scholarly journals Crystal structure of MytiLec, a galactose-binding lectin from the mussel Mytilus galloprovincialis with cytotoxicity against certain cancer cell types

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Daiki Terada ◽  
Fumihiro Kawai ◽  
Hiroki Noguchi ◽  
Satoru Unzai ◽  
Imtiaj Hasan ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Cancer Cell ◽  
Mytilus Galloprovincialis ◽  
Cell Types ◽  
Binding Lectin ◽  
Galactose Binding Lectin

scholarly journals Multiple proteins related to the soluble galactose-binding animal lectin revealed by a monoclonal anti-lectin antibody

1985 ◽  
Vol 228 (1) ◽  
pp. 147-153 ◽  
Author(s):  
S R Carding ◽  
R A Childs ◽  
R Thorpe ◽  
M Spitz ◽  
T Feizi
Keyword(s):  
Solid Phase ◽  
Cell Types ◽  
Binding Activity ◽  
Active Components ◽  
Muscle Tissues ◽  
Bovine Tissue ◽  
Tissue Homogenates ◽  
Binding Lectin ◽  
Animal Lectin ◽  
Galactose Binding Lectin

A monoclonal antibody (NIBy 142-36/8) raised against the soluble galactose-binding lectin of bovine heart muscle has been tested by solid-phase vinyl-plate radiobinding and nitrocellulose immunoblotting with homogenates of various bovine tissues, and the muscle tissues of pig, rabbit, chicken and rat. Muscle lectins of chicken, rabbit and rat differed from those of man and pig in their lack of reactivity with the 36/8 antibody. There was a good correlation of haemagglutinating activities and immunoreactivities of the bovine tissue homogenates, suggesting that the soluble galactose-binding protein is a major haemagglutinin in various tissues. Immunoblotting experiments revealed an array of antigenically active components in the homogenates in addition to the 13 and 26kDa proteins that were previously detected in preparations of purified lectin. These were in the range 36kDa to more than 200kDa, and a different spectrum of immunoreactive components was found in various cell types. Galactose-binding activity was demonstrable in 13, 26 and 36kDa components in certain bovine tissues, suggesting that the immunoreactive components of higher Mr may be inactive precursor forms of the lectin.

Effect of Various Polyols on the Acid-Denatured States of Champedak Galactose-Binding Lectin

2018 ◽  
Vol 25 (3) ◽  
pp. 314-324 ◽  
Author(s):  
Nurul I.A. Kameel ◽  
Adawiyah S. Shuib ◽  
Saad Tayyab
Keyword(s):  
Denatured States ◽  
Binding Lectin ◽  
Galactose Binding Lectin

scholarly journals Inhibition of MMP2-PEX by a novel ester of dihydroxy cinnamic and linoleic acid from the seagrass Cymodocea serrulata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
V. S. Christina ◽  
R. Lakshmi Sundaram ◽  
V. Sivamurugan ◽  
D. Thirumal Kumar ◽  
C. D. Mohanapriya ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Ovarian Cancer Cell Line ◽  
Cancer Cell Line ◽  
Md Simulations ◽  
Cell Types ◽  
Structural Details ◽  
M Phase ◽  
Ft Ir ◽  
Target Binding ◽  
Cymodocea Serrulata

AbstractMatrix metalloproteinases (MMPs) are pivotal for cancer cell migration and metastasis which are generally over-expressed in such cell types. Many drugs targeting MMPs do so by binding to the conserved catalytic domains and thus exhibit poor selectivity due to domain-similarities with other proteases. We report herein the binding of a novel compound [3-(E-3,4-dihydroxycinnamaoyloxyl)-2-hydroxypropyl 9Z, 12Z-octadeca-9, 12-dienoate; Mol. wt: 516.67 Da], (C1), isolated from a seagrass, Cymodocea serrulata to the unconserved hemopexin-like (PEX) domain of MMP2 (− 9.258 kcal/mol). MD simulations for 25 ns, suggest stable ligand-target binding. In addition, C1 killed an ovarian cancer cell line, PA1 at IC50: 5.8 μM (lesser than Doxorubicin: 8.6 µM) and formed micronuclei, apoptotic bodies and nucleoplasmic bridges whilst causing DNA laddering, S and G2/M phase dual arrests and MMP disturbance, suggesting intrinsic apoptosis. The molecule increased mRNA transcripts of BAX and BAD and down-regulated cell survival genes, Bcl-xL, Bcl-2, MMP2 and MMP9. The chemical and structural details of C1 were deduced through FT-IR, GC–MS, ESI–MS, 1H and 13C NMR [both 1D and 2D] spectra.

Expression of an endogenous galactose-binding lectin correlates with neoplastic progression in the colon

Cancer ◽  
1995 ◽  
Vol 75 (12) ◽  
pp. 2818-2826 ◽  
Author(s):  
Harald L Schoeppner ◽  
Avraham Raz ◽  
Samuel B. Ho ◽  
Robert S. Bresalier
Keyword(s):  
Neoplastic Progression ◽  
Binding Lectin ◽  
Galactose Binding Lectin

Cytotoxicity and Glycan-Binding Profile of a d-Galactose-Binding Lectin from the Eggs of a Japanese Sea Hare (Aplysia kurodai)

2011 ◽  
Vol 30 (7) ◽  
pp. 509-519 ◽  
Author(s):  
Sarkar M. A. Kawsar ◽  
Ryo Matsumoto ◽  
Yuki Fujii ◽  
Haruki Matsuoka ◽  
Naoko Masuda ◽  
...  
Keyword(s):  
Binding Profile ◽  
Sea Hare ◽  
Binding Lectin ◽  
Galactose Binding Lectin

scholarly journals Mechanical Studies of the Third Dimension in Cancer: From 2D to 3D Model

2021 ◽  
Vol 22 (18) ◽  
pp. 10098
Author(s):  
Francesca Paradiso ◽  
Stefano Serpelloni ◽  
Lewis W. Francis ◽  
Francesca Taraballi
Keyword(s):  
Cell Fate ◽  
Cancer Cell ◽  
Cell Types ◽  
3D Models ◽  
Mechanical Tests ◽  
Research Approach ◽  
3D Scaffold ◽  
Multicellular Spheroids ◽  
Multiple Cell ◽  
Third Dimension

From the development of self-aggregating, scaffold-free multicellular spheroids to the inclusion of scaffold systems, 3D models have progressively increased in complexity to better mimic native tissues. The inclusion of a third dimension in cancer models allows researchers to zoom out from a significant but limited cancer cell research approach to a wider investigation of the tumor microenvironment. This model can include multiple cell types and many elements from the extracellular matrix (ECM), which provides mechanical support for the tissue, mediates cell-microenvironment interactions, and plays a key role in cancer cell invasion. Both biochemical and biophysical signals from the extracellular space strongly influence cell fate, the epigenetic landscape, and gene expression. Specifically, a detailed mechanistic understanding of tumor cell-ECM interactions, especially during cancer invasion, is lacking. In this review, we focus on the latest achievements in the study of ECM biomechanics and mechanosensing in cancer on 3D scaffold-based and scaffold-free models, focusing on each platform’s level of complexity, up-to-date mechanical tests performed, limitations, and potential for further improvements.

scholarly journals Graphene-Augmented Nanofiber Scaffolds Trigger Gene Expression Switching of Four Cancer Cell Types

2018 ◽  
Vol 4 (5) ◽  
pp. 1622-1629 ◽  
Author(s):  
Jekaterina Kazantseva ◽  
Roman Ivanov ◽  
Michael Gasik ◽  
Toomas Neuman ◽  
Irina Hussainova
Keyword(s):  
Gene Expression ◽  
Cancer Cell ◽  
Cell Types ◽  
Nanofiber Scaffolds
Sign in / Sign up

Export Citation Format

Share Document