High efficiency two-photon uncaging coupled by the correction of spontaneous hydrolysis

2018 ◽  
Vol 16 (11) ◽  
pp. 1958-1970 ◽  
Author(s):  
Dénes Pálfi ◽  
Balázs Chiovini ◽  
Gergely Szalay ◽  
Attila Kaszás ◽  
Gergely F. Turi ◽  
...  

DNI-Glu proved to be several times more efficient, than MNI-Glu and other analogues.

RSC Advances ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 2646-2649 ◽  
Author(s):  
Lamiaa M. A. Ali ◽  
Emna Mathlouthi ◽  
Maëlle Cahu ◽  
Saad Sene ◽  
Morgane Daurat ◽  
...  

Mn2+-doped Prussian blue nanoparticles loaded with doxorubicin present high efficiency for combined photothermal and chemotherapy of cancer cells with a synergic effect under two-photon irradiation.


2014 ◽  
Vol 5 (16) ◽  
pp. 4747-4755 ◽  
Author(s):  
Ming Jin ◽  
Hong Hong ◽  
Jianchao Xie ◽  
Jean-Pierre Malval ◽  
Arnaud Spangenberg ◽  
...  

D–π–A type π-conjugated photoacid generators through the para-to-meta substitution strategy show high efficiency in photoinitiated cationic polymerization reactions at 405 nm and 800 nm excitation.


2012 ◽  
Vol 10 (01) ◽  
pp. 1250008 ◽  
Author(s):  
ZHI-WEI SUN ◽  
RUI-GANG DU ◽  
DONG-YANG LONG

A one-way quantum secure direct communication protocol with quantum identification utilizing two-photon three-qubit linear cluster states is proposed. The protocol can be used to transmit a secret message and identify user's identification simultaneously. The transmission of information is instantaneous, i.e. the information can be decoded during the transmission and no final transmission of additional information is needed. We prove its robustness against attacks: Any attempt of an adversary to obtain information (and even a bit of information) necessarily induces some errors that the legitimate parties could notice, even in noisy environments. Moreover, this protocol achieves a high efficiency and source capacity since more qubits can be encoded on the same photon.


2021 ◽  
Vol 2086 (1) ◽  
pp. 012174
Author(s):  
E A Viktorov ◽  
A A Pastor ◽  
P Yu Serdobintsev ◽  
K Miculis ◽  
N N Bezuglov

Abstract We studied experimentally and theoretically the ionization of a two-photon excited superposition of 4f states of Xe atom by probe femtosecond pulse laser in a supersonic beam. The revealed oscillation structure in the observed photoionization signals is associated with the interference of the coherently populated components of Xe doublet states. Our results demonstrate the high efficiency of proposed experimental scheme for registering quantum beats in the photoelectron current.


2022 ◽  
Author(s):  
Yifan Wang ◽  
Yao Zheng ◽  
Yongxian Xu ◽  
Rongrong Li ◽  
Yameng Zheng ◽  
...  

Two-photon optogenetics enables selectively stimulating individual cells for manipulating neuronal ensembles. As the general photostimulation strategy, the patterned two-photon excitation has enabled millisecond-timescale activation for single or multiple neurons, but its activation efficiency is suffered from high laser power due to low beam-modulation efficiency. Here, we develop a high-efficiency beam-shaping method based on the Gerchberg-Saxton (GS) algorithm with spherical-distribution initial phase (GSSIP) to reduce the patterned two-photon excitation speckles and intensity. It can well control the phase of shaped beams to attain speckle-free accurate patterned illumination with an improvement of 44.21% in the modulation efficiency compared with that of the traditional GS algorithm. A combination of temporal focusing and the GSSIP algorithm (TF-GSSIP) achieves patterned focusing through 500-μm-thickness mouse brain slices, which is 2.5 times deeper than the penetration depth of TF-GS with the same signal-to-noise ratio (SNR). With our method, the laser power can be reduced to only 55.56% of that with traditional method (the temporal focusing with GS, TF-GS) to reliably evoke GCaMP6s response in C1V1-expressing cultured neurons with single-cell resolution. Besides, the photostimulation efficiency is remarkably increased by 80.19% at the same excitation density of 0.27 mW/μm2. This two-photon stimulation method with low-power, reliable and patterned illumination may pave the way for analyzing neural circuits and neural coding and decoding mechanism.


Author(s):  
Xiao Liu ◽  
Nan-Nan Zhang ◽  
Lei Guo ◽  
Zhong-Chao Wei ◽  
Yin-Yin Li ◽  
...  

MRS Bulletin ◽  
2005 ◽  
Vol 30 (12) ◽  
pp. 976-982 ◽  
Author(s):  
Da Yang ◽  
Shalin J. Jhaveri ◽  
Christopher K. Ober

AbstractThe controlled formation of submicrometer-scale structures in three dimensions is of increasing interest in many applications. Not intended to produce the smallest structures, but instead aimed at complex topographies, two-photon lithography is an intrinsic 3D lithography technique that enables the fabrication of structures difficult to access by conventional single-photon processes with far greater spatial resolution than other 3D microfabrication techniques. By tightly focusing a femtosecond laser beam into a resin, subsequent photo-induced reactions such as polymerization occur only in the close vicinity of the focal point, allowing the fabrication of a 3D structure by directly writing 3D patterns. The current research effort in two-photon lithography is largely devoted to the design and synthesis of high-efficiency photoinitiators and sensitizers, as well as the development of new materials and systems. This article provides an overview of the progress in two-photon processes for the formation of complex images and the development of patterned structures.


2000 ◽  
Vol 323 (1-2) ◽  
pp. 111-116 ◽  
Author(s):  
M Fakis ◽  
J Polyzos ◽  
G Tsigaridas ◽  
J Parthenios ◽  
A Fragos ◽  
...  

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