scholarly journals An integrated magneto-electrochemical device for the rapid profiling of tumour extracellular vesicles from blood plasma

2021 ◽  
Author(s):  
Jongmin Park ◽  
Jun Seok Park ◽  
Chen-Han Huang ◽  
Ala Jo ◽  
Kaitlyn Cook ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Electrochemical Device

scholarly journals Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions

2021 ◽  
Vol 10 (10) ◽  
Author(s):  
Glenn Vergauwen ◽  
Joeri Tulkens ◽  
Cláudio Pinheiro ◽  
Francisco Avila Cobos ◽  
Sándor Dedeyne ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Clinical Conditions

scholarly journals Prospects and limitations of antibody-mediated clearing of lipoproteins from blood plasma prior to nanoparticle tracking analysis of extracellular vesicles

2017 ◽  
Vol 6 (1) ◽  
pp. 1308779 ◽  
Author(s):  
Morten Mørk ◽  
Aase Handberg ◽  
Shona Pedersen ◽  
Malene M. Jørgensen ◽  
Rikke Bæk ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Nanoparticle Tracking Analysis

Elevated blood plasma levels of tissue factor-bearing extracellular vesicles in patients with atrial fibrillation

2019 ◽  
Vol 173 ◽  
pp. 141-150 ◽  
Author(s):  
Morten Mørk ◽  
Jan J. Andreasen ◽  
Lars H. Rasmussen ◽  
Gregory Y.H. Lip ◽  
Shona Pedersen ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Blood Plasma ◽  
Tissue Factor ◽  
Extracellular Vesicles ◽  
Plasma Levels ◽  
Elevated Blood

scholarly journals An miRNA fingerprint using neural-enriched extracellular vesicles from blood plasma: towards a biomarker for amyotrophic lateral sclerosis/motor neuron disease

Open Biology ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 200116 ◽  
Author(s):  
Sandra Anne Banack ◽  
Rachael Anne Dunlop ◽  
Paul Alan Cox
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Blood Plasma ◽  
Motor Neuron ◽  
Motor Neuron Disease ◽  
Extracellular Vesicles ◽  
Disease Diagnosis ◽  
Extracellular Vesicle ◽  
Improve Patient ◽  
Lateral Sclerosis

Biomarkers for amyotrophic lateral sclerosis/motor neuron disease (ALS/MND) are currently not clinically available for disease diagnosis or analysis of disease progression. If identified, biomarkers could improve patient outcomes by enabling early intervention and assist in the determination of treatment efficacy. We hypothesized that neural-enriched extracellular vesicles could provide microRNA (miRNA) fingerprints with unequivocal signatures of neurodegeneration. Using blood plasma from ALS/MND patients and controls, we extracted neural-enriched extracellular vesicle fractions and conducted next-generation sequencing and qPCR of miRNA components of the transcriptome. We here report eight miRNA sequences which significantly distinguish ALS/MND patients from controls in a replicated experiment using a second cohort of patients and controls. miRNA sequences from patient blood samples using neural-enriched extracellular vesicles may yield unique insights into mechanisms of neurodegeneration and assist in early diagnosis of ALS/MND.

scholarly journals Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1046 ◽  
Author(s):  
Božič ◽  
Sitar ◽  
Junkar ◽  
Štukelj ◽  
Pajnič ◽  
...  
Keyword(s):  
Light Scattering ◽  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Viscosity Coefficient ◽  
Plausible Model ◽  
Average Shape ◽  
Key Factor ◽  
Spherical Vesicles ◽  
In Blood Plasma

Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.

scholarly journals Evaluation of plasmatic extracellular vesicles by size

2021 ◽  
Author(s):  
Laura Cantone ◽  
Mirjam Hoxha ◽  
Chiara Favero ◽  
Luca Ferrari ◽  
Valentina Bollati
Keyword(s):  
Plasma Membrane ◽  
Blood Plasma ◽  
Extracellular Vesicles ◽  
High Precision ◽  
Extracellular Vesicle ◽  
Nanoparticle Tracking Analysis ◽  
Research Needs ◽  
Plasma Samples ◽  
Constant Flow ◽  
Syringe Pump

Abstract Extracellular vesicles (EVs) play a key role in many physiological and pathological processes [1]. EVs are a heterogeneous group of membrane-confined particles including endosome-derived exosomes and plasma membrane-originated microvesicles. The expanding field of extracellular vesicle research needs reproducible and accurate methods to characterize EVs [2]. EV profiling can be challenging due to the small size and heterogeneity. This protocol aims to provide a method to isolate EVs and facilitate high-precision particle quantitation by Nanoparticle Tracking Analysis (NTA)[3, 4]. NTA is commonly used to determine EV concentration and diameter [5, 6]. The protocol here described refers to the isolation of EVs from blood-plasma samples by using ultracentrifugation and then quantification and sizing of EVs with NTA by NanoSight NS300 system (Malvern Panalytical Ltd., Malvern, UK) provided with a syringe pump module enabling analysis in constant flow for improved sample statistics.

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