Acetyl-coenzyme a carboxylase activity in whole blood and its dependency on the biotin status of chickens

1975 ◽  
Vol 66 (1) ◽  
pp. 368-374 ◽  
Author(s):  
D. Glatzle ◽  
M. Frigg
Keyword(s):  
Whole Blood ◽  
Coenzyme A ◽  
Carboxylase Activity ◽  
Acetyl Coenzyme A ◽  
Acetyl Coenzyme ◽  
Acetyl Coenzyme A Carboxylase

Effect of Sethoxydim and Haloxyfop on Acetyl-Coenzyme a Carboxylase Activity inFestucaSpecies

Weed Science ◽  
1989 ◽  
Vol 37 (4) ◽  
pp. 512-516 ◽  
Author(s):  
David E. Stoltenberg ◽  
John W. Gronwald ◽  
Donald L. Wyse ◽  
James D. Burton ◽  
David A. Somers ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Tall Fescue ◽  
Coenzyme A ◽  
Carboxylase Activity ◽  
Acetyl Coenzyme A ◽  
Red Fescue ◽  
Acetyl Coenzyme ◽  
Cell Extracts ◽  
Site Of Action ◽  
Acetyl Coenzyme A Carboxylase

In greenhouse studies, the calculated I50(herbicide application resulting in 50% inhibition of shoot regrowth) in tall fescue was approximately 0.004 kg/ha for both sethoxydim and haloxyfop. In red fescue, the I50for sethoxydim and haloxyfop was 9.4 kg/ha and 0.04 kg/ha, respectively. As measured in crude cell extracts of tall fescue, incorporation of14C-acetyl-coenzyme A into fatty acids was inhibited 62 and 71% by 10 μM sethoxydim and 10 μM haloxyfop, respectively. In red fescue, 10 μM haloxyfop inhibited14C-acetyl-CoA incorporation into fatty acids by 29%, whereas 10 μM sethoxydim had no effect. The I50for inhibition of acetyl-coenzyme A carboxylase activity in tall fescue with sethoxydim and haloxyfop was 6.9 and 5.8 μM, respectively. In red fescue the I50for haloxyfop was 118 μM. Sethoxydim concentrations as high as 1 mM had little effect on acetyl-coenzyme A carboxylase activity in red fescue. These results suggest that acetyl-coenzyme A carboxylase is a sensitive site of action for sethoxydim and haloxyfop in tall fescue, and that tolerance to these herbicides in red fescue is due to the presence of a more tolerant form of the enzyme.

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