scholarly journals Correction to Single Virus Tracking with Quantum Dots Packaged into Enveloped Viruses Using CRISPR

Nano Letters ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 2931-2931
Author(s):  
Yong-Bo Yang ◽  
Yan-Dong Tang ◽  
Yue Hu ◽  
Fang Yu ◽  
Jun-Yao Xiong ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Enveloped Viruses ◽  
Single Virus Tracking

scholarly journals Lipid-Specific Labeling of Enveloped Viruses with Quantum Dots for Single-Virus Tracking

mBio ◽  
2020 ◽  
Vol 11 (3) ◽  
Author(s):  
Li-Juan Zhang ◽  
Shaobo Wang ◽  
Li Xia ◽  
Cheng Lv ◽  
Hong-Wu Tang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Optical Properties ◽  
Virus Infection ◽  
Quantum Dot ◽  
Single Molecule ◽  
Host Cells ◽  
Single Molecule Tracking ◽  
Enveloped Viruses ◽  
Single Virus Tracking ◽  
Labeling Method

ABSTRACT Quantum dots (QDs) possess optical properties of superbright fluorescence, excellent photostability, narrow emission spectra, and optional colors. Labeled with QDs, single molecules/viruses can be rapidly and continuously imaged for a long time, providing more detailed information than when labeled with other fluorophores. While they are widely used to label proteins in single-molecule-tracking studies, QDs have rarely been used to study virus infection, mainly due to a lack of accepted labeling strategies. Here, we report a general method to mildly and readily label enveloped viruses with QDs. Lipid-biotin conjugates were used to recognize and mark viral lipid membranes, and streptavidin-QD conjugates were used to light them up. Such a method allowed enveloped viruses to be labeled in 2 h with specificity and efficiency up to 99% and 98%, respectively. The intact morphology and the native infectivity of viruses were preserved. With the aid of this QD labeling method, we lit wild-type and mutant Japanese encephalitis viruses up, tracked their infection in living Vero cells, and found that H144A and Q258A substitutions in the envelope protein did not affect the virus intracellular trafficking. The lipid-specific QD labeling method described in this study provides a handy and practical tool to readily “see” the viruses and follow their infection, facilitating the widespread use of single-virus tracking and the uncovering of complex infection mechanisms. IMPORTANCE Virus infection in host cells is a complex process comprising a large number of dynamic molecular events. Single-virus tracking is a versatile technique to study these events. To perform this technique, viruses must be fluorescently labeled to be visible to fluorescence microscopes. The quantum dot is a kind of fluorescent tag that has many unique optical properties. It has been widely used to label proteins in single-molecule-tracking studies but rarely used to study virus infection, mainly due to the lack of an accepted labeling method. In this study, we developed a lipid-specific method to readily, mildly, specifically, and efficiently label enveloped viruses with quantum dots by recognizing viral envelope lipids with lipid-biotin conjugates and recognizing these lipid-biotin conjugates with streptavidin-quantum dot conjugates. It is not only applicable to normal viruses, but also competent to label the key protein-mutated viruses and the inactivated highly virulent viruses, providing a powerful tool for single-virus tracking.

scholarly journals Site-Specific Labeling of Enveloped Viruses with Quantum Dots for Single Virus Tracking

ACS Nano ◽  
2008 ◽  
Vol 2 (8) ◽  
pp. 1553-1562 ◽  
Author(s):  
Kye-Il Joo ◽  
Yuning Lei ◽  
Chi-Lin Lee ◽  
Jonathon Lo ◽  
Jiansong Xie ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Site Specific ◽  
Enveloped Viruses ◽  
Single Virus Tracking

scholarly journals Lipid-specific labeling of enveloped viruses with quantum dots for single-virus tracking

2020 ◽  
Author(s):  
Li-Juan Zhang ◽  
Shaobo Wang ◽  
Li Xia ◽  
Cheng Lv ◽  
Hong-Wu Tang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Optical Properties ◽  
Virus Infection ◽  
Quantum Dot ◽  
Single Molecule ◽  
Host Cells ◽  
Single Molecule Tracking ◽  
Enveloped Viruses ◽  
Single Virus Tracking ◽  
Labeling Method

AbstractQuantum dots (QDs) possess optical properties of superbright fluorescence, excellent photostability, narrow emission spectra, and optional colors. Labeled with QDs, single molecules/viruses can be rapidly and continuously imaged for a long time, providing more detailed information than labeled with other fluorophores. While they are widely used to label proteins in single-molecule tracking studies, QDs have rarely been used to study virus infection, mainly due to lack of accepted labeling strategies. Here, we report a general method to mildly and readily label enveloped viruses with QDs. Lipid-biotin conjugates were used to recognize and mark viral lipid membranes, and streptavidin (SA)-QD conjugates were used to light them up. Such a method allowed enveloped viruses to be labeled in 2 hours with specificity and efficiency up to 99% and 98%. The intact morphology and the native infectivity of viruses could be furthest preserved. With the aid of this QD labeling method, we lit wild-type (WT) and mutant Japanese encephalitis virus (JEV) up, tracked their infection in living Vero cells, and found that H144A and Q258A substitutions in the envelope (E) protein didn’t affect the virus intracellular trafficking. The lipid-specific QD labeling method described in this study provides a handy and practical tool to readily “see” the viruses and follow their infection, facilitating the widespread use of single-virus tracking and the uncovering of complex infection mechanisms.Author summaryVirus infection in host cells is a complex process comprising a large number of dynamic molecular events. Single-virus tracking is a versatile technique to study these events. To perform this technique, viruses must be fluorescently labeled to be visible to fluorescence microscopes. Quantum dot is a kind of fluorescent tags that has many unique optical properties. It has been widely used to label proteins in single-molecule tracking studies, but rarely used to study virus infection, mainly due to lack of accepted labeling method. In this study, we developed a lipid-specific method to readily, mildly, specifically, and efficiently label enveloped viruses with quantum dots by recognizing viral envelope lipids with lipid-biotin conjugates and recognizing these lipid-biotin conjugates with streptavidin-quantum dot conjugates. Such a method is superior to the commonly used DiD/DiO labeling and the other QD labeling methods. It is not only applicable to normal viruses, but also competent to label the key protein-mutated viruses and the inactivated high virulent viruses, providing a powerful tool for single-virus tracking.

Single Virus Tracking with Quantum Dots Packaged into Enveloped Viruses Using CRISPR

Nano Letters ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 1417-1427 ◽  
Author(s):  
Yong-Bo Yang ◽  
Yan-Dong Tang ◽  
Yue Hu ◽  
Fang Yu ◽  
Jun-Yao Xiong ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Enveloped Viruses ◽  
Single Virus Tracking

Labeling the nucleocapsid of enveloped baculovirus with quantum dots for single-virus tracking

Biomaterials ◽  
2014 ◽  
Vol 35 (7) ◽  
pp. 2295-2301 ◽  
Author(s):  
Li Wen ◽  
Yi Lin ◽  
Zhen-Hua Zheng ◽  
Zhi-Ling Zhang ◽  
Li-Juan Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Single Virus Tracking

scholarly journals Visualizing the Transport of Porcine Reproductive and Respiratory Syndrome Virus in Live Cells by Quantum Dots-Based Single Virus Tracking

2019 ◽  
Vol 35 (4) ◽  
pp. 407-416
Author(s):  
Zhenpu Liang ◽  
Pengjuan Li ◽  
Caiping Wang ◽  
Deepali Singh ◽  
Xiaoxia Zhang
Keyword(s):  
Quantum Dots ◽  
Live Cells ◽  
Respiratory Syndrome Virus ◽  
Single Virus Tracking

Encapsulating Quantum Dots within HIV-1 Virions through Site-Specific Decoration of the Matrix Protein Enables Single Virus Tracking in Live Primary Macrophages

Nano Letters ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 7457-7468 ◽  
Author(s):  
Qin Li ◽  
Wen Yin ◽  
Wei Li ◽  
Zhiping Zhang ◽  
Xiaowei Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Matrix Protein ◽  
Site Specific ◽  
The Matrix ◽  
Single Virus Tracking ◽  
Hiv 1

Tracking single viruses infecting their host cells using quantum dots

2016 ◽  
Vol 45 (5) ◽  
pp. 1211-1224 ◽  
Author(s):  
Shu-Lin Liu ◽  
Zhi-Gang Wang ◽  
Zhi-Ling Zhang ◽  
Dai-Wen Pang
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Host Cells ◽  
Meaningful Information ◽  
Single Virus Tracking

We describe the implementation of quantum dot-based single-virus tracking and show how to use this technique to acquire meaningful information.

Nanostructure of semiconductor quantum dots in a borosilicate glass matrix by complementary use of HREM and AEM

1990 ◽  
Vol 48 (4) ◽  
pp. 728-729
Author(s):  
M.J. Kim ◽  
L.C. Liu ◽  
S.H. Risbud ◽  
R.W. Carpenter
Keyword(s):  
Quantum Dots ◽  
Borosilicate Glass ◽  
Glass Matrix ◽  
Optical Switching ◽  
Response Times ◽  
Fast Response ◽  
Processing Technique ◽  
Internal Stresses ◽  
Electron Hole ◽  
Spatial Dimensions

When the size of a semiconductor is reduced by an appropriate materials processing technique to a dimension less than about twice the radius of an exciton in the bulk crystal, the band like structure of the semiconductor gives way to discrete molecular orbital electronic states. Clusters of semiconductors in a size regime lower than 2R {where R is the exciton Bohr radius; e.g. 3 nm for CdS and 7.3 nm for CdTe) are called Quantum Dots (QD) because they confine optically excited electron- hole pairs (excitons) in all three spatial dimensions. Structures based on QD are of great interest because of fast response times and non-linearity in optical switching applications.In this paper we report the first HREM analysis of the size and structure of CdTe and CdS QD formed by precipitation from a modified borosilicate glass matrix. The glass melts were quenched by pouring on brass plates, and then annealed to relieve internal stresses. QD precipitate particles were formed during subsequent "striking" heat treatments above the glass crystallization temperature, which was determined by differential thermal analysis.

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