scholarly journals Double-headed protease inhibitors from black-eyed peas. VI. Singlet-singlet energy transfer and other optical studies on the structure of trypsin and chymotrypsin complexes.

1976 ◽  
Vol 251 (3) ◽  
pp. 769-775
Author(s):  
L S Gennis ◽  
C R Cantor
Author(s):  
L.G. Contillo ◽  
D.H. Singleton ◽  
G.C. Andrews ◽  
R.W. Spencer ◽  
W.S. Faraci ◽  
...  

2019 ◽  
Vol 9 (2) ◽  
pp. 80-93 ◽  
Author(s):  
E.K. Girija ◽  
S. Karthi ◽  
D. Karthickraja ◽  
G.A. Kumar ◽  
D.K. Sardar ◽  
...  

Aim: To synthesize biocompatible nanoparticles of FAp co-doped with Yb/Er and Nd/Yb for bioimaging applications. Methods: Yb/Er FAp and Nd/Yb FAp was synthesized using microwave assisted wet precipitation and hydrothermal method respectively. Trisodium citrate was used as an organic modifier for the synthesis and then subjected to heat treatment for optical activation. For optical studies, Yb/Er FAp system was excited at 980 nm and Nd/Yb FAp at 800 nm. Results: In the case of Nd/Yb FAp the host matrix absorption and emission was observed, hence Nd/Yb was synthesized without citrate. On heat treatment of this for optical activation studies, when the Yb3+ concentration was increased to 10 mol%, the YbPO4 secondary phase was found to appear. Although, the Yb/Er FAp system resulted in large grain growth, no such grain growth was observed in Nd/Yb FAp and the grains were within the nano size regime even after heat treatment. Conclusion: Both the systems showed successful energy transfer from sensitizer to activator with a quantum yield of 74% for Yb/Er FAp and energy transfer efficiency of 71% for Nd/Yb FAp system. Both the samples were found to be cytocompatible and has the potential for using as probes for bioimaging applications.


2011 ◽  
Vol 21 (4) ◽  
pp. 1162-1170 ◽  
Author(s):  
José Planelles-Aragó ◽  
Eloisa Cordoncillo ◽  
Rute A. S. Ferreira ◽  
Luís D. Carlos ◽  
Purificación Escribano

Author(s):  
R.D. Leapman ◽  
P. Rez ◽  
D.F. Mayers

Microanalysis by EELS has been developing rapidly and though the general form of the spectrum is now understood there is a need to put the technique on a more quantitative basis (1,2). Certain aspects important for microanalysis include: (i) accurate determination of the partial cross sections, σx(α,ΔE) for core excitation when scattering lies inside collection angle a and energy range ΔE above the edge, (ii) behavior of the background intensity due to excitation of less strongly bound electrons, necessary for extrapolation beneath the signal of interest, (iii) departures from the simple hydrogenic K-edge seen in L and M losses, effecting σx and complicating microanalysis. Such problems might be approached empirically but here we describe how computation can elucidate the spectrum shape.The inelastic cross section differential with respect to energy transfer E and momentum transfer q for electrons of energy E0 and velocity v can be written as


Author(s):  
S.W. French ◽  
N.C. Benson ◽  
C. Davis-Scibienski

Previous SEM studies of liver cytoskeletal elements have encountered technical difficulties such as variable metal coating and heat damage which occurs during metal deposition. The majority of studies involving evaluation of the cell cytoskeleton have been limited to cells which could be isolated, maintained in culture as a monolayer and thus easily extracted. Detergent extraction of excised tissue by immersion has often been unsatisfactory beyond the depth of several cells. These disadvantages have been avoided in the present study. Whole C3H mouse livers were perfused in situ with 0.5% Triton X-100 in a modified Jahn's buffer including protease inhibitors. Perfusion was continued for 1 to 2 hours at ambient temperature. The liver was then perfused with a 2% buffered gluteraldehyde solution. Liver samples including spontaneous tumors were then maintained in buffered gluteraldehyde for 2 hours. Samples were processed for SEM and TEM using the modified thicarbohydrazide procedure of Malich and Wilson, cryofractured, and critical point dried (CPD). Some samples were mechanically fractured after CPD.


Physica ◽  
1954 ◽  
Vol 3 (7-12) ◽  
pp. 1169-1180
Author(s):  
F DEWETTE ◽  
Z SLAWSKY

Sign in / Sign up

Export Citation Format

Share Document