Redescription and synonymization of Oscheius citri Tabassum, Shahina, Nasira and Erum, 2016 (Rhabditida, Rhabditidae) from India and its taxonomical consequences

2021 ◽  
Vol 95 ◽  
Author(s):  
A. Rana ◽  
A.H. Bhat ◽  
A.K. Chaubey ◽  
V. Půža ◽  
J. Abolafia
Keyword(s):  
Indian Population ◽  
Uttar Pradesh ◽  
Nematode Species ◽  
Molecular Data ◽  
Molecular Characteristics ◽  
Morphometric Characteristics ◽  
High Similarity ◽  
Rdna Sequences ◽  
Indian Strain ◽  
28S Rdna Sequences

Abstract A population of a nematode species belonging to the genus Oscheius was isolated in western Uttar Pradesh, India. Morphological and morphometrical studies on this species showed its high similarity with six species described previously from Pakistan (Oscheius citri, O. cobbi, O. cynodonti, O. esculentus, O. punctatus and O. sacchari). The molecular analysis of the ITS1-5.8S-ITS2 rDNA sequences of the Indian population and the six species described from Pakistan showed that all the sequences are almost identical. Thus, based on morphological and molecular characteristics, all of the six above-mentioned Pakistani species and Indian strain do not differ from each other, hence can be considered synonyms. The correct name for this taxon is the first described species O. citri. Additionally, the phylogenetic analysis of the 18S rDNA and the 28S rDNA sequences showed that Oscheius citri is sister to the clade formed by O. chongmingensis and O. rugaoensis from China. The high similarity of morphological and morphometric characteristics of O. citri and other species, O. maqbooli, O. nadarajani, O. niazii, O. shamimi and O. siddiqii, suggest their conspecificity; however, lack of molecular data for these species does not allow this hypothesis to be tested.

scholarly journals Morphological, Morphometrical and Molecular Characterization of Oscheius siddiqii Tabassum and Shahina, 2010 (Rhabditida, Rhabditidae) from India with Its Taxonomic Consequences for the Subgenus Oscheius Andrássy, 1976

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1239
Author(s):  
Aashaq Hussain Bhat ◽  
Swati Gautum ◽  
Aasha Rana ◽  
Ashok Kumar Chaubey ◽  
Joaquín Abolafia ◽  
...  
Keyword(s):  
Agricultural Soils ◽  
Phylogenetic Analyses ◽  
Uttar Pradesh ◽  
Molecular Data ◽  
Morphological Data ◽  
Rdna Sequences ◽  
Morphological Studies ◽  
The Status ◽  
28S Rdna Sequences

An insect parasitic nematode belonging to the genus Oscheius was recovered from the agricultural soils from the Hapur district in western Uttar Pradesh, India. Morphological studies on this species exhibited its high resemblance with two Pakistani species: Oscheius siddiqii and O. niazii. No molecular data are available for these taxa but, morphologically, both species do not differ significantly from our strains and each other. Hence, these nematodes can be considered conspecific, and the correct name for this taxon is O. siddiqii, the first described species. The phylogenetic analyses of the ITS-, 18S-, and the 28S rDNA sequences showed that O. siddiqii is a sister taxon to the group formed by Oscheius microvilli, O. myriophilus, O. safricanus, and several unidentified Oscheius species. Additionally, our analyses show that based on molecular and morphological data, the species Oscheius rugaoensis and O. microvilli cannot be distinguished from O. chongmingensis and O. myriophilus, respectively, and are thus considered junior synonyms of these taxa. Furthermore, the available data are not sufficient to evaluate the status of Oscheius basothovii and O. safricanus, which are, in consequence, considered species inquirendae. These findings highlight the necessity of the proper morphological and molecular characterisation of the described Oscheius species.

Variable expression of molecular markers in juvenile nasopharyngeal angiofibroma

2017 ◽  
Vol 131 (9) ◽  
pp. 752-759 ◽  
Author(s):  
A Mishra ◽  
A Pandey ◽  
S C Mishra
Keyword(s):  
Molecular Markers ◽  
Clinical Characteristics ◽  
Indian Population ◽  
Molecular Data ◽  
Juvenile Nasopharyngeal Angiofibroma ◽  
Molecular Characteristics ◽  
Nasopharyngeal Angiofibroma ◽  
Variable Expression ◽  
Geographical Bias ◽  
Research Questions

AbstractBackground:Molecular categorisation may explain the wide variation in the clinical characteristics of juvenile nasopharyngeal angiofibroma.Methods:Variations in molecular markers in juvenile nasopharyngeal angiofibroma in an Indian population were investigated and compared with global reports.Results:Variable molecular marker expression was demonstrated at the regional and global levels. A wide variation in molecular characteristics is evident. Molecular data have been reported for only 11 countries, indicating a clear geographical bias. Only 58 markers have been studied, and most are yet to be validated.Conclusion:Research into the molecular epidemiology of juvenile nasopharyngeal angiofibroma is still in its infancy. Although the molecular variation is not well understood, data obtained so far have prompted important research questions. Hence, multicentre collaborative molecular studies are needed to establish the aetiopathogenesis and establish molecular surrogates for clinical characteristics.

Powdery mildew caused by Erysiphe lespedezae (including Pseudoidium caesalpiniacearum, syn. nov.) on Bauhinia blakeana and B. purpurea in China

Phytotaxa ◽  
2018 ◽  
Vol 345 (1) ◽  
pp. 35 ◽  
Author(s):  
BIAO XU ◽  
UWE BRAUN ◽  
SHANHE ZHANG ◽  
HUI YANG ◽  
ZHI CAO ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Description ◽  
New Host ◽  
Rdna Sequences ◽  
Powdery Mildews ◽  
Molecular Phylogenetic ◽  
28S Rdna Sequences

Bauhinia species are popular ornamental and medicinal plants with a pantropical distribution. In December 2016, powdery mildew symptoms were found on B. blakeana and B. purpurea in Guangdong, China. Based on ITS and 28S rDNA sequences, as well as morphological characters, the powdery mildew was identified as Erysiphe lespedezae. Previous records of powdery mildews on Bauhinia spp. are discussed. Based on morphological and molecular phylogenetic analyses of type material, Pseudoidium caesalpiniacearum is reduced to synonymy with E. lespedezae. To our best knowledge, this is the first report of E. lespedezae causing powdery mildew on B. purpurea in China, and B. blakeana as a new host. A detailed morphological description and molecular data are provided herein.

scholarly journals Phasmarhabditis zhejiangensis sp. nov. (Nematoda: Rhabditidae), a new rhabditid nematode from Zhejiang, China

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241413
Author(s):  
Chao-nan Zhang ◽  
Qi-zhi Liu
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Nematode Species ◽  
The Body ◽  
Molecular Analyses ◽  
Spicule Length ◽  
Rdna Sequences ◽  
Tail Shape ◽  
28S Rdna Sequences ◽  
A New Species

A new nematode species of the genus Phasmarhabditis was isolated from the body surface of a slug (Philomycus bilineatus Benson, PB). Morphological and molecular analyses confirmed this nematode as a new species. The nematode was named Phasmarhabditis zhejiangensis sp. nov. (Nematoda: Rhabditidae) and is dioecious. In males, the open bursa with genital papillae is characterized by the formula 1-1-1-2-1-3, and the spicule length is 58μm. In female, the vulva is located approximately in the middle of the body. The nematode belongs to papillosa group because of its tail shape pointed with filiform tip. The phasmids are rod-shaped. The posterior anus is slightly swollen. P. zhejiangensis was further characterized by internal transcribed spacer (ITS), 18S rDNA and 28S rDNA sequences. After the sequencing results were compared with sequences available from the National Center for Biotechnology Information (NCBI), the maximum similarities of ITS, 18S and 28S sequences were 89.81%, 96.22% and 95.28%, respectively. Phylogenetic analyses placed Phasmarhabditis zhejiangensis sp. nov. in the genus Phasmarhabditis.

Neoalbatrellus odorus sp. nov. (Albatrellaceae, Russulales) from Southwest China

Phytotaxa ◽  
2017 ◽  
Vol 309 (3) ◽  
pp. 217 ◽  
Author(s):  
YUAN-YUAN CHEN ◽  
MIN WANG ◽  
BO ZHANG ◽  
BAO-KAI CUI
Keyword(s):  
New Species ◽  
Bayesian Inference ◽  
Maximum Likelihood ◽  
Southwest China ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Pore Surface ◽  
Rdna Sequences ◽  
Dark Violet ◽  
28S Rdna Sequences

We describe herein the Neoalbatrellus odorus sp. nov. accounting on its peculiar morphological features and molecular data. It is characterized by fleshy basidiomata with a deep violet to dark violet pileal surface, which turns blackish blue and glossy after drying. It also has a white pore surface, light violet to bluish violet stipe, simple septate generative hyphae, and thick-walled, non-amyloid basidiospores. The phylogenetic analyses, based on ITS and 28S rDNA sequences of Neoalbatrellus and its related genera, were performed using Maximum Parsimony, Maximum Likelihood and Bayesian Inference, confirming the affiliation of the new species to Neoalbatrellus.

Redescription of Acanthogyrus (Acanthosentis) maroccanus (Dollfus, 1951) (Acanthocephala: Quadrigyridae), a parasite of the Algerian barb Luciobarbus callensis (Valenciennes) (Cyprinidae) in Algeria, and first molecular data

2019 ◽  
Vol 94 ◽  
Author(s):  
A. Menasria ◽  
D. Barčák ◽  
N. Kaouachi ◽  
M. Bensouilah ◽  
T. Scholz ◽  
...  
Keyword(s):  
Bayesian Inference ◽  
Maximum Likelihood ◽  
Molecular Data ◽  
Morphological Features ◽  
Phylogenetic Position ◽  
Reproductive Structures ◽  
Rdna Sequences ◽  
Northwest Africa ◽  
28S Rdna Sequences ◽  
Cyprinid Fishes

Abstract Acanthogyrus (Acanthosentis) maroccanus (Dollfus, 1951), an insufficiently described quadrigyrid acanthocephalan of cyprinid fishes from Northwest Africa, is redescribed based on recently collected specimens from the Algerian barb Luciobarbus callensis (Valenciennes) in Algeria. Newly observed morphological features for A. (A.) maroccanus include the arrangement of proboscis hooks (not in regular circles), the male reproductive structures extending into the copulatory bursa and the presence of a para-receptacle structure and vaginal sleeve. The mechanism of copulation of this acanthocephalan is described based on several copulating pairs. The phylogenetic position of A. (A.) maroccanus within Eoacanthocephala was assessed based on partial 28S rDNA sequences. Maximum likelihood and Bayesian inference analyses placed A. (A.) maroccanus in a clade with Palliolisentis (Demidueterospinus) ophiocephalus (Thapar, 1931), both species included in the Quadrigyridae, the only family within the Gyracanthocephala.

scholarly journals Taxonomic resolution of Stylonychia notophora (Alveolata, Ciliophora, Hypotricha) sensu Sapra and Dass 1970 using an integrative approach

2020 ◽  
Vol 70 (6) ◽  
pp. 3939-3952
Author(s):  
Harpreet Kaur ◽  
Shashi ◽  
Alan Warren ◽  
Ram Krishan Negi ◽  
Komal Kamra
Keyword(s):  
18S Rdna ◽  
Species Delimitation ◽  
Indian Population ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Taxonomic Resolution ◽  
Integrative Approach ◽  
Rdna Sequences ◽  
18S Rdna Sequences ◽  
River Yamuna

The spirotrichean ciliate Stylonychia notophora has previously been recorded in India although the descriptions are lacking in detail. It has been suggested several times that the Indian population, S. notophora sensu Sapra and Dass, 1970 collected along the Delhi stretch of the River Yamuna, is identical to Tetmemena pustulata, but this has never been confirmed due to insufficient data for the former. The present study includes detailed descriptions (classical and molecular) of populations of Tetmemena isolated from six locations along the River Yamuna, India. These include four from the Delhi stretch including that from which Sapra and Dass, 1970 isolated their population of S. notophora. Due to the lack of a sufficiently detailed description, the taxonomic status of S. notophora sensu Sapra and Dass, 1970 was not clear. Comparisons among the populations isolated in the present study with previous descriptions of T. pustulata and S. notophora sensu Sapra and Dass, 1970 show only minor differences in morphometry, morphogenesis and in 18S rDNA sequences. The 18S rDNA sequences of all six populations had 99% similarity to both T. pustulata and S. notophora. These findings support the contention that S. notophora sensu Sapra and Dass, 1970 was misidentified and is a population of T. pustulata. This study supports the need for adopting an integrative approach based on morphological, morphogenetic and molecular data in order to understand species delimitation in ciliated protists.

Morphological and molecular characterisation of Neometadorylaimus coomansi (Ali, Farooqui & Suryawanshi, 1971) Jairajpuri & Ahmad, 1992 (Dorylaimida: Tylencholaimidae), with the first report of its male and new insights into its phylogeny

Nematology ◽  
2020 ◽  
Vol 22 (3) ◽  
pp. 313-325
Author(s):  
Md Niraul Islam ◽  
Zarrin Imran ◽  
Joaquín Abolafia ◽  
Reyes Peña-Santiago ◽  
Wasim Ahmad
Keyword(s):  
West Bengal ◽  
Molecular Data ◽  
The Body ◽  
Integrative Approach ◽  
Molecular Characterisation ◽  
Its Phylogeny ◽  
Rdna Sequences ◽  
28S Rdna Sequences ◽  
Morphological And Molecular Data ◽  
Abundant Population

Summary The rare genus and species Neometadorylaimus coomansi is characterised based on an abundant population found in West Bengal, India. New morphological and morphometric data are provided, together with SEM observations and the first description of its male. Molecular analyses (18S and 28S-rDNA sequences) were also done. A comparison of the present population with the two previously known ones, all from India, showed a remarkable variability in some features, especially the body length that ranges from 1.75 to 3.29 mm. The male is distinguished by its 1.83-2.06 mm long body, 54-55 μm long spicules, and nine or ten ventromedian supplements lacking an hiatus. New insights into the phylogeny of the genus are provided with an integrative approach combining morphological and molecular data. The monophyly of the Tylencholaimidae is supported, and a tentative relationship between Tylencholaimidae and other dorylaims lacking the pars refringens vaginae is suggested.

scholarly journals Morphological and molecular characteristics of foliar nematode attacking silver birch (Betula pendula Roth) in Poland

2017 ◽  
Vol 54 (3) ◽  
pp. 250-256 ◽  
Author(s):  
A. Chałańska ◽  
A. Bogumił ◽  
G. Winiszewska ◽  
K. Kowalewska ◽  
T. Malewski
Keyword(s):  
Betula Pendula ◽  
Silver Birch ◽  
Molecular Characteristics ◽  
Perennial Plant ◽  
Source Of Infection ◽  
Rdna Sequences ◽  
Morphological Diagnosis ◽  
28S Rdna Sequences ◽  
Aphelenchoides Fragariae ◽  
Foliar Nematode

SummaryAphelenchoides fragariae (Ritzema Bos, 1890) Christie, 1932 was isolated from leaves of silver birch (Betula pendula Roth) seedlings proving that the source of infection was anemones plants. This is the first report to our best knowledge showing that the source of nematode infection of a woody plant could be a perennial plant. A. fragariae was identified by morphometric and molecular analyses. Morphological diagnosis based on the bending shape of the tail of males and pronounced apex and rostrum proved to be the most accurate reliable characteristic. On the opposite, the high variability of the mucron shape in female tails made the identification by microscopic analyses difficult. Identification of the species was confirmed by analysis of 28S rDNA sequences. The morphometric data of adults extracted from silver birch was compared with that of nematodes isolated from Anemone hupehensis (Lemoine) Lemoine. Males body length varied highly in samples collected from both host plant species.

Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family

Nematology ◽  
2016 ◽  
Vol 18 (8) ◽  
pp. 987-998 ◽  
Author(s):  
Mahya Soleymanzadeh ◽  
Majid Pedram ◽  
Ebrahim Pourjam ◽  
Sergio Álvarez-Ortega
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Molecular Data ◽  
Lateral View ◽  
Lateral Field ◽  
Rdna Sequences ◽  
Phylogenetic Studies ◽  
Sister Clade ◽  
The Family ◽  
28S Rdna Sequences

Lelenchus brevislitus n. sp. is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by having females with slender, 452-488 μm long, body lateral field vestigial as a plain simple band under scanning electron microscopy (SEM) in vulval region and absent in proximal and distal body ends, cephalic region short, ca 2 μm high, conical and truncate in anterior end in lateral view under SEM, dorso-ventrally flattened, amphidial pouches pocket-shaped and large under light microscopy (LM), their apertures short (ca 1 μm long in SEM) longitudinal sinuous slits remarkably wide near the small oral disc and significantly narrowing posteriad stylet, slender. 6.5-8.0 μm long, developed median bulb with distinct valvular plates, monodelphic-prodelphic reproductive system having a short post-vulval uterine sac (PUS), transverse vulval slit (V = 47.5-51.7) bearing small lateral membranes on each side, long filiform female tail (154-170 μm, c = 2.8-3.1, c′ = 21.5-28.3), and males unknown. The new species is considered to be a cryptic species inside the Tylenchidae. Morphological comparisons with all species of the genus are discussed. Phylogenetic studies were performed for further characterisation of the new species, and the results, using nearly full length 18S rDNA data, revealed it formed a clade with an isolate of L. leptosoma, both of which are in a sister clade with an isolate of Sakia. In a reconstructed phylogenetic tree using partial 28S rDNA sequences, the new species formed a monophyletic group, again with L. leptosoma, a sister clade to some Malenchus spp.

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