Assembly and Maturation of the Drosophila Larval Neuromuscular Junction

1999 ◽  
Vol 43 ◽  
pp. 93-117 ◽  
Author(s):  
L GRAMATES ◽  
V BUDNIK
Keyword(s):  
Neuromuscular Junction ◽  
Larval Neuromuscular Junction

scholarly journals Input-Specific Plasticity and Homeostasis at the Drosophila Larval Neuromuscular Junction

Neuron ◽  
2017 ◽  
Vol 93 (6) ◽  
pp. 1388-1404.e10 ◽  
Author(s):  
Zachary L. Newman ◽  
Adam Hoagland ◽  
Krishan Aghi ◽  
Kurtresha Worden ◽  
Sabrina L. Levy ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Larval Neuromuscular Junction

Quantal Unit Populations at the DrosophilaLarval Neuromuscular Junction

1999 ◽  
Vol 82 (3) ◽  
pp. 1497-1511 ◽  
Author(s):  
K. Wong ◽  
S. Karunanithi ◽  
H. L. Atwood
Keyword(s):  
Neuromuscular Junction ◽  
Time Course ◽  
Extracellular Recording ◽  
Intracellular Recordings ◽  
Quantal Size ◽  
Extracellular Signal ◽  
Spontaneous Frequency ◽  
The Individual ◽  
High Level ◽  
Larval Neuromuscular Junction

Focal extracellular recording at visualized boutons of the Drosophila larval neuromuscular junction was used to determine frequency and time course of the spontaneously occurring quantal events. When simultaneous intracellular recordings from the innervated muscle cell were made, more than one class of quantal event occurred at some of the individual boutons. “True” signals (arising at the bouton within the focal macropatch electrode) were often contaminated by additional signals generated outside the lumen of the focal electrode. Inclusion of these contaminating signals gave spuriously low values for relative amplitude, and spuriously high values for spontaneous quantal emission, for the synapses within the focal electrode. The contaminating signals, which appeared to be conducted along the subsynaptic reticulum surrounding the nerve terminals, generally were characterized by relatively small extracellular signals associated with normal intracellular events in the muscle fiber. From plots of simultaneous extracellular and intracellular recordings, the individual data points were classified according to the angles they subtended with the x axis (extracellular signal axis). Statistical procedures were developed to separate the true signals and contaminants with a high level of confidence. Populations of quantal events were found to be well described by Gaussian mixtures of two or three components, one of which could be characterized as the true signal population. Separation of signals from contaminants provides a basis for improving the estimates of quantal size and spontaneous frequency for the synapses sampled by the focal extracellular electrode.

scholarly journals Antagonistic Regulation of Circadian Output and Synaptic Development by the E3 Ubiquitin Ligase JETLAG and the DYSCHRONIC-SLOWPOKE Complex

2019 ◽  
Author(s):  
Angelique Lamaze ◽  
James E.C Jepson ◽  
Oghenerukevwe Akpoghiran ◽  
Kyunghee Koh
Keyword(s):  
Neuromuscular Junction ◽  
Ubiquitin Ligase ◽  
Light Exposure ◽  
E3 Ubiquitin Ligase ◽  
List Type ◽  
Cellular Functions ◽  
Synaptic Development ◽  
Channel Expression ◽  
Pdz Protein ◽  
Larval Neuromuscular Junction

SummaryCircadian output genes act downstream of the clock to promote rhythmic changes in behavior and physiology, yet their molecular and cellular functions are not well understood. Here we characterize an interaction between regulators of circadian entrainment, output and synaptic development in Drosophila that influences clock-driven anticipatory increases in morning and evening activity. We previously showed the JETLAG (JET) E3 Ubiquitin ligase resets the clock upon light exposure, while the PDZ protein DYSCHRONIC (DYSC) regulates circadian locomotor output and synaptic development. Surprisingly, we find that JET and DYSC antagonistically regulate synaptic development at the larval neuromuscular junction, and reduced JET activity rescues arrhythmicity of dysc mutants. Consistent with our prior finding that DYSC regulates SLOWPOKE (SLO) potassium channel expression, jet mutations also rescue circadian and synaptic phenotypes in slo mutants. Collectively, our data suggest that JET, DYSC and SLO promote circadian output in part by regulating synaptic morphology.HighlightsLoss of DYSC differentially impacts morning and evening oscillatorsReduced JET activity rescues the dysc and slo arrhythmic phenotypeReduced JET activity causes synaptic defects at the larval NMJJET opposes DYSC and SLO function at the NMJ synapse

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