scholarly journals Quantitative determination of deoxyribonucleic acid in rat brain

1969 ◽  
Vol 115 (3) ◽  
pp. 563-568 ◽  
Author(s):  
N. W. Penn ◽  
R. Suwalski
Keyword(s):  
Rat Brain ◽  
Alkaline Hydrolysis ◽  
Present Method ◽  
Potassium Hydroxide ◽  
Deoxyribonucleic Acid ◽  
Maximum Yield ◽  
Spectrophotometric Analysis ◽  
Optimum Concentration ◽  
Hydrolysis Of

1. A procedure is given for spectrophotometric analysis of rat brain DNA after its resolution into component bases. Amounts of tissue in the range 50–100mg. can be used. 2. The amount of DNA obtained by the present method is 80% greater than that reported for rat brain by a previous procedure specific for DNA thymine. Identity of the material is established by the base ratios of purines and pyrimidines. The features responsible for the higher yield are the presence of dioxan during alkaline hydrolysis of tissue, the determination of the optimum concentration of potassium hydroxide in this step and omission of organic washes of the initial acid-precipitated residues. 3. The requirement for dioxan during alkaline hydrolysis suggests a possible association of brain DNA with lipid. The concentration of potassium hydroxide that gives maximum yield is 0·1m, indicating that there may be internucleotide linkages in this DNA that are more sensitive to alkali than those of liver or thymus DNA. 4. This procedure gives low yields of DNA from liver. It is not suitable for analysis of the DNA from this tissue.

Fluorometric determination of carnitine in serum with immobilized carnitine dehydrogenase and diaphorase

1990 ◽  
Vol 36 (12) ◽  
pp. 2072-2076 ◽  
Author(s):  
K Matsumoto ◽  
Y Yamada ◽  
M Takahashi ◽  
T Todoroki ◽  
K Mizoguchi ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Present Method ◽  
Potassium Hydroxide ◽  
Immobilized Enzymes ◽  
Fluorometric Determination ◽  
Serum Samples ◽  
Clinical Laboratories ◽  
Analytical Recovery ◽  
Hydrolysis Of

Abstract A fluorometric flow-injection method for determining carnitine with use of immobilized enzymes carnitine dehydrogenase (EC 1.1.1.108) and diaphorase (EC 1.8.1.4) was developed and applied to the assay of carnitine in serum of patients treated with valproic acid. After fractionation and hydrolysis of carnitines in serum samples by perchloric acid and potassium hydroxide, liberated carnitine was converted to resorufin by immobilized carnitine dehydrogenase and diaphorase in the presence of beta-NAD+ (1.0 mmol/L), resazurin (12.5 mumol/L), and Tris acetate (0.6 mol/L, pH 9.0) at 37 degrees C. The fluorescence intensity of resorufin was monitored at lambda Ex 560 nm and lambda Em 580 nm. The calibration curve was linear for carnitine amounts from 0.1 to 1.0 nmol. Quantitative analytical recovery and satisfactory within- and between-run imprecision of carnitine in each carnitine fraction were obtained. Interference by bilirubin, serum albumin, and hemoglobin was negligible. Carnitine deficiencies were detected in about 20% of the valproic acid-treated patients (n = 198). The present method should be useful for monitoring carnitine deficiencies in clinical laboratories.

Spectrophotometric Determination of Carbaryl in Grains

1982 ◽  
Vol 65 (1) ◽  
pp. 32-34
Author(s):  
Kalapanda M Appaiah ◽  
Rasamsetti Ramakrishna ◽  
Kadari R Subbarao ◽  
Omprakash Kapur
Keyword(s):  
Spectrophotometric Determination ◽  
Absorption Maximum ◽  
Potassium Hydroxide ◽  
Oxidizing Agent ◽  
Spectrophotometric Measurement ◽  
The Relationship ◽  
Hydrolysis Of

Abstract A method has been developed for determining carbaryl (1-naphthyl N-methyl carbamate) in grains, based on hydrolysis of carbaryl with methanolic potassium hydroxide to 1-naphthol, reaction with 4-aminophenazone in the presence of alkaline oxidizing agent, and spectrophotometric measurement at the absorption maximum at 475 nm. The relationship between absorbance and concentration is linear in the range of 0.5-20 μg/mL. The method can be applied to levels as low as 0.3 ppm carbaryl in grains.

Determination of VC-13 Nemacide Residues on Crops

1964 ◽  
Vol 47 (2) ◽  
pp. 287-288
Author(s):  
John I Teasley
Keyword(s):  
Alkaline Hydrolysis ◽  
Collaborative Study ◽  
Potassium Ferricyanide ◽  
Hydrolysis Of

Abstract A collaborative study was made of a method for the determination of VC-13 Nemacide residues, based on the alkaline hydrolysis of the pesticide to produce 2,4-dichlorophenol, which is measured colorimetrically with 4-aminoantipyrine and potassium ferricyanide. Results indicate that cleanup technique is critical and not effective for some crops. Several changes in the method were recommended for the next study. Three of five collaborators obtained satisfactory recoveries at levels of 50—250 ppb.

scholarly journals HIDROLISIS RUMPUT LAUT (Glacilaria sp.) MENGGUNAKAN KATALIS ENZIM DAN ASAM UNTUK PEMBUATAN BIOETANOL

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Yohanes Armawan Sandi ◽  
Wiwik Susanah Rita ◽  
Yenni Ciawi
Keyword(s):  
Gas Chromatography ◽  
Hydrochloric Acid ◽  
Sulphuric Acid ◽  
Ethanol Concentration ◽  
Reducing Sugar ◽  
Optimum Concentration ◽  
Raw Material ◽  
Optimum Length ◽  
Hydrolysis Of

The aim of this research is to determine the effect of enzyme and acids concentration on the yield of glucose produced in the hydrolysis of Glacilaria sp. in the production of bioethanol. The concentrations of cellulase used were 200 units/mL, 400 units/mL, 600 units/mL, 800 units/mL and the concentration of sulphuric acid (H2SO4) and hydrochloric acid (HCl) used were 1%, 3%, 5%, 7% (w/v). The concentration of reduction sugar was determined using Anthrone and analyzed using UV-Vis spectrophotometry and the determination of ethanol concentration was carried out by using gas chromatography. The results showed that the contents of reducing sugar produced by sulphuric acid (H2SO4) hydrolysis were 26,19%; 36,69%; 41,40%; 45,0% (v/v), by hydrochloric acid (HCl) were 12,12%; 14,03%; 15,17%; 16,50% (v/v), and by cellulase enzyme were 46,15%; 46,73%; 47,68%; 48,25% (v/v). Optimum concentration of reducing sugar produced by hydrolysis using 800 units/mL cellulase was 48,25% (v/v). The optimum length of fermentation to produce bioethanol using Glacilaria sp. as raw material was 5 days. In the fermentation, inoculum with a concentrations of 5% and 10% (w/v) produced 0,85% and 1,51% (v/v) ethanol.

scholarly journals Extractive Spectrophotometric Determination of Malathion in Environmental Samples Using Gention Violet

2005 ◽  
Vol 2 (3) ◽  
pp. 187-192 ◽  
Author(s):  
K. M. Reddy ◽  
K. Suvardhan ◽  
K. Suresh Kumar ◽  
D. Rekha ◽  
P. Chiranjeevi
Keyword(s):  
Spectrophotometric Method ◽  
Alkaline Hydrolysis ◽  
Environmental Samples ◽  
Sodium Ethoxide ◽  
Ion Pair ◽  
Soil Samples ◽  
Organic Layer ◽  
Cationic Dye ◽  
Hydrolysis Of

A new simple and selective spectrophotometric method is developed for the determination of malathion by using Gention violet is described. The method was based on the alkaline hydrolysis of malathion in presence of sodium ethoxide to form sodium dimethyl dithiophosphate (Na-DMDTP). The Na-DMDTP was formed as an ion-pair complex with cationic dye, gention violet. The ion-pair complex was extracted into chloroform. The color of the organic layer was measured at 587 nm. The method was applied to the determination of malathion residues in water, grain and soil samples

scholarly journals Determination of the Bond Ruptured in the Alkaline Hydrolysis of Vinyl Acetate.

1972 ◽  
Vol 26 ◽  
pp. 855-856 ◽  
Author(s):  
Erkki K. Euranto ◽  
Antero Alhoniemi ◽  
A. Kjekshus ◽  
A. F. Andresen ◽  
W. B. Pearson ◽  
...  
Keyword(s):  
Vinyl Acetate ◽  
Alkaline Hydrolysis ◽  
Hydrolysis Of

Colorimetric Determination of Propoxur and Its Residues in Vegetables

1983 ◽  
Vol 66 (1) ◽  
pp. 105-107 ◽  
Author(s):  
Kalapanda M Appaiah ◽  
Omprakash Kapur ◽  
Krishnarajpet V Nagaraja
Keyword(s):  
Absorption Maximum ◽  
Potassium Hydroxide ◽  
Colorimetric Determination ◽  
Diaminodiphenyl Sulfone ◽  
Beer's Law ◽  
Beer’S Law ◽  
Hydrolysis Of

Abstract A method has been developed for the determination of propoxur (o-isopropoxyphenyl N-methylcarbamate) based on the hydrolysis of propoxur with methanolic potassium hydroxide to its phenol and coupling with diazotized 4,4-diaminodiphenyl sulfone. The orange complex formed has an absorption maximum at 500 nm and obeys Beer’s law in the range 0.25-5.0 μg/mL. The method can be applied to levels as low as 0.5 ppm propoxur from vegetables.

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