes, target-like and degenerative forms of erythrocytes, which can be accompanied by a high probability of intravascular hemolysis and contribute to the development of hemic hypoxia complicating the infectious process and the course of pregnancy. Within the present hyperoxidation and suppression of antioxidant protection that lead to the worsening of the rheology of blood, women with exacerbation of CMVI in the first trimester of pregnancy should be prescribed the medicine stabilizing the lipid exchange.

Plasma lipoprotein peroxidation potential: a test to evaluate individual susceptibility to peroxidation

1991 ◽  
Vol 37 (10) ◽  
pp. 1756-1758 ◽  
Author(s):  
M A Arshad ◽  
S Bhadra ◽  
R M Cohen ◽  
M T Subbiah
Keyword(s):  
Clinical Status ◽  
Lipid Peroxide ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Peroxidation Of Lipids ◽  
Gas Liquid Chromatography ◽  
Antioxidant Protection ◽  
Degenerative Diseases ◽  
Individual Susceptibility ◽  
Cupric Acetate

Abstract Peroxidation of lipids is believed to play a key role in various degenerative diseases. However, few simple tests are able to detect individual susceptibility or resistance to peroxidation. Measurement of the basal concentrations of lipid peroxides in plasma is not satisfactory because they are so low. Therefore, we developed a test to determine susceptibility of whole plasma to metal/H2O2-catalyzed peroxidation. Incubation of 300-500 microL of plasma with H2O2/cupric acetate resulted in the formation of products from fatty acids (malonaldehyde, measured by thiobarbituric acid assay) and cholesterol (predominantly cholest-3,5-dien-7-one, measured by gas-liquid chromatography). In the presence of Cu2+, formation of malonaldehyde and cholest-3,5-dien-7-one increased at least 10-fold over basal values. Lipid peroxide (malonaldehyde) and cholesterol oxide concentrations after peroxidation were significantly higher (P less than 0.01) in diabetic plasma than in normal plasma. Because susceptibility to plasma peroxidation represents a balance between pro-oxidant factors and antioxidant protection, this test may be useful in determining individual susceptibility to peroxidation as influenced by nutritional and clinical status.

scholarly journals Oxidative stress in HIV positive children

2017 ◽  
Vol 5 (4) ◽  
pp. 1578
Author(s):  
Bina F. Dias ◽  
Aruna Srinivas
Keyword(s):  
Oxidative Stress ◽  
Comparative Study ◽  
Thiobarbituric Acid ◽  
Hiv Positive ◽  
Age Group ◽  
Normal Children ◽  
Prospective Comparative Study

Background: The objective is to assess oxidative stress by measuring the concentration of malondialdehyde in HIV positive children and compare it with normal children (not suffering from any disease) of the same age group.Methods: In this prospective comparative study, we analysed malondialdehyde in 80 HIV positive children in the age group of 6-12 yrs from lower socio-economic strata and compared the values with 85 normal children not infected by any disease, of the same age group and similar socio-economic strata at L.T.M.M. College. Estimation of Malondialdehyde was carried out by using the method of SADASIVUDU by thiobarbituric acid reaction.Results: The level of Malondialdehyde was significantly higher in HIV positive children than in controls.Conclusions: The increased levels of Malondialdehyde confirm the involvement of oxidative stress in the pathophysiology of this infection in children

scholarly journals BIOCHEMICAL AND MORPHOLOGICAL MARKERS OF EXPERIMENTAL SCOPOLAMINE-INDUCED NEURODEGENERATION AND THE EFFECT OF ENALAPRIL ON THEM

2020 ◽  
Vol 73 (10) ◽  
pp. 2114-2119
Author(s):  
Оlga G. Kmet ◽  
Nаtaliia D. Filipets ◽  
Taras I. Kmet ◽  
Yurii M. Vepriuk ◽  
Kateryna V. Vlasova
Keyword(s):  
Cerebral Cortex ◽  
Thiobarbituric Acid ◽  
The Body ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Morphological Markers ◽  
Peroxide Oxidation ◽  
The Brain ◽  
Reduced Intensity ◽  
Number Of Cells

The aim: Was to study enalapril effect on protein peroxide oxidation and lipids of the cerebral cortex and hippocampus of rats with neurodegeneration pattern. Materials and methods: The experiments were conducted on nonlinear albino male rats 0,18-0,20 kg of the body weight. Modeled neurodegeneration was simulated by intraperitoneal introduction of scopolamine hydrochloride during 27 days in the dose of 1 mg/kg. Enalapril was introduced intraperitoneal in the dose of 1 mg/kg. Results: In rats with modeled scopolamine-induced neurodegeneration in the cerebral cortex and hippocampus under enalapril effect, the content of products, reacting with 2-thiobarbituric acid and proteins of a neutral and major character, decreases, which is indicative of a reduced intensity of lipid and protein peroxide oxidation. Morphologic the number of cells with karyopyknosis signs decreases and a relative staining density of the neuron tigroid substance increases, which is indicative of inhibition of the progress of the cerebral neurodestructive processes under conditions of scopolamine-induced damage. Conclusions: The obtained results are indicative of enalapril ability to improve the examined indices, which is the evidence of increased processes of antioxidant protection and possibility to inhibit neurodegeneration development in case of scopolamine induced lesion of the brain.

scholarly journals Antioxidant protection by haemopexin of haem-stimulated lipid peroxidation

1988 ◽  
Vol 256 (3) ◽  
pp. 861-865 ◽  
Author(s):  
J M Gutteridge ◽  
A Smith
Keyword(s):  
Lipid Peroxidation ◽  
Protoporphyrin Ix ◽  
Thiobarbituric Acid ◽  
Iron Chelator ◽  
Oxygen Radical ◽  
Butylated Hydroxytoluene ◽  
Radical Reactions ◽  
Antioxidant Protection ◽  
Reactive Material ◽  
Chain Breaking

Haem (ferrous protoporphyrin IX) is a reactive low-molecular-mass form of iron able to participate in oxygen-radical reactions that can lead to the degradation of proteins, lipids, carbohydrates and DNA. Oxygen-radical reactions are likely to occur upon tissue damage. Extracellular fluids rely on antioxidant mechanisms different from those found inside the cell, and circulating proteins limit radical reactions by converting pro-oxidant forms of iron into less-reactive forms. Of the compounds tested, only apohaemopexin and the chain-breaking antioxidant butylated hydroxytoluene inhibited (by more than 90%) haemin-stimulated peroxidation as measured by formation of conjugated dienes, thiobarbituric acid-reactive material from linolenic acid or peroxidation-induced phospholipid fluorescence. Haptoglobin, the haemoglobin-binding serum protein, was ineffective. Conversely, only haptoglobin significantly inhibited haemoglobin-stimulated lipid peroxidation. Iron-salt-induced lipid peroxidation was inhibited only by apotransferrin and the iron-chelator desferrioxamine. All lipid peroxidations were inhibited by the radical scavengers butylated hydroxytoluene and propyl gallate. These findings support the concept that transport and conservation of body iron stores are closely linked to antioxidant protection.

Author response for "Laminar distribution of cortical projection neurons to the pulvinar: A comparative study in cats and mice"

2020 ◽  
Author(s):  
Bruno Oliveira Ferreira de Souza ◽  
Éve‐Marie Frigon ◽  
Robert Tremblay‐Laliberté ◽  
Christian Casanova ◽  
Denis Boire
Keyword(s):  
Comparative Study ◽  
Author Response ◽  
Projection Neurons ◽  
Cortical Projection ◽  
Laminar Distribution
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