Virus Specific Ribonucleic Acids in Baby Hamster Kidney Cells Infected With Foot-and-Mouth Disease Virus

Nature ◽  
1964 ◽  
Vol 204 (4961) ◽  
pp. 855-856 ◽  
Author(s):  
F. BROWN ◽  
B. CARTWRIGHT
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Ribonucleic Acids ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Baby Hamster Kidney Cells

scholarly journals A study of the heterogeneous 37s ribonucleic acid induced by foot-and-mouth-disease virus

1968 ◽  
Vol 107 (3) ◽  
pp. 395-401 ◽  
Author(s):  
T. F. Wild ◽  
S J Martin ◽  
F. Brown
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Virus Particles ◽  
Mouth Disease Virus ◽  
Heterogeneous Nature ◽  
Foot And Mouth ◽  
Sepharose 4B ◽  
Baby Hamster Kidney Cells

1. The 37s RNA induced in baby-hamster kidney cells by infection with foot-and-mouth-disease virus was examined on sucrose gradients and by filtration through Sepharose 4B. 2. The RNA sedimented faster (37s) and as a broader band than the 35s RNA from purified virus. 3. Treatment with deoxyribonuclease, Pronase or amylase did not alter the sedimentation profile of the 37s RNA. 4. Treatment of individual fractions of the RNA with phenol, dimethyl sulphoxide or methylCellosolve did not decrease the sedimentation rate of the faster-sedimenting molecules. 5. Sedimentation in sucrose gradients of different ionic strengths or containing EDTA had no effect on the heterogeneous nature of the profile. 6. On filtration through Sepharose 4B columns, the 37s virus-induced RNA was eluted before viral RNA. 7. Only 20% of the rapidly sedimenting RNA was incorporated into complete virus particles.

scholarly journals RELATIONSHIP OF DONOR AGE TO IN VITRO PRODUCTION OF FOOT-AND-MOUTH DISEASE VIRUS BY MOUSE KIDNEY CELLS

1965 ◽  
Vol 121 (1) ◽  
pp. 69-83 ◽  
Author(s):  
Charles H. Campbell
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Virus Multiplication ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Monolayer Cultures ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Primary Monolayer ◽  
Primary Monolayer Cultures

Multiplication of foot-and-mouth disease virus (FMDV) was compared in kidney cells from 7- to 35-day-old mice representing various degrees of age resistance to this virus. Three types of cell preparations were used: primary monolayer cultures, suspensions of dispersed cells, and suspensions of minced tissue. Virus multiplication in the two types of cell suspensions was related to the age of the donors both in regard to time when multiplication first became evident and to the amount of virus produced. While adsorption rates were similar in the cells from all age groups, virus multiplication began earlier in cells from younger mice and more virus was produced by these cells than by cells from older animals. There was no significant difference in the virus growth rates in the primary monolayer cultures of cells. The results indicate that kidney cells from mice 7 to 35 days old vary in their ability to produce virus in relation to the degree of susceptibility of the cell donors. After propagation of the cells in primary monolayer cultures, however, this difference no longer exists probably because of cell selection under the cultural conditions.

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