scholarly journals Bioconjugation and stabilisation of biomolecules in biosensors

2016 ◽  
Vol 60 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Susana Liébana ◽  
Guido A. Drago
Keyword(s):  
Protein Stability ◽  
Functional Groups ◽  
Magnetic Particles ◽  
Stability Studies ◽  
Shelf Stability ◽  
Protein Polymer ◽  
Protein Immobilisation ◽  
Covalent Immobilisation ◽  
Polymer Interactions ◽  
The Stability

Suitable bioconjugation strategies and stabilisation of biomolecules on electrodes is essential for the development of novel and commercially viable biosensors. In the present review, the functional groups that comprise the selectable targets for practical bioconjugation methods are discussed. We focus on describing the most common immobilisation techniques used in biosensor construction, which are classified into irreversible and reversible methods. Concerning the stability of proteins, the two main types of stability may be defined as (i) storage or shelf stability, and (ii) operational stability. Both types of stability are explained, as well as the introduction of an electrophoretic technique for predicting protein–polymer interactions. In addition, solution and dry stabilisation as well as stabilisation using the covalent immobilisation of proteins are discussed including possible factors that influence stability. Finally, the integration of nanomaterials, such as magnetic particles, with protein immobilisation is discussed in relation to protein stability studies.

Applicability of Instability Index for In vitro Protein Stability Prediction

2019 ◽  
Vol 26 (5) ◽  
pp. 339-347 ◽  
Author(s):  
Dilani G. Gamage ◽  
Ajith Gunaratne ◽  
Gopal R. Periyannan ◽  
Timothy G. Russell
Keyword(s):  
Protein Stability ◽  
Caulobacter Crescentus ◽  
Effect Of Temperature ◽  
Instability Index ◽  
Dipeptide Composition ◽  
Experimental Conditions ◽  
The Stability ◽  
Vitro Protein

Background: The dipeptide composition-based Instability Index (II) is one of the protein primary structure-dependent methods available for in vivo protein stability predictions. As per this method, proteins with II value below 40 are stable proteins. Intracellular protein stability principles guided the original development of the II method. However, the use of the II method for in vitro protein stability predictions raises questions about the validity of applying the II method under experimental conditions that are different from the in vivo setting. Objective: The aim of this study is to experimentally test the validity of the use of II as an in vitro protein stability predictor. Methods: A representative protein CCM (CCM - Caulobacter crescentus metalloprotein) that rapidly degrades under in vitro conditions was used to probe the dipeptide sequence-dependent degradation properties of CCM by generating CCM mutants to represent stable and unstable II values. A comparative degradation analysis was carried out under in vitro conditions using wildtype CCM, CCM mutants and two other candidate proteins: metallo-β-lactamase L1 and α -S1- casein representing stable, borderline stable/unstable, and unstable proteins as per the II predictions. The effect of temperature and a protein stabilizing agent on CCM degradation was also tested. Results: Data support the dipeptide composition-dependent protein stability/instability in wt-CCM and mutants as predicted by the II method under in vitro conditions. However, the II failed to accurately represent the stability of other tested proteins. Data indicate the influence of protein environmental factors on the autoproteolysis of proteins. Conclusion: Broader application of the II method for the prediction of protein stability under in vitro conditions is questionable as the stability of the protein may be dependent not only on the intrinsic nature of the protein but also on the conditions of the protein milieu.

scholarly journals Functional Groups Required for the Stability of Yeast RNA Triphosphatasein Vitroandin Vivo

2001 ◽  
Vol 276 (32) ◽  
pp. 30514-30520 ◽  
Author(s):  
Martin Bisaillon ◽  
Stewart Shuman
Keyword(s):  
Functional Groups ◽  
The Stability

scholarly journals Stability Studies of Transition Metal Chelates of 5-Bromosalicylidene-4-methoxyaniline andSalicylidene-2,3-dimethylaniline as Ligands

2011 ◽  
Vol 8 (4) ◽  
pp. 1911-1915
Author(s):  
N. G. Nadkarni ◽  
K. V. Mangaonkar
Keyword(s):  
Ionic Strength ◽  
Transition Metal ◽  
Stability Constants ◽  
Metal Chelates ◽  
Ternary Complexes ◽  
Water Medium ◽  
Stability Studies ◽  
Binary And Ternary Complexes ◽  
Transition Metal Chelates ◽  
The Stability

Binary and ternary complexes of the type M-Y and M-X-Y [M = Mn(II), Ni(II), Cu(II) and Zn(II); X = 5-bromosalicylidene-4-methoxyaniline and Y = salicylidene-2,3-dimethylaniline] have been examined pH-metrically at 27±0.5°C and at constant ionic strength, μ = 0.1 M (KCl) in 75 : 25(v/v) 1,4-dioxne-water medium. The stability constants for binary (M-Y) and ternary (M-X-Y) systems were calculated.

DISPERSION OF COBALT FERRITE FUNCTIONALIZED GRAPHENE NANOPLATELETS IN PLA FOR EMI SHIELDING APPLICATIONS

2021 ◽  
Author(s):  
KANAT ANURAKPARADORN ◽  
ALAN TAUB ◽  
ERIC MICHIELSSEN
Keyword(s):  
Functional Groups ◽  
Electromagnetic Interference ◽  
High Speed ◽  
Magnetic Particles ◽  
Cobalt Ferrite ◽  
Reduction Process ◽  
Cost Effective ◽  
Graphene Nanoplatelets ◽  
Angle Of Incidence ◽  
Emi Shielding

The proliferation of wireless technology calls for the development of cost-effective Electromagnetic Interference (EMI) shielding materials that reduce the susceptibility of high-speed electronic circuits to undesired incoming radiation. Ideally, such materials offer protection over wide frequency ranges and are insensitive to the polarization or angle of incidence of the impinging fields. Here, next-generation EMI shielding materials composed of polymer composites with conductive and magnetic fillers are introduced. It is shown that careful control of the concentration and dispersion of the polymers’ conductive and magnetic constituents permits tuning of the composites’ intrinsic electrical and magnetic properties. The resulting EMI shields are lightweight, cheap and offer greater protection than traditional metal gaskets and foams. In this work, cobalt ferrite magnetic nanoparticles (CoFe2O4) decorated on graphene-based material were dispersed in polylactic acid (PLA) matrix for high EM absorption level in X-band (8-12 GHz). The decoration of the magnetic particles was performed on the as-prepared conductive graphene nanoplatelets (GNP) and reduced graphene oxide (rGO). GNP composites exhibited higher DC conductivity, and permittivity than rGO composites. This is attributed to issues associated with the reduction process, including a lack of conductivity due to the insulated oxygen functional groups and the reduction in the lateral size. Compared with rGOs, the lack of out-plane functional groups causes the cobalt ferrite nanoparticles to agglomerate and not cover the entire surface of the GNPs. These morphological differences improve the magnetization and EM absorption of the composite system. The compatibilizer (pyrene-PLA-OH) was added to the composites to enhance dispersion of the GNPs in the polymer matrix which benefits in higher absorption of the shield. The influence of the compatibilizer on parameter, the reflection loss (RL) of the composite were determined from the characterized intrinsic properties

Magnetorheological fluid based on amorphous Fe-Si-B alloy magnetic particles

2021 ◽  
pp. 1045389X2110643
Author(s):  
Chuncheng Yang ◽  
Zhong Liu ◽  
Xiangyu Pei ◽  
Cuiling Jin ◽  
Mengchun Yu ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Field Strength ◽  
Rheological Property ◽  
Magnetic Particles ◽  
Annealing Treatment ◽  
Magnetorheological Fluid ◽  
The Stability ◽  
The Magnetic Field ◽  
Amorphous Particle ◽  
Better Than

Magnetorheological fluids (MRFs) based on amorphous Fe-Si-B alloy magnetic particles were prepared. The influence of annealing treatment on stability and rheological property of MRFs was investigated. The saturation magnetization ( Ms) of amorphous Fe-Si-B particles after annealing at 550°C is 131.5 emu/g, which is higher than that of amorphous Fe-Si-B particles without annealing. Moreover, the stability of MRF with annealed amorphous Fe-Si-B particles is better than that of MRF without annealed amorphous Fe-Si-B particles. Stearic acid at 3 wt% was added to the MRF2 to enhance the fluid stability to greater than 90%. In addition, the rheological properties demonstrate that the prepared amorphous particle MRF shows relatively strong magnetic responsiveness, especially when the magnetic field strength reaches 365 kA/m. As the magnetic field intensified, the yield stress increased dramatically and followed the Herschel-Bulkley model.

scholarly journals A glycine-specific N-degron pathway mediates the quality control of protein N-myristoylation

Science ◽  
2019 ◽  
Vol 365 (6448) ◽  
pp. eaaw4912 ◽  
Author(s):  
Richard T. Timms ◽  
Zhiqian Zhang ◽  
David Y. Rhee ◽  
J. Wade Harper ◽  
Itay Koren ◽  
...  
Keyword(s):  
Quality Control ◽  
Protein Stability ◽  
Protein Degradation ◽  
E3 Ligase ◽  
Cleavage Sites ◽  
Caspase Cleavage ◽  
E3 Ligases ◽  
Ring E3 Ligase ◽  
The Stability ◽  
Ring E3

The N-terminal residue influences protein stability through N-degron pathways. We used stability profiling of the human N-terminome to uncover multiple additional features of N-degron pathways. In addition to uncovering extended specificities of UBR E3 ligases, we characterized two related Cullin-RING E3 ligase complexes, Cul2ZYG11B and Cul2ZER1, that act redundantly to target N-terminal glycine. N-terminal glycine degrons are depleted at native N-termini but strongly enriched at caspase cleavage sites, suggesting roles for the substrate adaptors ZYG11B and ZER1 in protein degradation during apoptosis. Furthermore, ZYG11B and ZER1 were found to participate in the quality control of N-myristoylated proteins, in which N-terminal glycine degrons are conditionally exposed after a failure of N-myristoylation. Thus, an additional N-degron pathway specific for glycine regulates the stability of metazoan proteomes.

scholarly journals The atomic process in ferromagnetic Induction

1922 ◽  
Vol 100 (706) ◽  
pp. 449-460 ◽  
Keyword(s):  
Magnetic Particles ◽  
Strong Field ◽  
Molecular Magnets ◽  
Magnetic Element ◽  
Atomic Process ◽  
Dissipation Of Energy ◽  
Unstable Phase ◽  
Complete Alignment ◽  
The Stability ◽  
Ferromagnetic Substance

The object of this paper is to amend, in an important particular, the theory of ferromagnetic induction put forward by me more than 30 years ago, and to describe a new model. That theory was itself a modification of the earlier theory of Weber. To Weber is due the fundamental notion that a substance contains minute particles, each of which acts as a magnet, and that in the process of magnetising a ferromagnetic substance these are turned into more or less complete alignment. The ultimate magnetic particles use to be called “molecular magnets”: we now recognise them as attributes of the atom, not of the molecule, and (in all probability) they derive their magnetic moment from the circulation of electricity in electron orbits or in ring electrons. What turns is not the molecule nor the atom, but something within the atom. The characteristics which distinguish ferromagnetic substances from other paramagnetics are: (1) the much larger amount of magnetism they can acquire under the action of an impressed field; (2) the fact that the acquired magnetism tends towards a saturation limit when the field is progressively increased; (3) the fact that the acquired magnetism shows hysteresis with respect to variations of the field, except in certain small initial changes. Weber’s theory explained (1) and (2). My modification of it explained, in addition, (3) as an effect of the irreversible action which occurs when the equilibrium of a magnetic element becomes unstable through change in the externally impressed magnetic force, and it swings over, with dissipation of energy, into a new position of stability. The stability in both positions is sufficiently explained by magnetic forces only. In breaking away from one stable position it is deflected at first in a quasi­-elastic (reversible) manner until the external force reaches a certain value at which the equilibrium is upset. The essence of hysteresis is the turning from one position of stability to another, through a region of instability. If the conditions are such that there is no unstable phase in the turning, then there is no dissipation of energy, and consequently no hysteresis. This occurs in very feeble magnetisation, when the deflections are reversible; it also occurs if the piece be caused to rotate in a field of great strength. J. Swinburne pointed out that, as a consequence of my theory, hysteresis should vanish when a cylinder of ferromagnetic metal is rotated in a very strong field, and this curious result was confirmed experimentally by F. G. Baily.

Stability of extemporaneously prepared rosuvastatin oral suspension

2017 ◽  
Vol 74 (19) ◽  
pp. 1579-1583 ◽  
Author(s):  
Abdel Naser Zaid ◽  
Rania Shtayah ◽  
Ayman Qadumi ◽  
Mashour Ghanem ◽  
Rawan Qedan ◽  
...  
Keyword(s):  
Relative Humidity ◽  
High Performance ◽  
Room Temperature ◽  
Microbial Contamination ◽  
Active Pharmaceutical Ingredient ◽  
Storage Conditions ◽  
Dissolution Profile ◽  
Stability Studies ◽  
Organoleptic Properties ◽  
The Stability

Abstract Purpose The stability of an extemporaneously prepared rosuvastatin suspension stored over 30 days under various storage conditions was evaluated. Methods Rosuvastatin suspension was extemporaneously prepared using commercial rosuvastatin tablets as the source of active pharmaceutical ingredient. The organoleptic properties, dissolution profile, and stability of the formulation were investigated. For the stability studies, samples of the suspension were stored under 2 storage conditions, room temperature (25 °C and 60% relative humidity) and accelerated stability chambers (40 °C and 75% relative humidity). Viscosity, pH, organoleptic properties, and microbial contamination were evaluated according to the approved specifications. High-performance liquid chromatography was used for the analysis and quantification of rosuvastatin in selected samples. Microbiological investigations were also conducted. Results The prepared suspension showed acceptable organoleptic properties. It showed complete release of rosuvastatin within 15 minutes. The pH of the suspension was 9.8, which remained unchanged during the stability studies. The microbiological investigations demonstrated that the preparation was free of any microbial contamination. In addition, the suspension showed stability within at least the period of use of a 100-mL rosuvastatin bottle. Conclusion Extemporaneously prepared rosuvastatin 20-mg/mL suspension was stable for 30 days when stored at room temperature.

scholarly journals FORMULATION, CHARACTERIZATION AND STABILITY STUDY OF FAST DISSOLVING THIN FILM CONTAINING ASTAXANTHIN NANOEMULSION USING HYDROXYPROPYLMETHYL CELLULOSE POLYMER

2021 ◽  
pp. 17-22
Author(s):  
LUSI NURDIANTI ◽  
IYAN SOPYAN ◽  
TAOFIK RUSDIANA
Keyword(s):  
Thin Film ◽  
Mechanical Characteristics ◽  
Storage Conditions ◽  
Stability Study ◽  
Matrix System ◽  
Stability Studies ◽  
Casting Method ◽  
Hydroxypropylmethyl Cellulose ◽  
The Stability ◽  
Physical And Mechanical Characteristics

Objective: The present study was conducted to formulate and characterize the thin film containing astaxanthin nanoemulsion (TF-ASN) using Hydroxypropylmethyl Cellulose (HPMC) polymer as a film matrix system. The stability studies in different storage conditions were also performed. Methods: Astaxanthin nanoemulsion (As-NE) was prepared by using self-nanoemulsifying method, followed by incorporation into the HPMC matrix system by solvent casting method to forming TF-ASN. Evaluation of TF-ASN was performed by physical and mechanical characterizations. Stability study was carried out in both of accelerated (temperature of 40±2 °C/75±5% RH) and non-accelerated (at ambient temperature) conditions. Assay of astaxanthin in individual TF-ASN was determined compared to pure astaxanthin. Results: TF-ASN had good physical and mechanical characteristics that suitable for intraoral administration. Conclusion: For the study of stability under different storage conditions, it was proven that nanoemulsion form was packed in a HPMC matrix could enhance the stability of the astaxanthin.

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