Molecular detection of Anaplasma marginale infection in carrier cattle

2012 ◽  
Vol 3 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Harkirat Singh ◽  
Jyoti ◽  
Manjurul Haque ◽  
Nirbhay K. Singh ◽  
Shitanshu S. Rath
2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Carlos Ramón Bautista Garfias ◽  
Tania Rodríguez ◽  
Carmen Rojas ◽  
José J. Lira ◽  
Jesús A. Álvarez ◽  
...  

Data on the implication of the stable fly (Stomoxys calcitrans, Diptera: Mus­cidae), in the transmission of Anaplasma marginale in tick-free cattle is scarce. Hence, the objective of this investigation was to detect the presence of DNA from A. marginale in stable flies caught near a bovine herd, which has been maintained free of ticks for 40 years, and occasionally presents some clinical cases of anaplasmosis. Twenty-four batches of S. calcitrans (15 flies each) were collected in the morning and in the afternoon twice a week (except for one catch in one week of September and one catch in one week of December) during 12 catching days in a period of four months. Their DNA was obtained and analysed by nested PCR (nPCR) to identify the presence of A. marginale DNA. Seven of the batches (29.16 %) were pos­itive for A. marginale, as detected by an nPCR that targets the A. marginale msp5 gene. Similarly, in two out of 12 catching days (16.66 %), those S. calcitrans batches collected in the morning and in the afternoon, were pos­itive for A. marginale, while those S. calcitrans groups collected in three out of 12 catching days (25 %), were positive for the rickettsia. The obtained re­sults suggest that A. marginale transmission is carried out mechanically by S. calcitrans, favouring the circulation and maintenance of the microorganism in this particular bovine herd.


2020 ◽  
Vol 65 (3) ◽  
pp. 620-627
Author(s):  
Khaled Mohamed El-Dakhly ◽  
Waleed M. Arafa ◽  
Saad Soliman ◽  
Omima Ramadan Abdel-Fatah ◽  
Ahmed Anwar Wahba ◽  
...  

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Paul Franck Adjou Moumouni ◽  
Gabriel Oluga Aboge ◽  
Mohamad Alaa Terkawi ◽  
Tatsunori Masatani ◽  
Shinuo Cao ◽  
...  

2016 ◽  
Vol 116 (2) ◽  
pp. 751-762 ◽  
Author(s):  
Charoonluk Jirapattharasate ◽  
Paul Franck Adjou Moumouni ◽  
Shinuo Cao ◽  
Aiko Iguchi ◽  
Mingming Liu ◽  
...  

2018 ◽  
Vol 9 (3) ◽  
pp. 580-588 ◽  
Author(s):  
Charles Byaruhanga ◽  
Nicola E. Collins ◽  
Darryn L. Knobel ◽  
Zamantungwa T.H. Khumalo ◽  
Mamohale E. Chaisi ◽  
...  

Author(s):  
Emad Omer ◽  
Mohammed Elfehid ◽  
Abdelatiff Alwazan ◽  
Abdulaziz S. Alouffi ◽  
Fahdah A. Alshammari ◽  
...  

Acta Tropica ◽  
2020 ◽  
Vol 205 ◽  
pp. 105338 ◽  
Author(s):  
Witchuta Junsiri ◽  
Amaya Watthanadirek ◽  
Napassorn Poolsawat ◽  
Sarawan Kaewmongkol ◽  
Sathaporn Jittapalapong ◽  
...  

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