A General Strategy for the Synthesis of Eosin Fluorescein Energy Transfer Substrates for High Sensitivity Screening of Protease Inhibitors

1994 ◽  
pp. 493-500 ◽  
Author(s):  
L.G. Contillo ◽  
D.H. Singleton ◽  
G.C. Andrews ◽  
R.W. Spencer ◽  
W.S. Faraci ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Protease Inhibitors ◽  
High Sensitivity ◽  
General Strategy

Visible-Light Photosensitized Aryl and Alkyl Decarboxylative Carbon-Heteroatom and Carbon-Carbon Bond Formations

2019 ◽  
Author(s):  
Tuhin Patra ◽  
Satobhisha Mukherjee ◽  
Jiajia Ma ◽  
Felix Strieth-Kalthoff ◽  
Frank Glorius
Keyword(s):  
Energy Transfer ◽  
Visible Light ◽  
Alkyl Radical ◽  
General Strategy ◽  
Alkyl Radicals ◽  
Bond Forming ◽  
Radical Trapping ◽  
Carbon Carbon Bond ◽  
Radical Generation ◽  
Trapping Agent

<sub>A general strategy to access both aryl and alkyl radicals by photosensitized decarboxylation of the corresponding carboxylic acids esters has been developed. An energy transfer mediated homolysis of unsymmetrical sigma-bonds for a concerted fragmentation/decarboxylation process is involved. As a result, an independent aryl/alkyl radical generation step enables a series of key C-X and C-C bond forming reactions by simply changing the radical trapping agent.</sub>

scholarly journals Plasmon-Emitter Hybrid Nanostructures of Gold Nanorod-Quantum Dots with Regulated Energy Transfer as a Universal Nano-Sensor for One-step Biomarker Detection

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 444
Author(s):  
Xuemeng Li ◽  
Yingshuting Wang ◽  
Quanying Fu ◽  
Yangyang Wang ◽  
Dongxu Ma ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Troponin I ◽  
Limit Of Detection ◽  
Refractive Index Change ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Hybrid Nanostructures ◽  
Gold Nanorod ◽  
Hybrid Nanostructure

Recently, biosensing based on weak coupling in plasmon-emitter hybrid nanostructures exhibits the merits of simplicity and high sensitivity, and attracts increasing attention as an emerging nano-sensor. In this study, we propose an innovative plasmon-regulated fluorescence resonance energy transfer (plasmon-regulated FRET) sensing strategy based on a plasmon-emitter hybrid nanostructure of gold nanorod-quantum dots (Au NR-QDs) by partially modifying QDs onto the surfaces of Au NRs. The Au NR-QDs showed good sensitivity and reversibility against refractive index change. We successfully employed the Au NR-QDs to fabricate nano-sensors for detecting a cancer biomarker of alpha fetoprotein with a limit of detection of 0.30 ng/mL, which displays that the sensitivity of the Au NR-QDs nano-sensor was effectively improved compared with the Au NRs based plasmonic sensing. Additionally, to demonstrate the universality of the plasmon-regulated FRET sensing strategy, another plasmon-emitter hybrid nano-sensor of Au nano-prism-quantum dots (Au NP-QDs) were constructed and applied for detecting a myocardial infarction biomarker of cardiac troponin I. It was first reported that the change of absorption spectra of plasmonic structure in a plasmon-emitter hybrid nanostructure was employed for analytes detection. The plasmon-regulated FRET sensing strategy described herein has potential utility to develop general sensing platforms for chemical and biological analysis.

Synthesis and Detection Experiments of a Biomolecule Detection Probe Based on Fluorescence Changes

2014 ◽  
Vol 998-999 ◽  
pp. 336-339
Author(s):  
Jun Wang ◽  
Da Hai Ren
Keyword(s):  
Energy Transfer ◽  
Fluorescence Intensity ◽  
Fluorescence Probe ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Fluorescence Probes ◽  
Biomolecule Detection ◽  
Detection Probe ◽  
Higher Sensitivity

The sensitivity of fluorescence probes built upon the resonance energy transfer is not high enough at present. We built a fluorescence probe with high sensitivity (SA-488-sub-nanogold) by means of the fluorochrome Alexa488 (SA-488) labeled by streptavidin, nanogold, and biotin-subpeptide. When the fluorescence molecule SA-488 binds with the nanogold by biotin-subpeptide, the fluorescence intensity will be suppressed because of resonance energy transfer. If there are molecules under test, the energy transfer will be blocked, by which we can get the molecule content from the fluorescence intensity. Using this probe, we acquired a lower detection limit and a higher sensitivity for biotin detection.

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