Expression of vitellogenin and cortical rod proteins during induced ovarian development by eyestalk ablation in the kuruma prawn, Marsupenaeus japonicus

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

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Effects of bilateral eyestalk ablation on molting and ovarian development in the giant freshwater prawn, Macrobrachium rosenbergii

Fisheries Science ◽

10.1046/j.1444-2906.2001.00370.x ◽

2001 ◽

Vol 67 (6) ◽

pp. 1125-1135 ◽

Cited By ~ 34

Author(s):

takuji Okumura ◽

katsumi Aida

Keyword(s):

Ovarian Development ◽

Macrobrachium Rosenbergii ◽

Freshwater Prawn ◽

Eyestalk Ablation ◽

Giant Freshwater Prawn

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RANGSANGAN PERKEMBANGAN OVARI UDANG PUTIH, Litopenaeus vannamei DENGAN PENYUNTIKAN ESTRADIOL-17β

Jurnal Riset Akuakultur ◽

10.15578/jra.2.3.2007.349-358 ◽

2016 ◽

Vol 2 (3) ◽

pp. 349

Author(s):

Tarsim Tarsim ◽

Muhammad Zairin Junior ◽

Etty Riani

Keyword(s):

Body Weight ◽

Litopenaeus Vannamei ◽

Ovarian Development ◽

Developmental Stages ◽

Mature Oocyte ◽

White Shrimp ◽

Early Developmental Stage ◽

Eyestalk Ablation ◽

Maturation Index ◽

Estradiol 17Β

Studi ini bertujuan untuk mengetahui pengaruh penyuntikan hormon estradiol-17β terhadap perkembangan ovari udang putih (Litopenaeus vannamei). Dosis estradiol17β yang digunakan adalah 0,05 μg/g; 0,10 μg/g; 0,25 μg/g bobot tubuh dan kontrol Hasil penelitian menunjukkan bahwa penyuntikan estradiol-17β berpengaruh terhadap perkembangan gonad. Indeks maturasi pada perlakuan dosis 0,10 μg/g and 0,25 μg/ g bobot tubuh lebih besar (75,00% dan 66,67%) dibanding kontrol. Peningkatan diameter oosit terlihat nyata pada TKG I dan II. Meskipun pada TKG III dan IV peningkatan oosit tidak terlihat nyata, tetapi proporsi oosit matang lebih besar. Hal ini menunjukkan bahwa pemberian estradiol-17β pada induk udang ablasi menghasilkan telur yang lebih cepat berkembang dibandingkan jika hanya menggunakan ablasi. Penyuntikan estradiol-17β sangat berpengaruh pada awal perkembangan gonad. Hal ini menunjukkan bahwa estradiol-17β berperan penting dalam merangsang endogenous vitelogenesis.The present study analyzed the effect of estradiol-17 β injection on ovarian development of white shrimp, Litopenaeus vannamei. Estradiol-17 β dose of 0.05 υ g/g, 0.10 μ g/g, 0.25 μ g/g body weight and the control was used, with 15 females broodstock of each. The result showed that ovarian development affected by estradiol-17 β injection. Maturation index in dose of 0.10 μ g/g and 0.25 μ g/g body weight was 75.00% and 66.67% respectively and higher than that of control. Oocytes diameter increased significantly on stage I and stage II, although oocytes diameter in stage III and IV was no significant different but the proportion of mature oocyte higher than that of control. It suggested that estradiol-17 β gave much more developed conditions in oocytes developmental stages and size, compared to control with unilateral eyestalk ablation only. The dominant effect is in early developmental stage of oocyte. It indicates that estradiol-17 β is important to induction of endogenous vitellogenesis in white shrimp.

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Polychaete consumption increased prostaglandin biosynthesis in female Penaeus monodon

Reproduction ◽

10.1530/rep-20-0217 ◽

2020 ◽

Vol 160 (6) ◽

pp. 873-885

Author(s):

Pacharawan Deenarn ◽

Punsa Tobwor ◽

Vanicha Vichai ◽

Suwanchai Phomklad ◽

Panomkorn Chaitongsakul ◽

...

Keyword(s):

Fatty Acid ◽

Ovarian Development ◽

Penaeus Monodon ◽

Prostaglandin E ◽

Prostaglandin Biosynthesis ◽

Eyestalk Ablation ◽

Regulatory Pathways ◽

Feed Pellets ◽

Prostaglandin F ◽

The Impact

The polychaete Perinereis nuntia is preferred over commercial feed pellets for boosting ovarian maturation of the female black tiger shrimp Penaeus monodon. High levels of prostaglandins in polychaetes are believed to enhance shrimp ovarian development. However, the impact of polychaete feeding on shrimp prostaglandin biosynthesis and fatty acid regulatory pathways have yet to be investigated. As polychaetes contain higher levels of arachidonic acid (ARA), eicosapentaenoic acid (EPA), prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) than feed pellets, we examined the effects of polychaete feeding alone and in combination with eyestalk ablation on shrimp hepatopancreases and ovaries. Shrimp fed with polychaetes contained higher levels of EPA, PGE2 and PGF2α in hepatopancreases than those of pellet-fed shrimp. Similarly, higher levels of ARA and higher transcription levels of cyclooxygenase (COX) and prostaglandin F synthase (PGFS) were detected in ovaries of polychaete-fed shrimp compared to those of pellet-fed shrimp. The combination of polychaete-feeding and eyestalk ablation, commonly practiced to induce ovarian development, increased levels of ARA and EPA and transcription levels of COX in hepatopancreases and ovaries of polychaete-fed shrimp compared to those of pellet-fed shrimp. In ovaries, prostaglandin biosynthesis gene transcripts were induced by polychaete feeding while transcriptional levels of fatty acid regulatory genes were regulated by shrimp feed and eyestalk ablation. Our findings not only elucidate the effects of polychaete consumption on shrimp prostaglandin biosynthesis and fatty acid regulatory pathways during larvae production, but also suggests that high levels of dietary ARA, EPA and prostaglandins are essential during P. monodon ovarian development.

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