Human erythrocyte membrane fluidity and insulin binding are independent of dietary trans fatty acids

1994 ◽  
Vol 5 (12) ◽  
pp. 591-598 ◽  
Author(s):  
Elliott Berlin ◽  
Sam J. Bhathena ◽  
Joseph T. Judd ◽  
Beverly A. Clevidence ◽  
Renee C. Peters
Keyword(s):  
Fatty Acids ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Trans Fatty Acids ◽  
Insulin Binding ◽  
Human Erythrocyte Membrane ◽  
Erythrocyte Membrane Fluidity

Alterations of human erythrocyte membrane fluidity by oxygen-derived free radicals and calcium

1990 ◽  
Vol 8 (6) ◽  
pp. 507-514 ◽  
Author(s):  
Hiroshi Watanabe ◽  
Akira Kobayashi ◽  
Takahashi Yamamoto ◽  
Shingo Suzuki ◽  
Hideharu Hayashi ◽  
...  
Keyword(s):  
Free Radicals ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Human Erythrocyte Membrane ◽  
Oxygen Derived Free Radicals ◽  
Erythrocyte Membrane Fluidity

Human Erythrocyte Membrane Fluidity and Calcium Pump Activity in Primary Combined Hyperlipidaemia

1995 ◽  
Vol 88 (3) ◽  
pp. 307-310 ◽  
Author(s):  
S. I. Muzulu ◽  
R. F. Bing ◽  
R. I. Norman
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Serum Cholesterol ◽  
Human Erythrocyte ◽  
Calcium Pump ◽  
Membrane Cholesterol ◽  
Human Erythrocyte Membrane ◽  
Cholesterol Levels ◽  
Pump Activity ◽  
Erythrocyte Membrane Fluidity

1. Human erythrocyte membrane cholesterol, fluidity and basal and calmodulin-stimulated calcium pump (Ca2+-Mg2+-ATPase) activities were compared in 24 patients with primary combined hyperlipidaemia and 20 age-matched normolipidaemic control subjects. 2. There was no correlation between serum and membrane cholesterol. Despite the differences in serum cholesterol levels between the two groups, membrane cholesterol levels were similar. 3. 1,6-Diphenyl-1,3,5-hexatriene anisotropy was lower in the hyperlipidaemic group, suggesting increased fluidity in the hydrocarbon core of the phospholipid membrane bilayer. 4. Basal calcium pump activity was lower in the hyperlipidaemic group with increased membrane fluidity. 5. These results suggest that membrane adaptive mechanisms can maintain membrane cholesterol within a narrow range, that serum triacylglycerol is more important than serum cholesterol in determining membrane fluidity and that increased membrane fluidity reduces basal calcium pump activity.

scholarly journals Vitex Agnus-Castus L. Essential Oil Increases Human Erythrocyte Membrane Fluidity

2012 ◽  
Vol 31 (3) ◽  
pp. 222-227 ◽  
Author(s):  
Vladimir Ajdžanović ◽  
Ivan Spasojević ◽  
Jasmina Pantelić ◽  
Branka Šošić-Jurjević ◽  
Branko Filipović ◽  
...  
Keyword(s):  
Essential Oil ◽  
Stearic Acid ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Human Erythrocyte Membrane ◽  
Hydrophobic Membrane ◽  
Increased Risk ◽  
Agnus Castus ◽  
Erythrocyte Membrane Fluidity

Vitex Agnus-CastusL. Essential Oil Increases Human Erythrocyte Membrane FluidityErythrocyte membrane fluidity is related to their rheologic behavior, the dynamic quality of erythrocytes, which is tempted in hypertension and atherosclerosis. An increased risk of these and other cardiovascular diseases occurs in ageing women. Menopause-related conditions are often treated with hormone replacement therapy that may increase the risk of malignancies.Vitex agnus-castusL. essential oil contains various organic compounds (monoterpenes, sesquiterpenes and terpenoids), and is increasingly used as an alternative therapy for menopausal symptoms. These components of the oil may be incorporated into cell membranes, thereby changing the membrane fluidity. The aim of this study was to determine the effects ofVitex agnus-castusessential oil on human erythrocyte membrane fluidity at graded depths. We used Electron Paramagnetic Resonance spectroscopy and fatty acid spin probes (5-doxyl stearic acid and 12-doxyl stearic acid), whose spectra depend on membrane fluidity. After treatment withVitex agnus-castusessential oil the erythrocytes had a significant (p=0.029) and reversible increase in membrane fluidity in the deeper hydrophobic membrane regions, with no change (p>0.05) in fluidity near the membrane's hydrophilic surface. These results document increased fluidity of the human erythrocyte membrane byVitex agnus-castusessential oil, and this action may be useful in patients with menopause-related hypertension and other cardiovascular conditions.

scholarly journals Changes in Human Erythrocyte Membrane Exposed to Aqueous and Ethanolic Extracts from Uncaria tomentosa

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3189
Author(s):  
Piotr Duchnowicz ◽  
Radosław Pilarski ◽  
Jaromir Michałowicz ◽  
Bożena Bukowska
Keyword(s):  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Ethanolic Extract ◽  
Blood Purification ◽  
Human Erythrocyte Membrane ◽  
Uncaria Tomentosa ◽  
Erythrocyte Shape ◽  
Ethanolic Extracts ◽  
Erythrocyte Membrane Fluidity ◽  
Erythrocyte Plasma Membrane

Uncaria tomentosa (Willd.) DC is a woody climber species originating from South and Central America that has been used in the therapy of asthma, rheumatism, hypertension, and blood purification. Our previous study showed that U. tomentosa extracts altered human erythrocyte shape, which could be due to incorporation of the compounds contained in extracts into the erythrocyte membrane. The aim of the present study was to determine how the compounds contained in U. tomentosa extracts incorporate into the human erythrocyte membrane. The study has assessed the effect of aqueous and ethanolic extracts from leaves and bark of U. tomentosa on the osmotic resistance of the human erythrocyte, the viscosity of erythrocyte interior, and the fluidity of erythrocyte plasma membrane. Human erythrocytes were incubated with the studied extracts in the concentrations of 100, 250, and 500 µg/mL for 2, 5, and 24 h. All extracts tested caused a decrease in erythrocyte membrane fluidity and increased erythrocyte osmotic sensitivity. The ethanolic extracts from the bark and leaves increased viscosity of the erythrocytes. The largest changes in the studied parameters were observed in the cells incubated with bark ethanolic extract. We consider that the compounds from U. tomentosa extracts mainly build into the outer, hydrophilic monolayer of the erythrocyte membrane, thus protecting the erythrocytes against the adverse effects of oxidative stress.

scholarly journals Lipid Profile, Lipoprotein Subfractions, and Fluidity of Membranes in Children and Adolescents with Depressive Disorder: Effect of Omega-3 Fatty Acids in A Double-blind Randomized Controlled Study

2020 ◽  
Author(s):  
Barbora Katrenčíková ◽  
Magdaléna Vaváková ◽  
Iveta Waczulíková ◽  
Stanislav Oravec ◽  
Iveta Garaiova ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Depressive Disorder ◽  
Lipid Profile ◽  
Children And Adolescents ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Hdl Subfractions ◽  
Erythrocyte Membrane Fluidity

Depressive disorder (DD) is a psychiatric disorder whose molecular basis is not fully understood. It is assumed that reduced consumption of fish and omega-3 fatty acids (FA) is associated with DD. Other lipids like total cholesterol (TCH), LDL- and HDL-cholesterols (LDL-CH, HDL-CH) also play a role in depression. This study aimed to investigate the relationship between depressive disorder symptoms and lipid profile, LDL- and HDL-cholesterol subfractions, Paraoxonase 1 (PON1) activities and erythrocyte membrane fluidity in 58 depressive children and adolescents, as well as the effect of omega-3 FA on the monitored parameters. Depressive symptoms were assessed by the Children's Depression Inventory (CDI), lipid profile by standard biochemical procedures, LDL- and HDL-subfractions by the Lipoprint system. Basic biochemical parameters including lipid profile were compared with levels in 20 healthy children and were in the physiological range. We are the first to report that omega-3 FAs increase after 12 weeks of supplementation large HDL subfractions (anti-atherogenic) and decrease small HDL subfractions (pro-atherogenic) in depressed children. We found a negative correlation between CDI score and HDL-CH and large HDL subfraction, but not LDL-CH subfractions. CDI score was not associated with erythrocyte membrane fluidity. Our results suggest that HDL-CH and its subfractions, but not LDL-CH may play a role in the pathophysiology of depressive disorder.

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