Differential in vitro effects of IL-4, IL-10, and IL-13 on proinflammatory cytokine production and fibroblast proliferation in rheumatoid synovium

2001 ◽  
Vol 20 (2) ◽  
pp. 49-54 ◽  
Author(s):  
Y. Morita ◽  
M. Yamamura ◽  
M. Kawashima ◽  
T. Aita ◽  
S. Harada ◽  
...  
Keyword(s):  
Proinflammatory Cytokine ◽  
Cytokine Production ◽  
Fibroblast Proliferation ◽  
Proinflammatory Cytokine Production ◽  
Rheumatoid Synovium ◽  
In Vitro Effects

Release of (13)-beta-D-Glucan from Depth-type Membrane Filters and Their In Vitro Effects on Proinflammatory Cytokine Production

2003 ◽  
Vol 27 (8) ◽  
pp. 728-735 ◽  
Author(s):  
Atsushi Ohata ◽  
Makoto Usami ◽  
Takashi Horiuchi ◽  
Koichi Nagasawa ◽  
Keiko Kinoshita
Keyword(s):  
Proinflammatory Cytokine ◽  
Cytokine Production ◽  
Proinflammatory Cytokine Production ◽  
Membrane Filters ◽  
In Vitro Effects

scholarly journals Enhanced Proinflammatory Cytokine Production and Immunometabolic Impairment of NK Cells Exposed to Mycobacterium tuberculosis and Cigarette Smoke

2022 ◽  
Vol 11 ◽  
Author(s):  
Yafei Rao ◽  
Xiaoyan Gai ◽  
Yanqing Le ◽  
Jing Xiong ◽  
Yujia Liu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Oxidative Phosphorylation ◽  
Nk Cells ◽  
Cigarette Smoke ◽  
Proinflammatory Cytokine ◽  
Cytokine Production ◽  
Nk Cell ◽  
Proinflammatory Cytokine Production ◽  
Cell Subpopulations

AimSmoker COPD patients with chest radiological signs of prior tuberculosis (TB) showed more severe lung damage, but the mechanisms remain unclear. Emerging evidence has implicated NK cells in the pathogenesis of both COPD and TB. The purpose of this study was to delineate the profile and cytokine production of NK-cell subpopulations and their immunometabolic changes after exposure to both cigarette smoke (CS) and Mycobacterium tuberculosis(MTB).MethodsWe profiled NK-cell subpopulations in terms of percentage and cytokine production by flow cytometry in smoker patients with pulmonary TB (PTB). In an in vitro coexposure model, we investigated proinflammatory cytokine production, glycolytic influx, and oxidative phosphorylation of NK cells under CS extract (CSE) and PPD costimulation.ResultsPeripheral blood NK cells in smoker patients with active PTB (CS+PTB group) showed altered proportion of subpopulations and excessive proinflammatory cytokine expressions. In vitro, CSE- and PPD-coexposed NK-92 cells displayed enhanced proinflammatory cytokine production, concurrent with decreased glycolytic influx and oxidative phosphorylation.ConclusionSmoker patients with active PTB showed enhanced proinflammatory cytokine expression within altered NK cell subpopulations. CSE and PPD coexposure induced heightened cytokine production concurrent with impaired cell metabolism in NK cells. These novel data suggest a potential role of NK cells in the pathogenesis of lung injury in subjects with coexposure to CS and TB.

Ketamine Suppresses Proinflammatory Cytokine Production in Human Whole Blood In Vitro

1999 ◽  
Vol 89 (3) ◽  
pp. 665 ◽  
Author(s):  
Takashi Kawasaki ◽  
Masanori Ogata ◽  
Chika Kawasaki ◽  
Jun-ichi Ogata ◽  
Yoshitaka Inoue ◽  
...  
Keyword(s):  
Whole Blood ◽  
Proinflammatory Cytokine ◽  
Cytokine Production ◽  
Proinflammatory Cytokine Production ◽  
Human Whole Blood

Consistency matters: Consistency in the timing and quality of daily interactions between parents and adolescents predicts production of proinflammatory cytokines in youths

2017 ◽  
Vol 30 (2) ◽  
pp. 373-382 ◽  
Author(s):  
Erika M. Manczak ◽  
Adam K. K. Leigh ◽  
Chia-Ping Chin ◽  
Edith Chen
Keyword(s):  
Relationship Quality ◽  
Proinflammatory Cytokines ◽  
Proinflammatory Cytokine ◽  
Cytokine Production ◽  
Leisure Activities ◽  
Proinflammatory Cytokine Production ◽  
Parent Child Relationship ◽  
Parent Child

AbstractThe current study examined whether consistency in day-to-day interactions between children and parents related to inflammatory cytokine production in youths. One hundred twenty-three parents recorded the daily quality of interactions and timing of leisure activities with their adolescent children for 2 weeks, and the degree of variability in those ratings was calculated. One year later, the production of proinflammatory cytokines in youths’ blood was measured in response to in vitro exposure to lipopolysaccharide (a bacterial product). The results indicate that greater variability in parent–child relationship quality related to greater stimulated proinflammatory cytokine production in youths, above and beyond overall relationship quality. Greater variability in the timing of parent–child leisure activities also predicted greater stimulated proinflammatory cytokine production in youths, regardless of the frequency of interactions. In sum, consistency in both the affective and temporal aspects of parent–child relationships may contribute to inflammatory processes in youth.

Ketamine Suppresses Proinflammatory Cytokine Production in Human Whole Blood In Vitro

1999 ◽  
Vol 89 (3) ◽  
pp. 665 ◽  
Author(s):  
Takashi Kawasaki ◽  
Masanori Ogata ◽  
Chika Kawasaki ◽  
Jun-ichi Ogata ◽  
Yoshitaka Inoue ◽  
...  
Keyword(s):  
Whole Blood ◽  
Proinflammatory Cytokine ◽  
Cytokine Production ◽  
Proinflammatory Cytokine Production ◽  
Human Whole Blood

scholarly journals 862 Dectin-2, a novel target for tumor macrophage reprogramming in cancer immunotherapy

2021 ◽  
Vol 9 (Suppl 3) ◽  
pp. A903-A903
Author(s):  
Justin Kenkel ◽  
Po Ho ◽  
Sameera Kongara ◽  
Karla Henning ◽  
Cindy Kreder ◽  
...  
Keyword(s):  
Proinflammatory Cytokine ◽  
Antigen Processing ◽  
Cytokine Production ◽  
Immune Cell ◽  
Therapeutic Potential ◽  
Costimulatory Molecule ◽  
Mouse Tumor ◽  
Dependent Manner ◽  
Proinflammatory Cytokine Production

BackgroundTumor-associated macrophages (TAMs) are an abundant immune cell population in most cancers that support tumor progression through their immunosuppressive effects. We discovered that TAMs express the pattern recognition receptor Dectin-2 (Clec4n/CLEC6A), an activating C-type lectin receptor (CLR) that binds to high-mannose glycans on fungi and other microbes and induces protective immune responses against infectious disease. Dectin-2 is selectively expressed by myeloid cells, and upon ligation mediates enhanced phagocytosis, antigen processing and presentation, and proinflammatory cytokine production. Given these properties, we evaluated the therapeutic potential of targeting Dectin-2 using naturally derived ligands. We also generated human Dectin-2-targeted agonistic antibodies capable of robustly activating immunosuppressive ”M2” or TAM-like macrophages.MethodsDectin-2 expression was assessed by flow cytometry, immunohistochemistry, and using public databases. Mouse and human monocytes were differentiated into macrophages using recombinant cytokines or tumor-conditioned media, and stimulation was measured following overnight incubation with Dectin-2 ligands or antibodies. Mouse tumor cell lines were implanted into syngeneic hosts and mice were treated with mannan derived from S. cerevisiae via IT or IV administration.ResultsDectin-2 gene expression is minimal in normal human tissues but elevated across many tumor types, including breast, colon, lung, and kidney cancers. Dectin-2 is strongly expressed by macrophages differentiated in vitro and on primary TAMs. The fungal Dectin-2 ligand mannan stimulated proinflammatory cytokine production (e.g. TNFalpha) and costimulatory molecule expression (e.g. CD86) by macrophages in a Dectin-2-dependent manner. Treatment of tumor-bearing mice with mannan mediated tumor regression in multiple syngeneic tumor models, with high rates of tumor clearance in the MB49 bladder cancer model. These effects were Dectin-2 dependent, as efficacy was not observed when a Dectin-2-blocking antibody was co-administered or in knockout mice lacking Dectin-2 signaling components. Furthermore, depletion of either macrophages or T cells impaired efficacy, suggesting that Dectin-2-stimulated TAMs augment anti-tumor T cell responses. Based on these data, we developed novel Dectin-2 targeted agonist antibodies capable of activating human ”M2” or TAM-like macrophages in vitro to produce an array of proinflammatory cytokines and chemokines akin to tumor-destructive ”M1” macrophages.ConclusionsThe data presented demonstrate the therapeutic potential of targeting Dectin-2 using natural ligands or agonistic antibodies as a novel pan-cancer approach for myeloid cell-directed tumor immunotherapy.Ethics ApprovalAll animal studies were performed in accordance with Institutional Animal Care and Use Committee (IACUC)-approved protocols.

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