Antifungal activity of photo-biosynthesized silver nanoparticles (AgNPs) from organic constituents in orange peel extract against phytopathogenic Macrophomina phaseolina

Author(s):  
Yasser Mahmoud A. Mohamed ◽  
Ibrahim E. Elshahawy
2014 ◽  
Vol 9 (3) ◽  
pp. 34-40 ◽  
Author(s):  
A. Awad Manal ◽  
A. Hendi Awatif ◽  
M. O. Ortashi Khalid ◽  
F. A. Elradi Dalia ◽  
E. Eisa Nada ◽  
...  

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2363
Author(s):  
Yasser S. Mostafa ◽  
Saad A. Alamri ◽  
Sulaiman A. Alrumman ◽  
Mohamed Hashem ◽  
Zakaria A. Baka

This study aimed to synthesize silver nanoparticles (AgNPs) by pomegranate and orange peel extracts using a low concentration of AgNO3 solution to controlearly blight of tomato caused by Alternaria solani. The pathogen was isolated from infected tomato plants growing in different areas of Saudi Arabia. The isolates of this pathogen were morphologically and molecularly identified. Extracts from peels of pomegranate and orange fruits effectively developed a simple, quick, eco-friendly and economical method through a synthesis of AgNPs as antifungal agents against A. solani. Phenolic content in the pomegranate peel extract was greater than orange peel extract. Phenolic compounds showed a variation of both peel extracts as identified and quantified by High-Performance Liquid Chromatography. The phenolic composition displayed variability as the pomegranate peel extract exhibited an exorbitant amount of Quercitrin (23.62 mg/g DW), while orange peel extract recorded a high amount of Chlorogenic acid (5.92 mg/g DW). Biosynthesized AgNPs were characterized using UV- visible spectroscopy which recorded an average wavelength of 437 nm and 450 nm for pomegranate and orange peels, respectively. Fourier-transform infrared spectroscopy exhibited 32x73.24, 2223.71, 2047.29 and 1972.46 cm−1, and 3260.70, 1634.62, 1376.62 and 1243.76 cm−1 for pomegranate and orange peels, respectively. Transmission electron microscopy showed spherical shape of nanoparticles. Zetasizer analysis presented negative charge values; −16.9 and −19.5 mV with average particle sizes 8 and 14 nm fin case of pomegranate and orange peels, respectively. In vitro, antifungal assay was done to estimate the possibility of biosynthesized AgNPs and crude extracts of fruit peels to reduce the mycelial growth of A. solani. AgNPs displayed more fungal mycelial inhibition than crude extracts of two peels and AgNO3. We recommend the use of AgNPs synthesized from fruit peels for controlling fungal plant pathogens and may be applied broadly and safely in place by using the chemical fungicides, which display high toxicity for humans.


2020 ◽  
Vol 14 (3) ◽  
pp. 250-258
Author(s):  
Jose E. Quiroz-Hernández ◽  
Oxana V. Kharissova ◽  
Victor E. Aguirre-Arzola ◽  
Guillermo C.G. Martinez-Avila ◽  
Uziel Castillo-Velazquez

Aims: To use an agroindustrial waste (orange peels) as a source of polyphenols as a reducing medium for obtaining silver nanoparticles by greener method. Background: Several techniques have been employed for AgNPs synthesis, nevertheless, most of them involve the use of toxic chemicals in the process. The use of fungi, bacteria, and plant extracts as subtracts for green synthesis is an ecofriendly alternative, although hypothetic, route for AgNPs large scale synthesis. In the case of plant extracts, it is believed that polyphenols are the biomolecules responsible for the reduction and stabilization of the Ag+ ions into AgNPs, being a sustainable and ecological option; polyphenols could be obtained from plant waste and agroindustrial subproducts. Objective: To develop an efficient, greener, and low-cost method of AgNPs production using natural products. Methods: The basic principle of silver nanoparticles synthesis is the interaction in a mixture of silver nitrate (source of Ag+ ions) and the orange peel extract (reducing and stabilizing agent) under certain conditions. Five treatments were carried out, evaluating several parameters during AgNPs synthesis such as pH, orange peel extract-silver nitrate ratio, time and conditions of incubation, irradiation of UV light, irradiation of microwave, and temperature. Result: The synthesis of silver nanoparticles from an agroindustrial waste as the orange peel was successfully carried out and checked by visual evaluation, UV-Vis spectroscopy, and EDS analysis. The particle size was estimated between 42.82 nm to 151.75 nm, having a spherical and ovoid morphology. Discussion: Through the analysis of several synthesis conditions, it has become possible to establish a suitable treatment to increase antibacterial yield and evaluate morphology and size traits in order to acquire the best conditions for a future industrial scale synthesis. Conclusion: The orange peel aqueous extract resulted as a great source of polyphenols, allowing the successful synthesis of silver nanoparticles in mild conditions. Thus, obtained AgNPs revealed an increased antibacterial effect and potential against Gram-positive bacteria such as Staphyloccocus aureus.


2013 ◽  
Vol 2 (3) ◽  
pp. 367-376 ◽  
Author(s):  
Genevieve A. Kahrilas ◽  
Laura M. Wally ◽  
Sarah J. Fredrick ◽  
Michael Hiskey ◽  
Amy L. Prieto ◽  
...  

2021 ◽  
Vol 19 (6) ◽  
pp. 1-13
Author(s):  
Cathleen Simatupang ◽  
◽  
Vinod K Jindal ◽  
Ranjna Jindal ◽  
◽  
...  

Interest in the biosynthesis of silver nanoparticles (AgNPs) has been steadily increasing primarily due to their numerous applications in various fields, low-cost, use of non-toxic environmentally-friendly materials and easy implementation. This study focused on the biosynthesis of AgNPs using orange peel extract (OPE), optimization of process conditions, and application in catalytic degradation of methylene blue (MB) dye used in the textile industry. A central composite design in response surface methodology resulted in optimum conditions of 0.0075 g dry peel/mL for OPE concentration, pH of 11 and 1.5 mM silver nitrate concentration. The optimum conditions for the response variables corresponded to the peak absorbance of 0.79 and SPR wavelength of 403.8 nm in UV-vis spectra, and minimum particle size of 12.9 nm. In addition, peak absorbance and SPR wavelength appeared to be related to the size of the AgNPs. A full-factorial design for the catalytic degradation of MB dye by the biosynthesized AgNPs for 1 h indicated the maximum influence of AgNPs compared to the concentrations of MB dye and NaBH4 in decreasing order. The MB dye was reduced rapidly with NaBH4 in the presence of AgNPs due to their catalytic action. The findings of the study show the potential of OPE for the biosynthesis of AgNPs with excellent catalytic activity for the treatment of MB dye in industrial effluent.


2016 ◽  
Vol 40 ◽  
pp. 288-297 ◽  
Author(s):  
Mehmet ÇİFTÇİ ◽  
Ülkü Gülcihan ŞİMŞEK ◽  
Bestami DALKILIÇ ◽  
Mehmet Ali AZMAN ◽  
Ökkeş YILMAZ ◽  
...  

2007 ◽  
Vol 846 (1-2) ◽  
pp. 291-297 ◽  
Author(s):  
Shiming Li ◽  
Ted Lambros ◽  
Zhenyu Wang ◽  
Robert Goodnow ◽  
Chi-Tang Ho

2016 ◽  
Vol 12 (3) ◽  
pp. 303-310 ◽  
Author(s):  
Omid Hasani ◽  
Seyed Rohollah Javadian

Abstract The aim of the present study was to evaluate antioxidative activities of the encapsulated and unencapsulated bitter orange peel extract on the shelf-life of the common carp fillets during 16 days of refrigerated storage. Common carp fillets were treated with bitter orange peel extract (0.5 and 1% w/w), liposomal encapsulated bitter orange peel extract (0.5 and 1% w/w) and beta-hydroxytoluene (BHT, 100 and 200 ppm); their quality changes in terms of total volatile basic nitrogen (TVB-N), free fatty acid (FFA) peroxide value (PV), thiobarbituric acid (TBA) and sensory characteristics were investigated. Results showed that the encapsulated and pure extract and BHT could reduce chemical deterioration and lipid oxidation in the fillets compared to the control, as reflected with lower TVBN, FFA, PV and TBA values. Furthermore, common carp fillets treated with high concentration (1%) of the encapsulated bitter orange peel extract showed the lowest amount of lipid oxidation during the storage period compared with the others without any undesirable changes in sensorial attributes.


2012 ◽  
Vol 111 (4) ◽  
pp. 1757-1769 ◽  
Author(s):  
Kadarkarai Murugan ◽  
Palanisamy Mahesh Kumar ◽  
Kalimuthu Kovendan ◽  
Duraisamy Amerasan ◽  
Jayapal Subrmaniam ◽  
...  

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