Bioremediation of Heavy Metals Using the Symbiosis Between Leguminous Plants and Genetically Engineered Rhizobia

2021 ◽  
pp. 303-322
Author(s):  
Yasser A. El-Tahlawy ◽  
Osama A. M. Ali
Keyword(s):  
Heavy Metals ◽  
Genetically Engineered ◽  
Leguminous Plants

scholarly journals Scenario of heavy metal contamination in agricultural soil and its management

2009 ◽  
Vol 1 (1) ◽  
pp. 99-108 ◽  
Author(s):  
A. K. Chopra ◽  
Chakresh Pathak ◽  
G. Prasad
Keyword(s):  
Heavy Metals ◽  
Heavy Metal Contamination ◽  
Complex Structure ◽  
Specific Area ◽  
Genetically Engineered ◽  
Natural Phenomenon ◽  
Mineral Matter ◽  
Rural And Urban ◽  
Precise Knowledge ◽  
Living Organisms

Soil is a complex structure and contains mainly five major components i.e. mineral matter, water, air, organic matter and living organisms. The quantity of these components in the soil does not remain the same but varies with the locality. Soil possesses not only a nucleus position for existence of living being but also ensures their future existence. Therefore, it is essential to make an adequate land management to maintain the quality of soil in both rural and urban soil. The presence of different kinds of heavy metals such as Cd, Cu, Mn, Bi and Zn etc. in trace or in minimum level is a natural phenomenon but their enhanced level is an indicator of the degree of pollution load in that specific area. The precise knowledge of these kinds of heavy metals, their forms and their dependence on soil provides a genuine base for soil management. The heavy metals have potent cumulative properties and toxicity due to which they have a potential hazardous effect not only on crop plants but also on human health. The metal contaminants can be reduced by immobilization of contaminants using macrophytes and also by using genetically engineered microorganisms.

A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia

2002 ◽  
Vol 99 (3) ◽  
pp. 279-293 ◽  
Author(s):  
Rutchadaporn Sriprang ◽  
Makoto Hayashi ◽  
Mitsuo Yamashita ◽  
Hisayo Ono ◽  
Kazuhiko Saeki ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Genetically Engineered ◽  
Leguminous Plant

Cadmium(II) and Lead(II) removal by Chlorella sp. Immobilized and E. coli genetically engineered with mice Metallothionein I

2010 ◽  
Vol 1278 ◽  
Author(s):  
V. Almaguer-Cantú ◽  
L. Morales-Ramos ◽  
K. Arevalo-Niño ◽  
M.T. Garza-González ◽  
I. Balderas-Rentería
Keyword(s):  
Heavy Metals ◽  
Aqueous Solutions ◽  
Metal Concentration ◽  
New Technologies ◽  
Genetically Engineered ◽  
Suitable Material ◽  
E Coli ◽  
Chlorella Sp ◽  
Heavy Metals Ions ◽  
Langmuir Constants

AbstractThe pollution caused by heavy metals is one of the major environmental problems that is imperative to be solved. New technologies, easy to implement and to adapt to any system, deserve special attention and are a focus of this work the ability of Chlorella sp. and E. coli genetically engineered with mice metallothionein I, both immobilized in alginate of calcium to remove Cd(II) and Pb(II) from aqueous solutions was investigated in batch assays for the treatment of diluted aqueous solutions. The kinetics, sorption capacities and sorption percentage were determined. The influence of metal concentration in solution is discussed in the terms of Langmuir isotherm and constants. Sorption capacities increased with increasing metal concentration in solution. For solution containing 300 mg/L of metal, the observed uptake capacities were 94.941±1.094 mgCd/gChlorella., 24.076±2.292 mgCd/gE.coli and 239.17±2.478 mgPb/gChlorella, 37.952±4.245 mgPb/gE.coli. The Langmuir constants to Chlorella sp. were qmax=285.72(mgPb/g), b=0.0276(l/mgPb), qmax=103.65(mgCd/g) and b=0.0005(l/mgCd) while to E. coli were qmax=28.141(mgPb/g), b=0.113(l/mgPb), qmax=24.272(mgCd/g) and b =0.019(1/mgCd). The biomass of the algae showed to have better capacity of metallic sorption that the biomass of the bacteria genetically engineering. The study proved that microorganisms biomass is a suitable material for the removal of the studied heavy metals ions from aqueous solutions, achieving removal efficiencies higher than 90%, and could be considered as a potential material for treating effluent polluted with Cd(II) and Pb(II) ions.

scholarly journals Genetic Engineering of Escherichia coli for Enhanced Uptake and Bioaccumulation of Mercury

2001 ◽  
Vol 67 (11) ◽  
pp. 5335-5338 ◽  
Author(s):  
Weon Bae ◽  
Rajesh K. Mehra ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Heavy Metals ◽  
Metal Binding ◽  
Heavy Metal Contamination ◽  
Genetically Engineered ◽  
Transport Systems ◽  
Limiting Factor ◽  
Binding Motif ◽  
Metal Binding Peptides ◽  
Binding Peptides ◽  
Genetically Engineered Organisms

ABSTRACT Synthetic phytochelatins (ECs) are a new class of metal-binding peptides with a repetitive metal-binding motif, (Glu-Cys) n Gly, which were shown to bind heavy metals more effectively than metallothioneins. However, the limited uptake across the cell membrane is often the rate-limiting factor for the intracellular bioaccumulation of heavy metals by genetically engineered organisms expressing these metal-binding peptides. In this paper, two potential solutions were investigated to overcome this uptake limitation either by coexpressing an Hg2+ transport system with (Glu-Cys)20Gly (EC20) or by directly expressing EC20 on the cell surface. Both approaches were equally effective in increasing the bioaccumulation of Hg2+. Since the available transport systems are presently limited to only a few heavy metals, our results suggest that bioaccumulation by bacterial sorbents with surface-expressed metal-binding peptides may be useful as a universal strategy for the cleanup of heavy metal contamination.

scholarly journals Enhanced Mercury Biosorption by Bacterial Cells with Surface-Displayed MerR

2003 ◽  
Vol 69 (6) ◽  
pp. 3176-3180 ◽  
Author(s):  
Weon Bae ◽  
Cindy H. Wu ◽  
Jan Kostal ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Heavy Metals ◽  
Ice Nucleation ◽  
Genetically Engineered ◽  
Binding Property ◽  
Bacterial Cells ◽  
Toxic Heavy Metals ◽  
Ice Nucleation Protein ◽  
Whole Cell ◽  
In The Wild ◽  
Metalloregulatory Proteins

ABSTRACT The metalloregulatory protein MerR, which exhibits high affinity and selectivity toward mercury, was exploited for the construction of microbial biosorbents specific for mercury removal. Whole-cell sorbents were constructed with MerR genetically engineered onto the surface of Escherichia coli cells by using an ice nucleation protein anchor. The presence of surface-exposed MerR on the engineered strains enabled sixfold-higher Hg2+ biosorption than that found in the wild-type JM109 cells. Hg2+ binding via MerR was very specific, with no observable decline even in the presence of 100-fold excess Cd2+ and Zn2+. The Hg2+ binding property of the whole-cell sorbents was also insensitive to different ionic strengths, pHs, and the presence of metal chelators. Since metalloregulatory proteins are currently available for a wide variety of toxic heavy metals, our results suggest that microbial biosorbents overexpressing metalloregulatory proteins may be used similarly for the cleanup of other important heavy metals.

scholarly journals Toxicity and Bioremediation of Heavy Metals Contaminated Ecosystem from Tannery Wastewater: A Review

2018 ◽  
Vol 2018 ◽  
pp. 1-16 ◽  
Author(s):  
Bernard E. Igiri ◽  
Stanley I. R. Okoduwa ◽  
Grace O. Idoko ◽  
Ebere P. Akabuogu ◽  
Abraham O. Adeyi ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Critical Evaluation ◽  
Cost Effective ◽  
Fold Increase ◽  
Genetically Engineered ◽  
Tannery Wastewater ◽  
Environmental Technology ◽  
Cost Effective Approach ◽  
Removal Of Heavy Metals ◽  
Near Future

The discharge of untreated tannery wastewater containing biotoxic substances of heavy metals in the ecosystem is one of the most important environmental and health challenges in our society. Hence, there is a growing need for the development of novel, efficient, eco-friendly, and cost-effective approach for the remediation of inorganic metals (Cr, Hg, Cd, and Pb) released into the environment and to safeguard the ecosystem. In this regard, recent advances in microbes-base heavy metal have propelled bioremediation as a prospective alternative to conventional techniques. Heavy metals are nonbiodegradable and could be toxic to microbes. Several microorganisms have evolved to develop detoxification mechanisms to counter the toxic effects of these inorganic metals. This present review offers a critical evaluation of bioremediation capacity of microorganisms, especially in the context of environmental protection. Furthermore, this article discussed the biosorption capacity with respect to the use of bacteria, fungi, biofilm, algae, genetically engineered microbes, and immobilized microbial cell for the removal of heavy metals. The use of biofilm has showed synergetic effects with many fold increase in the removal of heavy metals as sustainable environmental technology in the near future.

Cadmium(II) and Lead(II) removal by Chlorella sp. Immobilized and E. coli genetically engineered with mice Metallothionein I

2010 ◽  
Vol 1277 ◽  
Author(s):  
V. Almaguer-Cantú ◽  
L. Morales-Ramos ◽  
K. Arevalo-Niño ◽  
M.T. Garza-González ◽  
I. Balderas-Rentería
Keyword(s):  
Heavy Metals ◽  
Aqueous Solutions ◽  
Metal Concentration ◽  
New Technologies ◽  
Genetically Engineered ◽  
Suitable Material ◽  
E Coli ◽  
Chlorella Sp ◽  
Heavy Metals Ions ◽  
Langmuir Constants

The pollution caused by heavy metals is one of the major environmental problems that is imperative to be solved. New technologies, easy to implement and to adapt to any system, deserve special attention and are a focus of this work the ability of Chlorella sp. and E. coli genetically engineered with mice metallothionein I, both immobilized in alginate of calcium to remove Cd(II) and Pb(II) from aqueous solutions was investigated in batch assays for the treatment of diluted aqueous solutions. The kinetics, sorption capacities and sorption percentage were determined. The influence of metal concentration in solution is discussed in the terms of Langmuir isotherm and constants. Sorption capacities increased with increasing metal concentration in solution. For solution containing 300 mg/L of metal, the observed uptake capacities were 94.941±1.094 mgCd/gChlorella., 24.076±2.292 mgCd/gE.coli and 239.17±2.478 mgPb/gChlorella, 37.952±4.245 mgPb/gE.coli. The Langmuir constants to Chlorella sp. were qmax=285.72(mgPb/g), b=0.0276(l/mgPb), qmax=103.65(mgCd/g) and b=0.0005(l/mgCd) while to E. coli were qmax=28.141(mgPb/g), b=0.113(l/mgPb), qmax=24.272(mgCd/g) and b =0.019(l/mgCd). The biomass of the algae showed to have better capacity of metallic sorption that the biomass of the bacteria genetically engineering. The study proved that microorganisms biomass is a suitable material for the removal of the studied heavy metals ions from aqueous solutions, achieving removal efficiencies higher than 90%, and could be considered as a potential material for treating effluent polluted with Cd(II) and Pb(II) ions.

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