Mutagenesis of a Thermophilic Alkalibacillus flavidus for Enhanced Production of an Extracellular Acetyl Xylan Esterase in Semi-solid Culture of Linseed Meal

2019 ◽  
Vol 11 (7) ◽  
pp. 3327-3335
Author(s):  
Sikander Ali ◽  
Saba Mahmood
Keyword(s):  
Linseed Meal ◽  
Acetyl Xylan Esterase ◽  
Solid Culture ◽  
Enhanced Production ◽  
Semi Solid

scholarly journals Preparation and immobilization Glucoamylase and Pectinase by CLEA method

2014 ◽  
Vol 17 (2) ◽  
pp. 45-51
Author(s):  
Giang Thi Linh Tran ◽  
Oanh Ngoc Huynh
Keyword(s):  
Aspergillus Niger ◽  
Enzyme Preparation ◽  
Immobilized Enzyme ◽  
Solution Culture ◽  
Crude Enzyme ◽  
Cross Linking ◽  
Solid Culture ◽  
Enzyme Solution ◽  
Crude Enzyme Solution ◽  
Semi Solid

CLEA method (cross-linking enzyme aggregates) combines enzyme preparation and immobilization from solution culture into the same step. In this study, we applied CLEA method to immobilize two enzymes, glucoamylase and pectinase, from crude enzyme solution obtained from semi-solid culture of Aspergillus niger. The results showed that: In the same immobilized conditions (glucoamylase: 7% glutaraldehyde, 5°C, 2 hours; pectinase: 10% glutaraldehyde, 5°C, 2 hours), the immobilized enzyme from crude enzyme solution, has the abilities to be reused and activation stability under the influences of pH and temperature higher than immobilized commercial enzyme respectively.

scholarly journals Production of Trametes pubescens Laccase under Submerged and Semi-Solid Culture Conditions on Agro-Industrial Wastes

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73721 ◽  
Author(s):  
Juan C. Gonzalez ◽  
Sandra C. Medina ◽  
Alexander Rodriguez ◽  
Johann F. Osma ◽  
Carlos J. Alméciga-Díaz ◽  
...  
Keyword(s):  
Culture Conditions ◽  
Industrial Wastes ◽  
Solid Culture ◽  
Semi Solid

scholarly journals Optimization of an Efficient Semi-Solid Culture Protocol for Sterilization and Plant Regeneration of Centella asiatica (L.) as a Medicinal Herb

Molecules ◽  
2011 ◽  
Vol 16 (11) ◽  
pp. 8981-8991 ◽  
Author(s):  
Sina Siavash Moghaddam ◽  
Hawa Binti Jaafar ◽  
Maheran Abdul Aziz ◽  
Rusli Ibrahim ◽  
Asmah Bt Rahmat ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Centella Asiatica ◽  
Medicinal Herb ◽  
Solid Culture ◽  
Semi Solid ◽  
Culture Protocol

scholarly journals Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1010
Author(s):  
Eucario Mancilla-Álvarez ◽  
Juan Antonio Pérez-Sato ◽  
Rosalía Núñez-Pastrana ◽  
José L. Spinoso-Castillo ◽  
Jericó J. Bello-Bello
Keyword(s):  
Culture Medium ◽  
Solid Medium ◽  
Survival Rates ◽  
Shoot Multiplication ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Semi Solid

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

scholarly journals Investigation of the spore production of Paecilomyces spp. isolated from several agricultural soils with the biocontrol activily against Spodoptera litura

2018 ◽  
Vol 1 (T5) ◽  
pp. 58-68
Author(s):  
Linh Quoc Nguyen ◽  
Nhut Nhu Nguyen
Keyword(s):  
Spodoptera Litura ◽  
Solid Medium ◽  
Agricultural Soils ◽  
Management Strategies ◽  
Solid Culture ◽  
Biocontrol Activity ◽  
Semi Solid ◽  
Main Components ◽  
Native Strains

Paecilomyces is a fungus that parasites on various insect species. However, Paecilomyces has not been widely studied and applied in Vietnam. In this study, Paecilomyces spp. were isolated from several agricultural soils and identified based on the morphology and 28S rDNA gene sequencing. Biocontrol activities of Paecilomyces were measured in vitro against Spodoptera litura. The Paecilomyces strains with high biocontrol were studied for the spore acquisition on semi-solid culture. There were five isolated strains belonged to Paecilomyces (strain F01 belonged to P. javanicus, strain F02 belonged to Paecilomyces sp., strain F03 belonged to P. lilacinum and strains F04 and F05 belonged to P. lilacinus) from 33 different samples. In particular, both of F03 and F04 performed high biocontrol activity against S. litura after 10 days of inoculation. Optimization of spores production medium showed that F03 and F04 grew well on a defined semi-solid medium whose the main components were unpolished rice, wheat bran and husk of 55% humidity. The results indicated that the native strains of Paecilomyces were potential for applications to produce bioproducts for pest management strategies.

Disruption Of Ikaros In Patients’ Chronic Phase CML Cells Induces The Features Of Accelerated Phase Disease

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 513-513
Author(s):  
Philip A Beer ◽  
Ivan Sloma ◽  
Paul H Miller ◽  
David JHF Knapp ◽  
Gabrielle Rabu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chronic Phase ◽  
Genetic Alterations ◽  
Fluorescent Intensity ◽  
Qrt Pcr ◽  
Enhanced Production ◽  
Solid Media ◽  
Cd34 Cells ◽  
Semi Solid ◽  
Nonadherent Cells

Abstract Accelerated phase (AP) CML is marked by a rising leucocyte count in the face of maintenance treatment, exaggerated basophilia and accumulating blasts. However, AP is typically short-lived and rapidly succeeded by blast crisis. Genetic alterations resulting in loss of IKAROS activity are associated with the development of both BCR-ABL1+ myeloid and lymphoid leukemias, as well as the related BCR-ABL1-negative MPNs. However, how loss of IKAROS functionality contributes to the malignant features of these leukemias is not clear. Here we demonstrate that direct suppression of IKAROS activity in CD34+ cells from chronic phase (CP) CML patients leads to their rapid acquisition of features of AP without causing progression to acute leukemia. To suppress IKAROS activity in CD34+ CP CML cells, we transduced samples from 4 patients with a lentiviral GFP vector encoding a dominant-negative isoform of IKAROS (dnIK6), or an empty control YFP vector, and compared their progeny generated in cultures containing mouse stromal cells engineered to produce human SCF, IL-3, FLT3L and G-CSF. In all cases, we found dnIK6 transduction caused a rapid (evident within 2 weeks) and sustained (for at least 6 weeks) increase in total cell output (4-20x higher than YFP+ controls at 4-6 weeks, p<0.01). More detailed analysis showed that both populations of transduced cells produced similar numbers of monocytes and neutrophils and the increased cell output from the dnIK6-transduced cells was largely due to a consistent 50-200x increase in basophil production (with occasional increased eosinophils also), quantified using stained cytospins, and confirmed by flow cytometry (presence of CD34-33+117-14-15-HLA-DR-25+9+ cells). Similarly, comparison by qRT-PCR of dnIK6 versus control nonadherent cells present after 2-4 weeks showed 10-50-fold lower MPO, 2-9-fold higher HDC and 20-65-fold higher PRG2 transcripts. Notably, the cloning efficiency of the initially transduced control and dnIK6-transduced CD34+ CML cells in both semi-solid media and suspension culture was not significantly different. However, the proportion of dying/dead mature (nonadherent) cells (as measured by propidium iodide staining) derived from control cells was ∼30x higher than from the dnIK6-transduced cells (4.9±1.0% vs 0.15±0.03%, p=0.002). This suggested a dnIK6-augmented survival of terminally differentiating cells causing an accumulation of their mature progeny. In addition, assessment of 6-week cultures revealed a strikingly higher number of dnIK6-derived CD34+ cells and colony-forming cells as compared to controls (49±15-fold and 19±6-fold higher, respectively). To interrogate the molecular mechanisms underlying the dnIK6 effects on cell output, we compared the levels of activated signaling proteins by intracellular flow cytometry. This showed increased activation of STAT5 (80±14% higher median fluorescent intensity (MFI), p<0.001), MAPK (61±11% higher MFI, p=0.01) and β-catenin (44±9% higher MFI, p=0.02) in the CD34+ progeny of dnIK6-transduced CP CML cells. In addition, qRT-PCR analysis showed transcripts encoding key repressors of JAK-STAT (SOCS2, SH2B3) and β-catenin signaling (including GSK3B, AXIN1 and CK1) were suppressed in dnIK6-transduced CD34+ cells by comparison to CD34+ derivatives of control-transduced cells from the same patients. After a total of 12 weeks in culture, the control-transduced CML cells had largely disappeared, whereas the population produced by the dnIK6-transduced cells continued to expand, with a mean cumulative cell output after 12 weeks of 6x106 cells from 104 starting CD34+ cells (vs 2x105 from controls) along with a marked increase in CD34+ cells, rising from 1-3% at week 6, to 12-60% at week 12. However, plating of these CD34+ cells in semi-solid media yielded basophil or monocyte but not blast colonies, demonstrating that differentiation was not strongly impaired. In summary, IKAROS disruption in patients’ CP CD34+ CML cells recapitulates cardinal features of AP disease, including an increased cell output associated with an enhanced production of basophils and an eventual accumulation of primitive cells. We further identify dnIK6-mediated suppression of SOCS2 and the β-catenin destruction complex, with enhanced activation of STAT5 and β-catenin in progenitor cells, as a likely key mechanism contributing to the altered biology of AP cells. Disclosures: No relevant conflicts of interest to declare.

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