The requirement for lymphotoxin and Peyer's patches in the development of intestinal lamina propria lymphocytes

2000 ◽  
Vol 118 (4) ◽  
pp. A361
Author(s):  
Rodney David Newberry ◽  
Robin G. Lorenz
Keyword(s):  
Lamina Propria ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Intestinal Lamina Propria ◽  
Lamina Propria Lymphocytes

scholarly journals P073 Compartmentalised human gut immunity: studies of innate and adaptive lymphocyte distribution in the epithelium, lamina propria and GALT of healthy gut mucosa by flow cytometry

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S172-S172
Author(s):  
A Carrasco Garcia ◽  
A Rao ◽  
E Kokkinou ◽  
S Haapaniemi ◽  
U Lindforss ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Lamina Propria ◽  
Immune Cell ◽  
Cd8 T Cell ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Human Gut ◽  
Cell Ratio ◽  
Health And Disease

Abstract Background The human gut mucosal immune system is compartmentalised in distinct and specialised immune niches. The epithelium and the lamina propria have been proposed as effector sites, while gut-associated lymphoid tissues (GALTs) constitute inductive immune niches. The major mucosal GALTs are the Peyer’s patches in the ileum and the colonic isolated lymphoid follicles (ILFs), scattered in the submucosa of the colon. The majority of studies of human gut immune function in health and disease have analysed unfractionated mucosal tissue samples. Hence, in contrast to mice, little is known about compartmentalised immune cell specialisation in the human gut. The aim of this study was to use novel dissection methods to analyse separate human gut immune niches. Methods Macroscopically healthy margins from colorectal cancer colectomies were obtained at a minimum distance of 10 cm from the tumour border. After faeces, mucus, fat and muscle removal, Peyer’s patches were identified and dissected using a stereomicroscope (based on Keita et al., Lab Invest, 2006). Colonic mucosa and submucosa (containing ILFs) fractions were mechanically separated by forceps (based on the method developed by Fenton et al., Immunity, under revision). Isolation of epithelial and lamina propria fractions from the mucosal compartment was performed by calcium chelation (DTT and EDTA) and enzymatic digestion (Collagenase II and DNAse), respectively. Cell suspensions from each fraction were analysed by flow cytometry (BD LSR-Fortessa and BD FACSymphony). Results As expected, mucosal GALTs were characterised by an enrichment of germinal centre B cells (CD19+CD20+CD38+), lymphoid tissue-like innate lymphoid cells (Lin−CD127+CD117+Nrp1+) and a higher CD4+/CD8+ T-cell ratio vs. mucosa, whereas the mucosal fraction was enriched for plasma cells (CD19+CD20−CD38high) and distinguished by a decreased CD4+/CD8+ T-cell ratio as compared with the GALT in both ileum and colon. CD19+/CD3+ ratios were only higher in Peyer’s patches but not in colonic submucosa enriched with ILFs, possibly due to the smaller size of the B-cell follicles in the latter. The intraepithelial compartment lacked B cells and contained more γδ-T cells as compared with the GALT and lamina propria. Conclusion We have used novel dissection methods in human intestinal tissues that reveal a compartmentalised immune cell specialisation that is in line with what has previously been described in mice. The method will allow for future deeper analysis of the human gut immune niches in health and disease, such as in inflammatory bowel disease.

Effect of bombesin on [Ca++]i and proliferation of lymphocytes isolated from lamina propria, peyer's patches, and spleen

1992 ◽  
Vol 40 (2) ◽  
pp. 273
Author(s):  
R Waetke ◽  
RM Liehr ◽  
K Bordasch ◽  
H Haller ◽  
M Zeitz ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Proliferation Of Lymphocytes

Effects of somatostatin on human intestinal lamina propria lymphocytes. Modulation of lymphocyte activation

1991 ◽  
Vol 31 (3) ◽  
pp. 211-219 ◽  
Author(s):  
S. Fais ◽  
B. Annibale ◽  
M. Boirivant ◽  
A. Santoro ◽  
F. Pallone ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Lymphocyte Activation ◽  
Intestinal Lamina Propria ◽  
Lamina Propria Lymphocytes

scholarly journals Peyer's patch eosinophils: identification, characterization, and regulation by mucosal allergen exposure, interleukin-5, and eotaxin

Blood ◽  
2000 ◽  
Vol 96 (4) ◽  
pp. 1538-1544
Author(s):  
Anil Mishra ◽  
Simon P. Hogan ◽  
Eric B. Brandt ◽  
Marc E. Rothenberg
Keyword(s):  
Gastrointestinal Tract ◽  
Lamina Propria ◽  
Allergen Exposure ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Outer Cortex ◽  
Interleukin 5 ◽  
Marked Accumulation ◽  
Low Levels

The gastrointestinal immune system is traditionally thought to be composed of lymphocytes located within Peyer's patches and the lamina propria. We have recently reported that eosinophils also reside in the gastrointestinal tract during healthy states, in particular, within the lamina propria, and that these cells substantially increase after oral allergen exposure. We now demonstrate the presence of eosinophils in Peyer's patches and characterize the signals that regulate the accumulation of eosinophils in Peyer's patches. In contrast to the lamina propria, intestinal Peyer's patches have very low levels of eosinophils under healthy states. However, elevated levels of interleukin-5 (IL-5), generated by transgenic or pharmacologic approaches, result in a dramatic increase in eosinophil levels in Peyer's patches. Most eosinophils are located in the outer cortex and interfollicular regions of the Peyer's patches. To dissect the mechanism of eosinophil trafficking to Peyer's patches, the role of eotaxin was examined. Mice transgenic for IL-5 and genetically deficient in eotaxin were found to have reduced levels of eosinophils in Peyer's patches compared with IL-5-transgenic mice. To prove that eosinophils also traffic to Peyer's patches in wild-type mice, allergic hypersensitivity was induced and Peyer's patches were examined. Exposure to mucosal allergen promoted marked accumulation of eosinophils in Peyer's patches and this process was attenuated in eotaxin-deficient mice. In summary, these data demonstrate that elevated levels of IL-5 and mucosal allergen exposure promote eotaxin-dependent eosinophil trafficking to Peyer's patches. These studies suggest that eosinophils may cooperate with lymphocytes in the development of mucosal immune responses in the gastrointestinal tract.

scholarly journals Role of acetylcholine on the isotype switching in Peyer's patches and lamina propria for IgA secretion in small intestine of BALB/c mice.

2015 ◽  
Vol 6 ◽  
Author(s):  
Salas Pimentel Marisol ◽  
Reséndiz Albor Aldo ◽  
Arciniega Martínez Ivonne ◽  
Martínez Becerril Elia ◽  
García Fonseca Alan ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lamina Propria ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Isotype Switching

scholarly journals Peyer's patch eosinophils: identification, characterization, and regulation by mucosal allergen exposure, interleukin-5, and eotaxin

Blood ◽  
2000 ◽  
Vol 96 (4) ◽  
pp. 1538-1544 ◽  
Author(s):  
Anil Mishra ◽  
Simon P. Hogan ◽  
Eric B. Brandt ◽  
Marc E. Rothenberg
Keyword(s):  
Gastrointestinal Tract ◽  
Lamina Propria ◽  
Allergen Exposure ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Outer Cortex ◽  
Interleukin 5 ◽  
Marked Accumulation ◽  
Low Levels

Abstract The gastrointestinal immune system is traditionally thought to be composed of lymphocytes located within Peyer's patches and the lamina propria. We have recently reported that eosinophils also reside in the gastrointestinal tract during healthy states, in particular, within the lamina propria, and that these cells substantially increase after oral allergen exposure. We now demonstrate the presence of eosinophils in Peyer's patches and characterize the signals that regulate the accumulation of eosinophils in Peyer's patches. In contrast to the lamina propria, intestinal Peyer's patches have very low levels of eosinophils under healthy states. However, elevated levels of interleukin-5 (IL-5), generated by transgenic or pharmacologic approaches, result in a dramatic increase in eosinophil levels in Peyer's patches. Most eosinophils are located in the outer cortex and interfollicular regions of the Peyer's patches. To dissect the mechanism of eosinophil trafficking to Peyer's patches, the role of eotaxin was examined. Mice transgenic for IL-5 and genetically deficient in eotaxin were found to have reduced levels of eosinophils in Peyer's patches compared with IL-5-transgenic mice. To prove that eosinophils also traffic to Peyer's patches in wild-type mice, allergic hypersensitivity was induced and Peyer's patches were examined. Exposure to mucosal allergen promoted marked accumulation of eosinophils in Peyer's patches and this process was attenuated in eotaxin-deficient mice. In summary, these data demonstrate that elevated levels of IL-5 and mucosal allergen exposure promote eotaxin-dependent eosinophil trafficking to Peyer's patches. These studies suggest that eosinophils may cooperate with lymphocytes in the development of mucosal immune responses in the gastrointestinal tract.

scholarly journals Effect of Chronic Consumption of Sweeteners on Microbiota and Immunity in the Small Intestine of Young Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-16 ◽  
Author(s):  
B. E. Martínez-Carrillo ◽  
C. A. Rosales-Gómez ◽  
N. Ramírez-Durán ◽  
A. A. Reséndiz-Albor ◽  
J. A. Escoto-Herrera ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lamina Propria ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Anthropometric Parameters ◽  
C Peptide ◽  
Treatment Groups ◽  
Obesity And Diabetes ◽  
Group B ◽  
Young Mice

The consumption of sweeteners has increased as a measure to reduce the consumption of calories and thus combat obesity and diabetes. Sweeteners are found in a large number of products, so chronic consumption has been little explored. The objective of the study was to evaluate the effect of chronic sweetener consumption on the microbiota and immunity of the small intestine in young mice. We used 72 CD1 mice of 21 days old, divided into 3 groups: (i) No treatment, (ii) Group A (6 weeks of treatment), and (iii) Group B (12 weeks of treatment). Groups A and B were divided into 4 subgroups: Control (CL), Sucrose (Suc), Splenda® (Spl), and Svetia® (Sv). The following were determined: anthropometric parameters, percentage of lymphocytes of Peyer’s patches and lamina propria, IL-6, IL-17, leptin, resistin, C-peptide, and TNF-α. From feces, the microbiota of the small intestine was identified. The BMI was not modified; the mice preferred the consumption of Splenda® and Svetia®. The percentage of CD3+ lymphocytes in Peyer’s patches was increased. In the lamina propria, Svetia® increased the percentage of CD3+ lymphocytes, but Splenda® decreases it. The Splenda® and Svetia® subgroups elevate leptin, C-peptide, IL-6, and IL-17, with reduction of resistin. The predominant genus in all groups was Bacillus. The chronic consumption of sweeteners increases the population of lymphocytes in the mucosa of the small intestine. Maybe, Bacillus have the ability to adapt to sweeteners regardless of the origin or nutritional contribution of the same.

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