Preparation and investigation of Ulex europaeus agglutinin I-conjugated liposomes as potential oral vaccine carriers

2011 ◽  
Vol 34 (11) ◽  
pp. 1899-1907 ◽  
Author(s):  
KeXin Li ◽  
DaWei Chen ◽  
XiuLi Zhao ◽  
HaiYang Hu ◽  
ChunRong Yang ◽  
...  
Keyword(s):  
Oral Vaccine ◽  
Ulex Europaeus ◽  
Ulex Europaeus Agglutinin I

scholarly journals Application of Ulex europaeus Agglutinin I-Modified Liposomes for Oral Vaccine: Ex Vivo Bioadhesion and in Vivo Immunity

2011 ◽  
Vol 59 (5) ◽  
pp. 618-623 ◽  
Author(s):  
KeXin Li ◽  
XiuLi Zhao ◽  
ShiYi Xu ◽  
DaHai Pang ◽  
ChunRong Yang ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Oral Vaccine ◽  
Ulex Europaeus ◽  
Ulex Europaeus Agglutinin I

scholarly journals Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate–Lectin Interactions by QCM Biosensor

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1275 ◽  
Author(s):  
Kun Shang ◽  
Siyu Song ◽  
Yaping Cheng ◽  
Lili Guo ◽  
Yuxin Pei ◽  
...  
Keyword(s):  
Real Time ◽  
Con A ◽  
Quartz Crystals ◽  
Ulex Europaeus ◽  
Novel Approach ◽  
Ulex Europaeus Agglutinin I ◽  
Flow Through ◽  
Time Determination ◽  
Kinetics Of

A novel approach for preparing carbohydrate chips based on polydopamine (PDA) surface to study carbohydrate–lectin interactions by quartz crystal microbalance (QCM) biosensor instrument has been developed. The amino-carbohydrates were immobilized on PDA-coated quartz crystals via Schiff base reaction and/or Michael addition reaction. The resulting carbohydrate-chips were applied to QCM biosensor instrument with flow-through system for real-time detection of lectin–carbohydrate interactions. A series of plant lectins, including wheat germ agglutinin (WGA), concanavalin A (Con A), Ulex europaeus agglutinin I (UEA-I), soybean agglutinin (SBA), and peanut agglutinin (PNA), were evaluated for the binding to different kinds of carbohydrate chips. Clearly, the results show that the predicted lectin selectively binds to the carbohydrates, which demonstrates the applicability of the approach. Furthermore, the kinetics of the interactions between Con A and mannose, WGA and N-Acetylglucosamine were studied, respectively. This study provides an efficient approach to preparing carbohydrate chips based on PDA for the lectin–carbohydrate interactions study.

Abstract 1945: Levels Of Circulating Progenitor Cells In Patients With Chronic Heart Failure Predict Outcomes

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Roberto Latini ◽  
Ralph Lehmann ◽  
Birgit Assmus ◽  
Giovanna Balconi ◽  
Stefanie Dimmeler ◽  
...  
Keyword(s):  
Heart Failure ◽  
Progenitor Cells ◽  
Mononuclear Cells ◽  
Nyha Class ◽  
Double Positive ◽  
Ulex Europaeus ◽  
Vascular Regeneration ◽  
Circulating Endothelial Progenitor Cells ◽  
Cellular Marker ◽  
Ulex Europaeus Agglutinin I

Background: A low level of circulating endothelial progenitor cells (EPCs), a marker of reduced vascular regeneration, is related to worse outcome in patients with coronary heart disease, but little is known on pts with heart failure (HF). The aim was to assess the prognostic value of circulating EPCs in patients with HF. Methods: In 68 pts enrolled to GISSI-HF trial by 5 Italian Centers and 45 pts from Frankfurt, mononuclear cells from peripheral blood were isolated and plated; after 4 days, adherent cells were incubated with DiI-labeled acetylated LDL, stained with FITC-labeled Ulex europaeus agglutinin I (lectin), and counted as double positive at confocal microscopy. 66 patients had ischemic etiology, 12 were females, 26 had NYHA class >II, median age 64 y (Q1–Q3 56–70y). Cutoffs for EPCs were assessed by ROC. Results: Mean EPCs in pts with HF 34 ± 7 EPCs/mm 2 , 2–3 fold lower than in age-matched controls. Over a median of 2.5 years, 12 CV deaths and 47 first hospitalizations for CV reasons were recorded. Levels of EPCs were unrelated to severity of HF, age or sex. After adjustment for clinical variables, creatinine and BNP, EPCs <30/mm 2 were associated with a 2-fold higher risk of CV death and hospitalization, as shown by K-M curves (figure ) and by Cox multivariate regression. Pts with HF of ischemic etiology gave identical results. Conclusions : The level of circulating EPCs is an independent predictor of CV death and hospitalization in pts with chronic HF. The assay of this cellular marker of risk can be performed in blood samples collected in a multicenter setting under controlled conditions, and may offer an accessible tool to assess the role of vascular regeneration in the treatment of HF.

Sarcomatoid Collecting Duct Carcinoma

1999 ◽  
Vol 123 (4) ◽  
pp. 338-341 ◽  
Author(s):  
Masaharu Fukunaga
Keyword(s):  
Collecting Duct ◽  
Fatal Case ◽  
Flow Cytometric Analysis ◽  
Cytokeratin 19 ◽  
Duct Carcinoma ◽  
Collecting Duct Carcinoma ◽  
Ulex Europaeus ◽  
Atypical Cells ◽  
Ulex Europaeus Agglutinin I ◽  
Desmoplastic Stroma

Abstract Background.—Collecting duct carcinoma has recently been recognized as a distinct entity among renal cell carcinomas and shows aggressive clinical behavior. Design.—A fatal case of collecting duct carcinoma with sarcomatoid features in an 80-year-old man is described, and the results of immunohistochemistry and flow cytometric analysis are presented. Results.—The tumor, which was white and had infiltrating margins in the cortex and medulla, consisted of adenocarcinomatous and sarcomatous components. The adenocarcinomatous component was characterized by tubular arrangement of atypical cells with high-grade nuclear features and desmoplastic stroma. The sarcomatous component had a malignant fibrous histocytoma-like appearance. The tumor cells in the adenocarcinomatous area were positive for cytokeratin 19, Ulex europaeus agglutinin I, peanut agglutinin, and soybean agglutinin. The tumor was DNA diploid. The patient died of disease 3 months after diagnosis. Conclusion.—This study suggests that sarcomatoid collecting duct carcinoma may be one of the most aggressive tumors of the kidney.

scholarly journals A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor

Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1023
Author(s):  
Lili Guo ◽  
Shuang Chao ◽  
Pei Huang ◽  
Xiukai Lv ◽  
Quanquan Song ◽  
...  
Keyword(s):  
Schiff Base ◽  
Quartz Crystal Microbalance ◽  
Concanavalin A ◽  
Quartz Crystal ◽  
Sensor Surface ◽  
Con A ◽  
Ulex Europaeus ◽  
Plant Lectins ◽  
Photochemical Method ◽  
Ulex Europaeus Agglutinin I

A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via Schiff base reactions. Upon light irradiation, the underivatized carbohydrates were inserted into the sensor surface, including mannose, galactose, fucose and N-acetylglucosamine (GlcNAc). Carbohydrate sensors were evaluated for the binding to a series of plant lectins. A kinetic study of the interactions between mannose and concanavalin A (Con A), fucose and Ulex europaeus agglutinin I (UEA-I) were performed. This method can eliminate the tedious modification of carbohydrates, improve the experimental efficiency, and reduce the experimental cost, which is of great significance for the development of QCM biosensors and the study of biomolecular interactions.

scholarly journals Angiogenesis in Pituitary Adenomas and the Normal Pituitary Gland

2000 ◽  
Vol 85 (3) ◽  
pp. 1159-1162 ◽  
Author(s):  
Helen E. Turner ◽  
Zsusha Nagy ◽  
Kevin C. Gatter ◽  
Margaret M. Esiri ◽  
Adrian L. Harris ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Pituitary Tumors ◽  
Anterior Pituitary Gland ◽  
Ulex Europaeus ◽  
Normal Pituitary Gland ◽  
Ulex Europaeus Agglutinin I ◽  
Acth Secreting ◽  
Tumor Types

Abstract Angiogenesis is essential for tumor growth beyond a few millimeters in diameter, and the intratumoral microvessel count that represents a measure of angiogenesis has been correlated with tumor behavior in a variety of different tumor types. To date no systematic study has assessed pituitary tumors of different secretory types, correlating vascular count with tumor size. The vascular densities of pituitary tumors and normal anterior pituitary were therefore assessed by counting vessels labeled using the vascular markers CD31 and ulex europaeus agglutinin I. One hundred and twelve surgically removed pituitary adenomas (30 GH-secreting, 25 prolactinomas, 15 ACTH-secreting, and 42 nonfunctioning tumors) were compared with 13 specimens of normal anterior pituitary gland. The vascular counts in the normal anterior pituitary gland were significantly higher (P &lt; 0.05) than those in the tumors using both CD31 and ulex europaeus agglutinin I. In addition, microprolactinomas were significantly less vascular (P &lt; 0.05) than macroprolactinomas, although there was no such difference between vascular densities of microadenomas and macroadenomas producing GH. ACTH-secreting tumors were, like microprolactinomas, of much lower vascular density than the normal pituitary and other secreting and nonsecreting tumor types. In marked contrast to other tumors, pituitary adenomas are less vascular than the normal pituitary gland, suggesting that there may be inhibitors of angiogenesis that play an important role in the behavior of these tumors.

Transgene vaccination using Ulex europaeus agglutinin I (UEA-1) for targeted mucosal immunization against HIV-1 envelope

Vaccine ◽  
2005 ◽  
Vol 23 (29) ◽  
pp. 3836-3842 ◽  
Author(s):  
Xinhai Wang ◽  
Irina Kochetkova ◽  
Asmahan Haddad ◽  
Teri Hoyt ◽  
David M. Hone ◽  
...  
Keyword(s):  
Mucosal Immunization ◽  
Ulex Europaeus ◽  
Ulex Europaeus Agglutinin I ◽  
Hiv 1
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