PKC-dependent IL-6 production and inhibition of IL-8 production by PKC activation in normal human skin fibroblasts under extremely high hydrostatic pressure

Extremophiles ◽  
2002 ◽  
Vol 6 (5) ◽  
pp. 413-418 ◽  
Author(s):  
Sumihiro Koyama ◽  
Masuo Aizawa
Keyword(s):  
Hydrostatic Pressure ◽  
Human Skin ◽  
High Hydrostatic Pressure ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Normal Human Skin ◽  
Normal Human ◽  
Pkc Activation

The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

1984 ◽  
Vol 12 (2) ◽  
pp. 89-97
Author(s):  
Graham R. Elliott ◽  
H.E. Amos ◽  
James W. Bridges
Keyword(s):  
Human Skin ◽  
Psoriasis Patient ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Cell Type ◽  
Normal Human Skin ◽  
Rate Of Growth ◽  
Structural Analogues ◽  
Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

scholarly journals Effect of Pentacyclic Triterpenoids-Rich Callus Extract of Chaenomeles japonica (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 3009 ◽  
Author(s):  
Małgorzata Kikowska ◽  
Małgorzata Chmielewska ◽  
Agata Włodarczyk ◽  
Elżbieta Studzińska-Sroka ◽  
Jerzy Żuchowski ◽  
...  
Keyword(s):  
Human Skin ◽  
Proliferation Rate ◽  
Skin Fibroblasts ◽  
Total Phenolic ◽  
Control Group ◽  
Human Skin Fibroblasts ◽  
Normal Human Skin ◽  
Pentacyclic Triterpenoids ◽  
Antioxidant Power ◽  
Normal Human

The effect of the well-characterized callus extract of Chaenomeles japonica on viability, morphology, and proliferation of normal human skin fibroblasts was investigated. The phytochemical analysis was performed using the ultra-high performance liquid chromatography method. The total phenolic, phenolic acid, and flavonoid contents were determined spectrophotometrically. The antioxidant activity was investigated using the DPPH (1,1-Diphenyl-1-picrylhydrazyl Radical Scavenging), FRAP (Ferric Reducing Antioxidant Power), and CUPRAC (CUPric Reducing Antioxidant Capacity) assays. The callus growth index during passages was high as well as the content of pentacyclic triterpenoids. The microscopic observations of the fibroblast viability, morphology and the evaluation of the proliferation ratio (xCELLigence system) proved that the influence of callus extract on the fibroblasts was dose-dependent. The evaluated level of fibroblasts proliferation rate after 72 h of incubation with callus extract at concentration 12.5 µg L−1 was the highest compared to all the analyzed ligands. Moreover, callus extract administrated for 72 h caused a significant increase in the proliferation rate in comparison with the control group (5.7 ± 0.1 vs. 4.4 ± 0.9; p < 0.01). The preliminary studies carried out may suggest that the callus extract rich in triterpenoids may be a potential source of cosmetic ingredients with a beneficial effect on human skin.

scholarly journals SELECTIVE ANTICANCER ACTIVITY OF CURCUMA XANTHORRHIZA FERMENTED JUICES

2018 ◽  
Vol 11 (8) ◽  
pp. 439
Author(s):  
Warintorn Ruksiriwanich ◽  
Hiroyuki Akazawa ◽  
Pensak Jantrawut
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Tyrosinase Inhibition ◽  
Normal Human Skin ◽  
Metal Chelating ◽  
Normal Human ◽  
Dpph Scavenging ◽  
Lipid Peroxidation Inhibition ◽  
Fermented Juice

Objective: The objective of this study was to investigate the anticancer activity and toxicity of fermented juices from six Thai plants, Garcinia mangostana Linn., Tinospora crispa Linn., Litchi chinensis Sonn., Dimocarpus longan Lour., Curcuma xanthorrhiza (CX) Roxb., and Averrhoa bilimbi Linn., in normal cells as compared with common commercial fermented juices.Methods: The plants were fermented for 3 months, and their biological activities including 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging, metal chelating, lipid peroxidation inhibition, and tyrosinase inhibition activities were subsequently evaluated. Furthermore, their in vitro anticancer activities in human colon adenocarcinoma (HT-29), cervical cancer (HeLa), hepatocellular carcinoma (HepG2), and murine melanoma (B16F10) cell lines and their toxicities in normal human skin fibroblasts were assessed.Results: The fermented juice of CX showed a higher antioxidant activity than those of the other plants, with an SC50 (DPPH scavenging) value of 0.011 mg/mL, an IPC50 (lipid peroxidation inhibition) of 0.027 mg/mL, an MC50 (metal chelating) value of 0.170 mg/mL, and an IC50 (tyrosinase inhibition) of 0.027 mg/mL. Moreover, the fermented juice of CX displayed selective toxicity in cancer cell lines (HeLa, HepG2, and B16F10), with a significantly lower toxicity in normal human skin fibroblasts as compared with an expensive commercial fermented juice product.Conclusion: The present study suggests that the fermented juice of CX can be developed as a functional food supplement with antioxidant properties or as an anticancer product with low toxicity to normal human skin fibroblasts.

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