Effect of bilateral eyestalk ablation on ovarian development and moulting in early and late intermoult stages of female spiny lobster Panulirus homarus (Linnaeus, 1758)

2016 ◽  
Vol 60 (3) ◽  
pp. 238-242 ◽  
Author(s):  
Rachel Fernandez ◽  
E. V. Radhakrishnan
Keyword(s):  
Ovarian Development ◽  
Spiny Lobster ◽  
Eyestalk Ablation

Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Reproduction ◽  
2018 ◽  
Author(s):  
Pacharawan Deenarn ◽  
Punsa Tobwor ◽  
Rungnapa Leelatanawit ◽  
Somjai Wongtriphop ◽  
Jutatip Khudet ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ovarian Development ◽  
Penaeus Monodon ◽  
Chain Fatty Acid ◽  
Regulatory Genes ◽  
Ovarian Maturation ◽  
Long Chain Fatty Acid ◽  
Eyestalk Ablation ◽  
Regulatory Pathways ◽  
Expression Levels

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

scholarly journals PEMIJAHAN INDUK, PROFIL KOLESTEROL, DAN ASAM LEMAK TELUR LOBSTER PASIR (Panulirus homarus) HASIL BUDIDAYA

2018 ◽  
Vol 13 (3) ◽  
pp. 219
Author(s):  
Yudha Trinoegraha Adiputra ◽  
Muhammad Zairin Jr. ◽  
Muhammad Agus Suprayudi ◽  
Wasmen Manalu ◽  
Widanarni Widanarni
Keyword(s):  
Fatty Acids ◽  
Full Moon ◽  
Spiny Lobster ◽  
Eyestalk Ablation ◽  
Early Indicator ◽  
Mass Spawning ◽  
New Moon ◽  
Low Incidence

Pembenihan lobster pasir (Panulirus homarus) belum berkembang di Indonesia karena terbatasnya teknik pemijahan induk dan belum diketahuinya tingkat keberhasilan induk betina membawa telur dan profil telur hasil pemeliharaan. Penelitian bertujuan untuk mempelajari pengaruh ablasi satu tangkai mata, metode pemijahan massal dan individual pada bulan gelap dan terang pada induk berganti kulit dan membawa telur, serta konsentrasi kolesterol dan asam-asam lemak telur pada lama pengeraman berbeda. Induk betina dan jantan dipakai secara terus-menerus saat percobaan pemijahan. Pemijahan pertama menggunakan pemijahan dengan rasio 2:1 pada bulan gelap. Percobaan kedua dan ketiga menggunakan pemijahan massal selama bulan terang dan gelap. Profil telur dibandingkan konsentrasi kolesterol dan asam lemaknya pada 1, 2, dan 3-4 minggu pengeraman. Hasil penelitian menunjukkan ablasi tangkai mata dapat menstimulasi pemijahan individual dan massal yang didahului dengan ganti kulit pada induk betina sebagai indikator pemijahan. Pemijahan massal pada bulan gelap menghasilkan induk betina membawa telur terbanyak dibandingkan pemijahan individual pada bulan gelap dan pemijahan massal pada bulan terang. Konsentrasi kolesterol dan asam-asam lemak telur mengalami perubahan mengikuti lama pengeraman oleh induk yang menunjukkan terjadinya peningkatan kematangan telur (P<0,05).Spiny lobster (Panulirus homarus) hatchery has not been developed in Indonesia. Major constraints were limited broodstock spawning techniques, low incidence of eggs-berried broodstock, and unknown eggs profile. This study aimed to evaluate the effects of eyestalk ablation, spawning methods and moon circulation to molted, the number of eggs berried of females, and eggs-berried cholesterol and fatty acids profiles. Individual and mass spawning during full and new moon were used in this studies. Female and male broodstocks were used for consecutive spawning with ratio 2:1. The first study was used individual spawning during new moon. The second and third studies were used mass spawning during full moon and new moon, respectively. Eggs-berried profile compared concentration of cholesterol and fatty acids during 1, 2, and 3-4 weeks. Results showed eyestalk ablation stimulated individual or mass spawned and molted of female as early indicator of spawning. Full moon and mass spawning supported more eggs-berried female broodstock than that of other methods. Cholesterol and fatty acids showed different concentration within 1, 2, and 3-4 weeks of eggs-berried that supported eggs maturity (P<0.05).

Effects of photoperiod and temperature on ovarian development and spawning of the Japanese spiny lobster Panulirus japonicus

Aquaculture ◽  
2002 ◽  
Vol 205 (3-4) ◽  
pp. 385-398 ◽  
Author(s):  
Hirokazu Matsuda ◽  
Taisuke Takenouchi ◽  
Takashi Yamakawa
Keyword(s):  
Ovarian Development ◽  
Spiny Lobster ◽  
Panulirus Japonicus

scholarly journals RANGSANGAN PERKEMBANGAN OVARI UDANG PUTIH, Litopenaeus vannamei DENGAN PENYUNTIKAN ESTRADIOL-17β

2016 ◽  
Vol 2 (3) ◽  
pp. 349
Author(s):  
Tarsim Tarsim ◽  
Muhammad Zairin Junior ◽  
Etty Riani
Keyword(s):  
Body Weight ◽  
Litopenaeus Vannamei ◽  
Ovarian Development ◽  
Developmental Stages ◽  
Mature Oocyte ◽  
White Shrimp ◽  
Early Developmental Stage ◽  
Eyestalk Ablation ◽  
Maturation Index ◽  
Estradiol 17Β

Studi ini bertujuan untuk mengetahui pengaruh penyuntikan hormon estradiol-17β terhadap perkembangan ovari udang putih (Litopenaeus vannamei). Dosis estradiol17β yang digunakan adalah 0,05 μg/g; 0,10 μg/g; 0,25 μg/g bobot tubuh dan kontrol Hasil penelitian menunjukkan bahwa penyuntikan estradiol-17β berpengaruh terhadap perkembangan gonad. Indeks maturasi pada perlakuan dosis 0,10 μg/g and 0,25 μg/ g bobot tubuh lebih besar (75,00% dan 66,67%) dibanding kontrol. Peningkatan diameter oosit terlihat nyata pada TKG I dan II. Meskipun pada TKG III dan IV peningkatan oosit tidak terlihat nyata, tetapi proporsi oosit matang lebih besar. Hal ini menunjukkan bahwa pemberian estradiol-17β pada induk udang ablasi menghasilkan telur yang lebih cepat berkembang dibandingkan jika hanya menggunakan ablasi. Penyuntikan estradiol-17β sangat berpengaruh pada awal perkembangan gonad. Hal ini menunjukkan bahwa estradiol-17β berperan penting dalam merangsang endogenous vitelogenesis.The present study analyzed the effect of estradiol-17 β injection on ovarian development of white shrimp, Litopenaeus vannamei. Estradiol-17 β dose of 0.05 υ g/g, 0.10 μ g/g, 0.25 μ g/g body weight and the control was used, with 15 females broodstock of each. The result showed that ovarian development affected by estradiol-17 β injection. Maturation index in dose of 0.10 μ g/g and 0.25 μ g/g body weight was 75.00% and 66.67% respectively and higher than that of control. Oocytes diameter increased significantly on stage I and stage II, although oocytes diameter in stage III and IV was no significant different but the proportion of mature oocyte higher than that of control. It suggested that estradiol-17 β gave much more developed conditions in oocytes developmental stages and size, compared to control with unilateral eyestalk ablation only. The dominant effect is in early developmental stage of oocyte. It indicates that estradiol-17 β is important to induction of endogenous vitellogenesis in white shrimp.

Oogenesis and ovarian development cycle of the spiny lobster Panulirus japonicus (Decapoda:Palinuridae)

1997 ◽  
Vol 48 (8) ◽  
pp. 875 ◽  
Author(s):  
Megumi Minagawa ◽  
Motohiko Sano
Keyword(s):  
Ovarian Development ◽  
Gonadosomatic Index ◽  
Spiny Lobster ◽  
Light And Electron Microscopy ◽  
Morphological Characteristics ◽  
Dense Granules ◽  
Development Cycle ◽  
Maturation Stages ◽  
Panulirus Japonicus ◽  
Yolk Platelet

Oogenesis and ovarian development in female Panulirus japonicus were examined by light and electron microscopy. Eight substages (oogonium, bouquet, chromatin nucleolus, oil globule, pre-yolk platelet, yolk platelet, pre-maturation and maturation) were distinguished in the typical process of oogenesis (multiplication, pre-vitellogenesis, vitellogenesis and maturation stages). Yolk accumulation started at the late pre-yolk platelet substage, when electron-dense granules appeared. Yolk granules seemed to be accumulated in two ways, being produced endogenously by the abundant rough endoplasmic reticulum during vitellogenesis and exogenously by micropinocytosis from the yolk platelet to pre-maturation substages. Ovulation occurred after oocytes became mature (i.e. after the metaphase of the primary maturation division was reached). The diameter of mature oocytes was 465–477 µm. The seasonal ovarian development cycle was divided into seven stages: inactive, developing, ripe, re- developing, re-ripe, spawned and recovery. The morphological characteristics relating to the gonadosomatic index (GSI) of each stage are described (GSI was calculated by the formula I = W × 105/L3, where I represents GSI, W is the gonad weight in grams, and L is the carapace length in millimetres). Estimated GSI values ranged from 11.3 to 12.4 in individuals with mature oocytes, and the 99% confidence intervals for GSI values of adjacent oogenesis substages did not overlap. GSI values at the developing and ripe stages were significantly larger than those at the re-developing and re-ripe stages, respectively.

scholarly journals Polychaete consumption increased prostaglandin biosynthesis in female Penaeus monodon

Reproduction ◽  
2020 ◽  
Vol 160 (6) ◽  
pp. 873-885
Author(s):  
Pacharawan Deenarn ◽  
Punsa Tobwor ◽  
Vanicha Vichai ◽  
Suwanchai Phomklad ◽  
Panomkorn Chaitongsakul ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ovarian Development ◽  
Penaeus Monodon ◽  
Prostaglandin E ◽  
Prostaglandin Biosynthesis ◽  
Eyestalk Ablation ◽  
Regulatory Pathways ◽  
Feed Pellets ◽  
Prostaglandin F ◽  
The Impact

The polychaete Perinereis nuntia is preferred over commercial feed pellets for boosting ovarian maturation of the female black tiger shrimp Penaeus monodon. High levels of prostaglandins in polychaetes are believed to enhance shrimp ovarian development. However, the impact of polychaete feeding on shrimp prostaglandin biosynthesis and fatty acid regulatory pathways have yet to be investigated. As polychaetes contain higher levels of arachidonic acid (ARA), eicosapentaenoic acid (EPA), prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) than feed pellets, we examined the effects of polychaete feeding alone and in combination with eyestalk ablation on shrimp hepatopancreases and ovaries. Shrimp fed with polychaetes contained higher levels of EPA, PGE2 and PGF2α in hepatopancreases than those of pellet-fed shrimp. Similarly, higher levels of ARA and higher transcription levels of cyclooxygenase (COX) and prostaglandin F synthase (PGFS) were detected in ovaries of polychaete-fed shrimp compared to those of pellet-fed shrimp. The combination of polychaete-feeding and eyestalk ablation, commonly practiced to induce ovarian development, increased levels of ARA and EPA and transcription levels of COX in hepatopancreases and ovaries of polychaete-fed shrimp compared to those of pellet-fed shrimp. In ovaries, prostaglandin biosynthesis gene transcripts were induced by polychaete feeding while transcriptional levels of fatty acid regulatory genes were regulated by shrimp feed and eyestalk ablation. Our findings not only elucidate the effects of polychaete consumption on shrimp prostaglandin biosynthesis and fatty acid regulatory pathways during larvae production, but also suggests that high levels of dietary ARA, EPA and prostaglandins are essential during P. monodon ovarian development.

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