Genetically magnetic control of neural system via TRPV4 activation with magnetic nanoparticles

Ferrite magnetic nanoparticles (Fe3O4or iron (II,III) oxide; 15–25 nm of diameter) were developed. These magnetic nanoparticles are a potential vehicle for magnetically induced target aggregation in living animals. In this preliminary study, the radiochemical purity for the radiolabeled magnetic nanoparticles was examined, and the possibility of the magnetically induced targeting of the radio-nanoparticles was evaluated. Our results showed that radiolabeled ferrite nanoparticles can be used as magnetic targeting agents with high labeling efficiency and stability. These particles can be distributed within living animals via intravenous injection, and the biodistribution of the particles can be potentially controlled by external magnetism. These evaluations will be the groundwork for the future development of delivery techniques for radiopharmaceuticals through external magnetic control.

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Using Antibiotic Conjugated Magnetic Nanoparticles and a Magnetic Field for the Treatment of Bone Prosthetic Infections

MRS Proceedings ◽

10.1557/opl.2011.521 ◽

2011 ◽

Vol 1316 ◽

Author(s):

Erik N. Taylor ◽

George E. Aninwene ◽

Thomas J. Webster

Keyword(s):

Magnetic Field ◽

Magnetic Nanoparticles ◽

Medical Devices ◽

Superparamagnetic Iron Oxide ◽

Microbial Colonization ◽

Medical Community ◽

Oxide Nanoparticles ◽

Magnetic Control ◽

New Type ◽

Multifunctional Properties

ABSTRACTBacterial infection of bone (called osteomyelitis) is of great concern to the medical community. In addition to bone, numerous medical devices are susceptible to microbial colonization when implanted. These infections are chronic since bacteria form a robust adhesion to surfaces, can be protected by sticky slime matrix (called a biofilm) from the body’s immune system (which would otherwise naturally clear the bacteria), and antibiotic treatments may not resolve such infections (due to antibiotic resistance). Here, the multifunctional properties of magnetic nanoparticles (termed here superparamagnetic iron oxide nanoparticles, or SPION) will be explored for their antibacterial activity, magnetic properties, and drug deliverable properties. This study provides a first step towards the development of a new type of pharmaceutical useful for orthopedic or other device related infections by demonstrating physical (magnetic) control of antibiotics towards bacteria and biofilms.

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Magnetic control of cellular processes using biofunctional nanoparticles

Chemical Science ◽

10.1039/c7sc01462g ◽

2017 ◽

Vol 8 (11) ◽

pp. 7330-7338 ◽

Cited By ~ 34

Author(s):

Cornelia Monzel ◽

Chiara Vicario ◽

Jacob Piehler ◽

Mathieu Coppey ◽

Maxime Dahan

Keyword(s):

Remote Control ◽

Magnetic Nanoparticles ◽

Magnetic Fields ◽

Magnetic Control ◽

Cellular Functions ◽

Cellular Processes ◽

Molecular Stimulation ◽

Biofunctional Nanoparticles

Remote control of cellular functionsviamechanical, thermal or molecular stimulation is accomplished using biofunctional magnetic nanoparticles (MNPs) and magnetic fields.

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Sub-second multi-channel magnetic control of select neural circuits in behaving flies

10.21203/rs.3.rs-324432/v1 ◽

2021 ◽

Author(s):

Charles Sebesta ◽

Daniel Torres ◽

Boshuo Wang ◽

Zhongxi Li ◽

Guillaume Duret ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Neural Circuits ◽

Cell Activity ◽

Temperature Sensitive ◽

Magnetic Control ◽

Deep Tissue ◽

Minimal Invasiveness ◽

Non Invasive ◽

Freely Behaving

Abstract Precisely timed activation of genetically targeted cells is a powerful tool for studying neural circuits and controlling cell-based therapies. Magnetic control of cell activity or “magnetogenetics” using magnetic nanoparticle heating of temperature-sensitive ion channels enables remote, non-invasive activation of neurons for deep-tissue applications and studies of freely behaving animals. However, the in vivo response time of thermal magnetogenetics is currently tens of seconds, which prevents the precise temporal modulation of neural activity similar to light-based optogenetics. Moreover, magnetogenetics has not provided a means to selectively activate multiple channels to drive behavior. Here we demonstrate that by combining magnetic nanoparticles with a rate-sensitive thermoreceptor (TRPA1-A) it is possible to achieve sub-second behavioral responses in Drosophila melanogaster. Furthermore, by tuning the properties of magnetic nanoparticles to respond to different magnetic field strengths and frequencies, we can achieve fast, multi-channel stimulation, analogous to optogenetic stimulation with different wavelengths of light. These results bring magnetogenetics closer to the temporal resolution and multiplexed stimulation possible with optogenetics while maintaining the minimal invasiveness and deep-tissue stimulation only possible by magnetic control.

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Sub-second multi-channel magnetic control of select neural circuits in behaving flies

10.1101/2021.03.15.435264 ◽

2021 ◽

Author(s):

Charles Sebesta ◽

Daniel Torres ◽

Boshuo Wang ◽

Zhongxi Li ◽

Guillaume Duret ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Neural Circuits ◽

Cell Activity ◽

Temperature Sensitive ◽

Magnetic Control ◽

Deep Tissue ◽

Minimal Invasiveness ◽

Non Invasive ◽

Freely Behaving

Precisely timed activation of genetically targeted cells is a powerful tool for studying neural circuits and controlling cell-based therapies. Magnetic control of cell activity or "magnetogenetics" using magnetic nanoparticle heating of temperature-sensitive ion channels enables remote, non-invasive activation of neurons for deep-tissue applications and studies of freely behaving animals. However, the in vivo response time of thermal magnetogenetics is currently tens of seconds, which prevents the precise temporal modulation of neural activity similar to light-based optogenetics. Moreover, magnetogenetics has not provided a means to selectively activate multiple channels to drive behavior. Here we demonstrate that by combining magnetic nanoparticles with a rate-sensitive thermoreceptor (TRPA1-A) it is possible to achieve sub-second behavioral responses in Drosophila melanogaster. Furthermore, by tuning the properties of magnetic nanoparticles to respond to different magnetic field strengths and frequencies, we can achieve fast, multi-channel stimulation, analogous to optogenetic stimulation with different wavelengths of light. These results bring magnetogenetics closer to the temporal resolution and multiplexed stimulation possible with optogenetics while maintaining the minimal invasiveness and deep-tissue stimulation only possible by magnetic control.

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Predicting spatial activity patterns in a neural map from a probabilistic atlas of 3-D reconstructed neurons

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100140439 ◽

1995 ◽

Vol 53 ◽

pp. 812-813

Author(s):

G. Jacobs ◽

F. Theunissen

Keyword(s):

Activity Patterns ◽

Three Dimensional ◽

Sensory System ◽

Neural System ◽

Probabilistic Atlas ◽

Uniform Sample ◽

Dimensional Reconstruction ◽

The Time Domain ◽

And Function ◽

Visualization Techniques

In order to understand how the algorithms underlying neural computation are implemented within any neural system, it is necessary to understand details of the anatomy, physiology and global organization of the neurons from which the system is constructed. Information is represented in neural systems by patterns of activity that vary in both their spatial extent and in the time domain. One of the great challenges to microscopists is to devise methods for imaging these patterns of activity and to correlate them with the underlying neuroanatomy and physiology. We have addressed this problem by using a combination of three dimensional reconstruction techniques, quantitative analysis and computer visualization techniques to build a probabilistic atlas of a neural map in an insect sensory system. The principal goal of this study was to derive a quantitative representation of the map, based on a uniform sample of afferents that was of sufficient size to allow statistically meaningful analyses of the relationships between structure and function.

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