Response of Seed Longevity to Moisture Content in Three Genotypes of Soyabean (GLYCINE MAX)

1993 ◽  
Vol 29 (4) ◽  
pp. 449-459 ◽  
Author(s):  
G. N. Zanakis ◽  
R. H. Ellis ◽  
R. J. Summerfield
Keyword(s):  
Glycine Max ◽  
Moisture Content ◽  
Seed Viability ◽  
Seed Storage ◽  
Seed Longevity ◽  
Seed Moisture Content ◽  
Significant Difference ◽  
The Cross ◽  
Equilibrium Relative Humidity ◽  
Logarithmic Relation

SummaryReports that an Indonesian soyabean (Glycine max) line (TGm737p) shows greater seed storage longevity than an American cultivar (Bossier), and that the cross between them (TG×536–02D) is intermediate between the two parents in this regard, were investigated by growing all three genotypes under identical conditions (30°C/20°C, 12 h d-1 photoperiod) in a plastics house. Seeds were harvested when moisture content had declined naturally to 14–15% (wet basis), and then stored hermetically at five different moisture contents at 40°C. Analyses of the resultant seed survival curves revealed that the seed lot constant Ki (a measure of potential longevity) was greatest in TGm737p and least in cv. Bossier, the cross being much closer to the American parent in this regard. The negative logarithmic relation between σ (standard deviation of the frequency distribution of seed deaths in time) and moisture content did not differ (p > 0.10) among the three genotypes. Moreover, this relation was close to that predicted by earlier results for four different genotypes. Similarly, there was no significant difference (p > 0.25) among genotypes in the negative semi-logarithmic relation between σ and seed equilibrium relative humidity (rh): the regression slope was equivalent to a doubling of longevity for each 8.0% reduction in rh. Finally, comparison of the negative logarithmic relation between absolute longevity (50% viability period) and seed moisture content confirmed that TGm737p showed greatest longevity (p > 0.005), but failed to show a difference in absolute longevity between cv. Bossier and the cross (p > 0.25). The results confirm that the seed viability equation of Ellis and Roberts (1980a, b) provides a framework within which the seed longevity of different genotypes can be compared. They suggest too that there is considerable doubt as to whether or not the greater potential longevity of the Indonesian line TGm737p has been incorporated successfully into the cross TG×536–02D.Longevidad de la semilla de soja

scholarly journals Primed Lettuce Seeds Exhibit Increased Sensitivity to Moisture Content During Controlled Deterioration

HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1436-1439 ◽  
Author(s):  
H.J. Hill ◽  
Jesse D. Cunningham ◽  
Kent J. Bradford ◽  
A.G. Taylor
Keyword(s):  
Moisture Content ◽  
Elevated Temperatures ◽  
Seed Viability ◽  
Seed Storage ◽  
Seed Priming ◽  
Storage Conditions ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Lettuce Seeds ◽  
Rate Of Decline

The Ellis-Roberts seed viability equation is used to predict seed survival after storage at specified temperatures and moisture contents. Seed priming, which can break dormancy and accelerate germination, can also reduce seed storage life. Because primed seeds were not used in developing the Ellis-Roberts equation, the reciprocal nature of specific seed moisture content (MC, fresh weight basis) and temperatures that applies to nonprimed lettuce (Lactuca sativa L.) seeds may not apply to primed seeds. To determine how priming affects lettuce seeds in relation to the viability equation, an experiment was conducted using two cultivars, ‘Big Ben’ and ‘Parris Island Cos’. Seeds primed in polyethylene glycol 8000 (–1.45 MPa, 24 h at 15 °C) and nonprimed seeds were first adjusted to 6% and 9% moisture contents and then stored at 48 and 38 °C for up to 30 days, respectively. These storage conditions (6% MC and 48 °C; 9% MC and 38 °C) were predicted by the viability equation to result in equal longevities. Subsequent viability assays at 20 °C revealed that nonprimed seeds in both storage environments exhibited similar losses in viability over time, thus validating the Ellis-Roberts equation and the use of these conditions to apply different but equal aging stress. Primed seeds of both cultivars deteriorated faster than nonprimed seeds as expected. However, primed seeds did exhibit different rates of deterioration between the storage environments. Primed seeds stored at 9% MC and 38 °C deteriorated faster than primed seeds stored at 6% MC and 48 °C. The rate of decline in probit viability percentage was three times greater in primed ‘Big Ben’ seeds stored at 9% MC and 38 °C than for those stored at 6% MC and 48 °C (–1.34 versus –0.26 probits per day, respectively). ‘Parris Island Cos’ seeds stored at 9% MC and 38 °C had twice the rate of deterioration that those stored at 6% MC and 48 °C (–1.19 and –0.49 probits per day, respectively). The results indicate that primed lettuce seeds were more sensitive to the adverse effects of higher seed MC than were nonprimed seeds during storage at elevated temperatures.

scholarly journals Model Dinamik Vigor Daya Simpan Benih Kedelai pada Penyimpanan Terbuka

2016 ◽  
Vol 34 (3) ◽  
pp. 219
Author(s):  
Ari Wahyuni ◽  
M. R. Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Initial Moisture Content ◽  
Seed Viability ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Input Model ◽  
Seed Storability

<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>

scholarly journals Effect of Desiccation and Storage Environment on Longevity of Ehretia Cymosa Thonn. Seeds.

2020 ◽  
Author(s):  
Peter Murithi Angaine ◽  
Stephen Muriithi Ndungú ◽  
Alice Adongo Onyango ◽  
Jesse Omondi Owino
Keyword(s):  
Moisture Content ◽  
Storage Temperature ◽  
Seed Viability ◽  
Seed Storage ◽  
Storage Duration ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Storage Behavior ◽  
Storage Studies ◽  
And Storage

Abstract Background: Globally, forestry faces challenges in the availability of seeds due to limited knowledge on seed handling of various species. Forestry seeds are constantly being reviewed and classified as either recalcitrant, intermediate, or orthodox based on their storage behavior. It is essential to understand the tree seed storage behavior to maintain seed viability and thus minimize seed losses. There is scanty literature combining factors of seed moisture content (6%, 9%, 12%, 15%, and 20%), seed storage temperature (20oC, 5oC and -20oC), seed storage duration (1, 4, 9 and 12 months), and germination in different sites with varying environmental variables. Ehretia cymosa is important in the Afromontane forestry landscape as a medicinal, rehabilitation, and conservation species. This study conducted desiccation and storage studies and their influence on the viability of E. cymosa seeds. The study sought to determine the optimum conditions for the storage of Ehretia cymosa that maintains viability. Results: This study observed that E. cymosa dried to seed moisture content of 6%, stored for 12 months at 20oC and sown in the laboratory had the highest germination performance (27.6 ± 3.18%) (p<0.05). Conclusion: This confirms that E.cymosa seeds exhibit orthodox storage behavior. The authors recommend longer storage studies (>12months) to determine the actual longevity of the seeds of this species. The significance of these results would be useful for foresters and farmers that would need to use this species for various purposes.

scholarly journals Effect of Periods and Media Storage on Viability of Cananga Seed (Cananga odorata (Lam.) Hook.f. & Thomson forma genuina)

2019 ◽  
Vol 7 (3) ◽  
pp. 277
Author(s):  
Ferawati Oktia Nurhayani ◽  
Arum Sekar Wulandari
Keyword(s):  
Seed Viability ◽  
Seed Storage ◽  
Maximum Growth ◽  
Germination Percentage ◽  
Growth Potential ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Significant Difference ◽  
Media Storage ◽  
Cananga Odorata

The suitable seed storage method is important to maintain seed viability. This study aimed to examine the effect of periods and media storage on the viability of cananga seed (Cananga odorata (Lam.) Hook.f. & Thomson forma genuina). The treatments used in the seed viability test were 0, 2, 4, and 6 weeks as period storage and cocopeat, ash, and charcoal as media storage. The seeds were stored in the plastic at room temperature (± 25°C). The variables observed in this study were seed moisture content, percentage of germination, maximum growth potential, average days of germination, T50, germination value, and the number of seeds that germinate during storage. The percentage of C. odorata seeds germination without storage is 54%. The seed storage for 2 – 6 weeks in ash and charcoal hush caused their viability was drop. The seeds storage of C. odorata up to 6 weeks in cocopeat could maintain seeds viability with a percentage of germination that has no significant difference compared to control. In general, cocopeat can be used as one of the storage media in the seed storage of C. odorata before sowing.Keywords: Cananga odorata, cocopeat, germination percentage, seed, viability

scholarly journals Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

2021 ◽  
Author(s):  
Filippo Guzzon ◽  
Maraeva Gianella ◽  
Jose Alejandro Velazquez Juarez ◽  
Cesar Sanchez Cano ◽  
Denise E Costich
Keyword(s):  
Seed Germination ◽  
Plant Genetic Resources ◽  
Seed Viability ◽  
Storage Conditions ◽  
Seed Longevity ◽  
Germplasm Bank ◽  
Significant Difference ◽  
Different Temperatures ◽  
Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

The effects of priming on seed longevity in Ranunculus sceleratus L.

1991 ◽  
Vol 1 (4) ◽  
pp. 243-249 ◽  
Author(s):  
R. J. Probert ◽  
S. V. Bogh ◽  
A. J. Smith ◽  
G. E. Wechsberg
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Ecological Factors ◽  
Fold Increase ◽  
Seed Longevity ◽  
Distilled Water ◽  
Survival Curves ◽  
Saturated Atmosphere ◽  
Equilibrium Relative Humidity ◽  
Species Specific

AbstractPriming in polyethylene glycol (PEG, −1.5 MPa) for 7 d followed by drying, led to a 4–5 fold increase in mean longevity when achenes (seeds) of Ranunculus sceleratus L. were subsequently stored at 35°C and 9.2% moisture content on a fresh weight basis (67.1% equilibrium relative humidity). The increase in longevity was due to an increase in the standard deviation of the frequency distribution of individual seed lifespans (decrease in the slope of transformed survival curves) and to a lesser extent to an increase in the intercept of survival curves. Priming for 1 d resulted in a smaller but significant increase in longevity independent of whether seeds were primed in PEG, distilled water or a saturated atmosphere (100% RH).The effects of priming were not due to the prevention of imbibition injury, and there was no evidence that the water relations of whole achenes was affected by priming. The effects of a 7 d priming treatment were dependent on the temperature and nature of the priming medium. However, no differences were recorded between corresponding treatments which were primed in PEG or distilled water. Moreover, the survival and responsivity to priming of R. sceleratus seeds was not related to dormancy status. The effects of priming on the longevity of R. sceleratus seeds are apparently unrelated to seed quality as the response of seeds previously aged for 8 d at 35°C and 7.9% moisture content was similar to that for unaged seeds.Priming followed by drying did not increase seed longevity in the related species R. acris L. The promotive effects of priming on seed survival appear to be species specific and may be related to ecological factors.

Seed storage behaviour of 101 woody species from the tropical rainforest of southern China: a test of the seed-coat ratio–seed mass (SCR–SM) model for determination of desiccation sensitivity

2014 ◽  
Vol 62 (4) ◽  
pp. 305 ◽  
Author(s):  
Qin-ying Lan ◽  
Ke Xia ◽  
Xiao-feng Wang ◽  
Jun-wei Liu ◽  
Jin Zhao ◽  
...  
Keyword(s):  
Moisture Content ◽  
Seed Coat ◽  
Tropical Rainforest ◽  
Southern China ◽  
Seed Mass ◽  
Seed Storage ◽  
Woody Species ◽  
Seed Moisture Content ◽  
Seed Desiccation ◽  
Desiccation Sensitivity

The Xishuangbanna tropical rainforest in Yunnan Province is the greatest biodiversity hotspot in China. However, the biodiversity of this region is under threat, making seed conservation through seed and/or germplasm banking particularly urgent and crucial. Seed desiccation sensitivity limits the possibility of seed banking of 47% of tropical rainforest species. Thus, knowing if a species has desiccation-sensitive seeds is an important first step in seed banking; however, often resources are limited, making it difficult to determine storage behaviour for all the species in a region. Prediction of seed sensitivity using the SCR–SM model based on seed-coat ratio (SCR) and seed dry mass (SM) might be an alternative for determining desiccation sensitivity of seeds of each species. Here, seed-desiccation sensitivity of 101 woody species from the Xishuangbanna tropical forest were analysed using this model, and physiological determinations were made for a total of 25 species. Seed storage behaviour for 59 species was used for model validation, and storage behaviour of 88% of these species was successfully predicted. Seed storage behaviour of 83% of the 59 species was successfully predicted using the 1000-seed weigth–moisture content (TSW–MC) criteria, which include seeds with 1000-seed weight >500 g and seed moisture content at shedding of 30 –70%. The two predictive methods were subsequently used to predict seed desiccation sensitivity for another 42 species from Xishuangbanna whose storage behaviour was uncertain. Our results indicated that ~50% of the species in Xishuangbanna are likely to have desiccation-sensitive seeds.

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