Zytotoxische, antiproliferative und antiangiogenetische Wirkung von Helleborus niger auf Endothelzellen und verschiedene Tumorzelllinien in vitro

2016 ◽  
Vol 37 (S 01) ◽  
Author(s):  
J Felenda ◽  
C Beckmann ◽  
FC Stintzing
Keyword(s):  
Helleborus Niger

scholarly journals Effect of different sucrose and nitrogen salt levels in the medium and temperature on in vitro propagation of Helleborus niger L.

2015 ◽  
Vol 32 (2) ◽  
pp. 161-171 ◽  
Author(s):  
Eleonora Anna Gabryszewska
Keyword(s):  
Developmental Stages ◽  
Leaf Shape ◽  
Morphological Observation ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Ex Vitro ◽  
Axillary Shoots ◽  
The Media ◽  
Helleborus Niger

<em>Helleborus niger</em> L. is a rhizomatous, herbaceous perennial with overwintering, divided, basal leaves. The objective of the study was to investigate the influence of different levels of sucrose (10, 20, 30, 40, 50, 60, 70, and 80 g l<sup>−1</sup>) and nitrogen salts (25%, 50%, and 100% according to MS medium) as well as temperature (15°C, 20°C) on in vitro multiplication and rooting and ex vitro acclimatization of <em>H. niger</em>. The growth and multiplication of axillary shoots were performed on modified MS medium supplemented with various growth regulators (2iP, BAP and kinetin – each at a concentration of 1.0 mg l<sup>−1</sup>, GA<span><sub>3</sub></span> 2.5 mg l<sup>−1</sup>). For the induction of roots, the medium was supplemented with IBA 1 mg l<sup>−1</sup> and NAA 0.1 mg l<sup>−1</sup>. Rooted plants were transplanted in a peat–perlite substrate (4:1) in a heated greenhouse for ex vitro acclimatization. The multiplication rate of <em>H. niger</em> shoots, in vitro rooting, and ex vitro acclimatization were strongly dependent on the sucrose/nitrogen salt relationship in the medium. The highest multiplication rate of axillary shoots (3.7) was found at a temperature of 15°C or 20°C, on the medium with cytokinins and GA<span><sub>3</sub></span> supplemented with sucrose 20–30 g l<sup>−1</sup> and nitrogen salts at 50%. Sucrose at a concentration of 50 g l<sup>−1</sup> strongly stimulated the number of roots per microplant (5.8–6.0) on the media with a reduced level of nitrogen salts (25% and 50%) when the temperatures were 20°C and 15°C, respectively. The plants rooted on the media with a high sucrose/nitrogen salt ratio showed acclimatization rates which ranged from 82% to 100%. Morphological observation of plantlets revealed obvious differences in leaf shape and size and the architecture of the root system as well as differences in the developmental stages of shoots grown on media with different sucrose and nitrogen salt concentrations.

scholarly journals Helleborus Niger: Systematics, Ecology, Pollination and Production Technology

2021 ◽  
Author(s):  
Andrej Šušek
Keyword(s):  
Production Technology ◽  
Ornamental Plants ◽  
Plant Ecology ◽  
Potted Plants ◽  
The Future ◽  
Genetically Improved ◽  
Helleborus Niger ◽  
Christmas Rose

The scientific monograph covers the botanical classification of the genus Helleborus L. and the Christmas rose species (Helleborus niger L.), which has undergone numerous changes throughout its botanical history. The Christmas rose is becoming increasingly important in the ornamental plant market. Its characteristic flowering in the coldest months of the year represents a great advantage over many other species of ornamental plants. As the Christmas rose does not require high temperatures to start flowering, its cultivation can be considered affordable and environmentally friendly. The supply of attractive varieties is likely to be one of the key factors that will affect its popularity in the future. The number of genetically improved (bred) varieties is limited on the market, so selective breeding of Christmas rose will become inevitable in the future. The presented results of pollination analyses, related to insect activity, are important for breeding. Observations indicate that the Christmas rose is an entomophilous and a predominantly allogamous species. Knowledge of plant ecology and cultivation technology will also be exceptionally important. In intensive cultivation, the production technology for Christmas roses is still to a large extent incomplete. The monograph includes scientific findings on the ecology and biology of the Christmas rose, its reproductive processes, and the production technology for potted plants. The monograph also presents the results of the study on the effects of beneficial microorganisms (bacteria and endomycorrhizal fungi) on the growth and development of seedlings from in vivo and in vitro environments. The results of the study indicate that biotisation is a useful technology and that inoculation with specific microorganisms has a beneficial effect on growth.

In vitro cloning of Helleborus niger

2002 ◽  
Vol 20 (10) ◽  
pp. 895-900 ◽  
Author(s):  
M. Seyring
Keyword(s):  
Helleborus Niger

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

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