Molecular detection and prevalence of Theileria ovis and Anaplasma marginale in sheep blood samples collected from Layyah district in Punjab, Pakistan

2021 ◽  
Vol 53 (4) ◽  
Author(s):  
Kainat Abid ◽  
Sundas Bukhari ◽  
Muhammad Asif ◽  
Ayesha Sattar ◽  
Maryam Arshad ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Anaplasma Marginale ◽  
Blood Samples ◽  
Sheep Blood

A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)

2020 ◽  
Author(s):  
Sehrish Ashraf ◽  
Asia Parveen ◽  
Mian Muhammad Awais ◽  
Quratulane Gillani ◽  
Munir Aktas ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Anaplasma Marginale ◽  
Blood Samples

First Report Regarding the Simultaneous Molecular Detection of Anaplasma marginale and Theileria annulata in Equine Blood Samples Collected from Southern Punjab in Pakistan

2019 ◽  
Vol 65 (1) ◽  
pp. 259-263
Author(s):  
Muhammad Asif ◽  
Asia Parveen ◽  
Sehrish Ashraf ◽  
Mureed Hussain ◽  
Munir Aktas ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Anaplasma Marginale ◽  
Theileria Annulata ◽  
Blood Samples ◽  
First Report

Molecular detection of Anaplasma marginale infection in carrier cattle

2012 ◽  
Vol 3 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Harkirat Singh ◽  
Jyoti ◽  
Manjurul Haque ◽  
Nirbhay K. Singh ◽  
Shitanshu S. Rath
Keyword(s):  
Molecular Detection ◽  
Anaplasma Marginale

scholarly journals Epizootic Situation on Anaplasmosis of Small Ruminants in the Irkutsk Region

2021 ◽  
Vol 6 (1) ◽  
pp. 60-68
Author(s):  
O. V. Suntsova ◽  
V. A. Rar ◽  
O. V. Lisak ◽  
I. V. Meltsov ◽  
E. K. Doroschenko ◽  
...  
Keyword(s):  
Nucleotide Substitution ◽  
Small Ruminants ◽  
Wide Distribution ◽  
Anaplasma Ovis ◽  
Single Nucleotide ◽  
Blood Samples ◽  
Sheep Blood ◽  
Irkutsk Region ◽  
Probable Role

Anaplasmosis of ruminants is a group of natural focal infections caused by bacteria from the genus Anaplasma of the Anaplasmataceae family. The main etiological agent of anaplasmosis in sheep, goats, and wild ruminants is Anaplasma ovis, which parasitizes in the erythrocytes of these animals. The purpose of this study was the finding and identification of Anaplasma spp. in the blood of small ruminants using genetic methods and obtaining data on the distribution of anaplasmosis in the Irkutsk region. 20 goat blood samples, 611 sheep blood samples and 209 Dermacentor nuttalli ticks from 12 districts of the Irkutsk region were examined for the presence of Anaplasma spp. Only one type of anaplasma, A. ovis, was found among the genotyped samples. A. ovis was found in the blood of sheep and goats in all of the studied districts of the Irkutsk region. The proportion of sheep blood samples containing anaplasma DNA varied from 30 % to 85 %, in goats – from 10 % to 100 % in different districts, and averaged 57.8 % in sheep and 55,0 % in goats. Frequency of infection of D. nuttalli ticks with A. ovis was 5.7 %. The nucleotide sequences of the samples detected in the blood of small ruminants on the territory of the Irkutsk region differed from each other by a single nucleotide substitution and were identical to the sequences of the type strain Haibei, as well as the sequences of A. ovis previously found in the blood of sheep from Mongolia, deer from China, and Dermacentor niveus and Dermacentor nuttalli ticks from China. These sequences were also identical to the sequences previously found in the blood of sheep from Altai and in Dermacentor nuttalli ticks from Tuva, which indicates the wide distribution of these A. ovis genovariants in Siberia and the probable role of D. nuttalli as a carrier of the agent of anaplasmosis of small ruminants in the Irkutsk region.

scholarly journals Molecular detection and occurrence of equine theileriosis in Arabian horses in Al-Najaf province/Iraq

2020 ◽  
Vol 57 (3) ◽  
pp. e166996
Author(s):  
Hayder Mohammad Al-Rammahi ◽  
Abdulameer Abed Hatem ◽  
Asaad Chasib Al-Atabi
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Molecular Detection ◽  
Molecular Technique ◽  
Chain Reaction ◽  
Blood Samples ◽  
Equine Piroplasmosis ◽  
Polymerase Chain

This study was designed to detect equine piroplasmosis using the molecular technique in Al-Najaf province during the season that showed an increment in tick activities. Blood samples were collected from 110 horses with more than two signs of piroplasmosis. After DNA extraction, the product was examined by a polymerase chain reaction to amplify 18SrRNA. The results showed that the overall percentage of equine theileriosis was 38.18%. According to gender, the percentage of infection was 43.48% and 29.27% in females and males, respectively. Significant variations appeared between infected horses according to age, and the percentage of infection was 50% and 35.22% in less than 2 years and more than 2 years age, respectively. Moreover, the percentage of infection was 62.5% and 19.35% in animals with and without acariasis, respectively. Significant variations were also seen in equine theileriosis according to geographical areas, and the higher percentage was reported in Hera district (60.87%), while the lowest percentage was in the center of Al-Najaf (21.43%). This difference may be due to different distribution of vector of disease (tick), which may be the availability of the suitable weather that helped in the multiplication of the intermediate vectors. In conclusion, this study proved the variations in the occurrences of equine piroplasmosis according to gender, age, and geographical areas.

scholarly journals Selenium and other mineral concentrations in feed and sheep's blood in Kosovo

2017 ◽  
Vol 1 (1) ◽  
pp. 97-107 ◽  
Author(s):  
A. Ademi ◽  
A. Bernhoft ◽  
E. Govasmark ◽  
H. Bytyqi ◽  
T. Sivertsen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Whole Blood ◽  
Inductively Coupled Plasma ◽  
Blood Samples ◽  
Sheep Blood ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Toxic Minerals ◽  
Mineral Concentrations ◽  
Feed Samples

Abstract The aim of this study was to assess the concentration of Se and other minerals in sheep and the supplied feed. Four macrominerals (Ca, P, Mg, and S), 7 microminerals (Se, Fe, Zn, Cu, Mn, Co, and Mo), and 2 toxic minerals (Cd and Pb) were analyzed in 69 feed and 292 sheep blood samples from 30 farms in different regions of Kosovo. The samples were analyzed using inductively coupled plasma mass spectrometry, and mineral concentrations in whole blood were measured to assess their status in animals. Concentrations of the different minerals in feed were found in the following ranges: 1.9 to 9.5 g Ca/kg DM, 0.8 to 3.2 g P/kg DM, 0.8 to 3.2 g Mg/kg DM, 1.0 to 2.8 g S/kg DM, 6 to 82 µg Se/kg DM, 33 to 970 mg Fe/kg DM, 15 to 42 mg Zn/ kg DM, 2.6 to 7.5 mg Cu/kg DM, 26 to 250 mg Mn/kg DM, 0.04 to 0.88 mg Co/kg DM, 0.05 to 0.86 mg Mo/ kg DM, 0.07 to 2.02 mg Pb/kg DM, and 0.02 to 0.19 mg Cd/kg DM. Concentrations of the microminerals analyzed in whole blood were found in the following ranges: 15 to 360 µg Se/L, 190 to 500 mg Fe/L, 1.4 to 3.8 mg Zn/L, 0.3 to 2.6 mg Cu/L, 6 to 243 µg Mn/L, 0.1 to 19.6 µg Co/L, and 1.8 to 66.0 µg Pb/L. Among all minerals, the largest deficiency was found for Se both in feed and sheep blood, with 82% of feed samples and 83% blood samples being inadequate, and its supplementation is necessary. Selenium-supplemented sheep had significantly higher Se concentration in blood than non-supplemented sheep (P < 0.01). In addition, other macro- and microminerals in feed such as P, S, Cu, and Co were at inadequate concentrations at some of the farms, and supplementation may also be needed for these minerals.

scholarly journals Molecular Analysis of Canine Filaria and Its Wolbachia Endosymbionts in Domestic Dogs Collected from Two Animal University Hospitals in Bangkok Metropolitan Region, Thailand

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 114 ◽  
Author(s):  
Hathaithip Satjawongvanit ◽  
Atchara Phumee ◽  
Sonthaya Tiawsirisup ◽  
Sivapong Sungpradit ◽  
Narisa Brownell ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Neglected Tropical Diseases ◽  
Control Strategies ◽  
Domestic Dogs ◽  
Metropolitan Region ◽  
World Health ◽  
University Hospitals ◽  
Effective Prevention ◽  
Blood Samples ◽  
Filarial Nematodes

Canine filariasis is caused by several nematode species, such as Dirofilaria immitis, Dirofilaria repens, Brugia pahangi, Brugia malayi, and Acanthocheilonema reconditum. Zoonotic filariasis is one of the world’s neglected tropical diseases. Since 2000, the World Health Organization (WHO) has promoted a global filarial eradication program to eliminate filariasis by 2020. Apart from vector control strategies, the infection control of reservoir hosts is necessary for more effective filariasis control. In addition, many studies have reported that Wolbachia is necessary for the development, reproduction, and survival of the filarial nematode. Consequently, the use of antibiotics to kill Wolbachia in nematodes has now become an alternative strategy to control filariasis. Previously, a case of subconjunctival dirofilariasis caused by Dirofilaria spp. has been reported in a woman who resides in the center of Bangkok, Thailand. Therefore, our study aimed to principally demonstrate the presence of filarial nematodes and Wolbachia bacteria in blood collected from domestic dogs from the Bangkok Metropolitan Region, Thailand. A total of 57 blood samples from dogs with suspected dirofilariasis who had visited veterinary clinics in Bangkok were collected. The investigations for the presence of microfilaria were carried out by using both microscopic and molecular examinations. PCR was used as the molecular detection method for the filarial nematodes based on the COI and ITS1 regions. The demonstration of Wolbachia was performed using PCR to amplify the FtsZ gene. All positive samples by PCR were then cloned and sequenced. The results showed that the filarial nematodes were detected in 16 samples (28.07%) using microscopic examinations. The molecular detection of filarial species using COI-PCR revealed that 50 samples (87.72%) were positive; these consisted of 33 (57.89%), 13 (22.81%), and 4 (7.02%) samples for D. immitis, B. pahangi, and B. malayi, respectively. While the ITS1-PCR showed that 41 samples (71.93%) were positive—30 samples (52.63%) were identified as containing D. immitis and 11 samples (19.30%) were identified to have B. pahangi, whereas B. malayi was not detected. Forty-seven samples (82.45%) were positive for Wolbachia DNA and the phylogenetic tree of all positive Wolbachia was classified into the supergroup C clade. This study has established fundamental data on filariasis associated with Wolbachia infection in domestic dogs in the Bangkok Metropolitan Region. An extensive survey of dog blood samples would provide valuable epidemiologic data on potential zoonotic filariasis in Thailand. In addition, this information could be used for the future development of more effective prevention and control strategies for canine filariasis in Thailand.

scholarly journals Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Natasha Gandolfi Miceli ◽  
Fernando Antonio Gavioli ◽  
Luiz Ricardo Gonçalves ◽  
Marcos Rogério André ◽  
Valéria Régia Franco Sousa ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Western Region ◽  
Mato Grosso ◽  
Animal Shelters ◽  
Blood Samples ◽  
Cytauxzoon Felis ◽  
The City ◽  
Veterinary Hospital

Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) forMycoplasma haemofelis, 12 (6.7%) for ‘Candidatus M. haemominutum’ and one (0.5%) for ‘Candidatus M. turicensis’. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

Revisiting the Tams1-encoding gene as a species-specific target for the molecular detection of Theileria annulata in bovine blood samples

2013 ◽  
Vol 4 (1-2) ◽  
pp. 72-77 ◽  
Author(s):  
Marcos Santos ◽  
Ricardo Soares ◽  
Pedro Costa ◽  
Ana Amaro ◽  
João Inácio ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Theileria Annulata ◽  
Bovine Blood ◽  
Blood Samples ◽  
Specific Target ◽  
Encoding Gene ◽  
Species Specific

Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Handan Cetinkaya ◽  
Damla Haktanir ◽  
Seckin Arun ◽  
Cem Vurusaner
Keyword(s):  
Molecular Detection ◽  
Fragment Length Polymorphism ◽  
Common Species ◽  
Mycoplasma Species ◽  
Pcr Assay ◽  
Blood Samples ◽  
Total Prevalence ◽  
Polymerase Chain ◽  
First Time ◽  
Candidatus Mycoplasma Turicensis

AbstractThe aim of this study was to investigate Mycoplasma spp. species in blood samples of the domestic cats from the province of Istanbul, Turkey. Three hundred eighty four blood samples of client-owned cats were used for the identification of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm) and Candidatus Mycoplasma turicensis (CMt) by Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) assays. Out of 384 blood samples, 74 (19.3%) were positive for one of Mycoplasma species. The total prevalence of Mhf, CMhm and CMt infections was 9.9%, 17.7% and 0.8% respectively. The most common species was CMhm. Co-infections were mostly with Mhf/CMhm and the frequency was 8.1%. Two cats were infected with three species. The current study was the first molecular prevalence study of hemotropic mycoplasmas in Istanbul, reporting the presence of CMt for the first time in Turkey. Prevalence of feline mycoplasma was notably high in Istanbul and PCR assay could be preferred rather than the microscopic examination for the diagnosis.

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