Reaction Time and Time Course of Neuronal Responses in MT and MST at Different Stimulus Contrasts

Perception ◽  
1996 ◽  
Vol 25 (1_suppl) ◽  
pp. 157-157 ◽  
Author(s):  
A Thiele ◽  
K-P Hoffmann
Keyword(s):  
Reaction Time ◽  
Single Cell ◽  
Single Cells ◽  
Direction Selectivity ◽  
Contrast Level ◽  
Population Activity ◽  
Temporal Area ◽  
Population Mean ◽  
The Mean ◽  
Mean Reaction Time

Direction-selective neurons from the middle temporal area (MT) and the middle superior temporal area (MST) were recorded while a monkey performed a direction discrimination task. Stimuli consisted of evenly spaced bars moving in one of the four cardinal directions. Monkey's reaction time, single-cell latency, and direction selectivity were calculated when stimuli of 53%, 24%, and 4% contrast were presented, and the monkey indicated a correct decision. Mean reaction time was 359±77 ms at 53% contrast, 391±107 ms at 24% contrast, and 582±374 ms at 4% contrast. Most neurons exhibiting direction selective responses at 53% contrast was also active at 24% contrast (MT, 99%; MST, 88%). The number of neurons still exhibiting stimulus-related activity at 4% contrast dramatically decreased (MT to 28%; MST to 41%). Shortest latencies were found at high contrast level (53% contrast; MT, 29 ms; population mean, 76±40 ms; MST, 35 ms; population mean, 77±27 ms). Single cell and population latency increased at lower contrast (4% contrast: MT minimum, 86 ms; population mean, 180±76 ms; MST minimum, 97 ms; population mean, 205±56 ms). This indicates that the mean increase in latency at the single-cell level only partially reflects the increase in reaction time (mean reaction time increased by 223 ms, while mean single-cell latency increased by ∼100 ms in MT and MST). We therefore calculated the normalised population response at different contrast levels. The maximal population activity was always found at the highest contrast level and this was set to 1. In MT it took 75 – 80 ms from stimulus onset until half maximal activity (0.5) was reached at 53% contrast. To reach 0.5 took 85 – 90 ms at 24% contrast and 205 – 210 ms at 4% contrast. For MST the respective values were 85 ms (53% contrast), 90 ms (24% contrast) and 255 ms (4%) contrast. Thus the time to reach half the maximal population activity much better reflects the reaction time than the mean of the latencies calculated from single cells.

Effect of Visual Complexity in Identification of Tachistoscopic Images

1994 ◽  
Vol 78 (3) ◽  
pp. 971-978 ◽  
Author(s):  
Robert Geheb ◽  
Keith E. Whitfield ◽  
Linda Brannon
Keyword(s):  
Reaction Time ◽  
Left Hemisphere ◽  
Right Hemisphere ◽  
Visual Complexity ◽  
Reaction Times ◽  
Right Cerebral Hemisphere ◽  
The Mean ◽  
The Times ◽  
The Right ◽  
Mean Reaction Time

The present study of gender differences in hemispheric processing involved identification of tachistoscopically presented images of varying complexity. A computerized tachistoscopic program was administered to 24 men and 34 women. Time to identify contour and detailed pictures presented to the left or right cerebral hemisphere was recorded. Mean reaction time for contour pictures was significantly faster than for detailed pictures, and mean reaction time to the right hemisphere was significantly faster than that to the left hemisphere. The mean reaction time for men to identify pictures exposed to the left hemisphere was significantly slower than that for exposure to the right hemisphere for women. The mean reaction time for both men and women to identify contour pictures exposed to the right hemisphere was significantly faster than the mean time to identify detailed pictures presented to the left hemisphere. The interaction of gender, hemisphere, and complexity was also significant in that mean reaction times for men to identify detailed pictures presented to the left hemisphere were slower than the times for women to identify contour pictures presented to the right hemisphere. The results are discussed in relation to theories about hemispheres, gender, and differences in picture features.

scholarly journals Noise-driven growth rate gain in clonal cellular populations

2016 ◽  
Vol 113 (12) ◽  
pp. 3251-3256 ◽  
Author(s):  
Mikihiro Hashimoto ◽  
Takashi Nozoe ◽  
Hidenori Nakaoka ◽  
Reiko Okura ◽  
Sayo Akiyoshi ◽  
...  
Keyword(s):  
Growth Rate ◽  
Population Growth ◽  
Single Cell ◽  
Single Cells ◽  
Population Level ◽  
Growth Conditions ◽  
Cell Dynamics ◽  
Generation Times ◽  
Complex Population ◽  
The Mean

Cellular populations in both nature and the laboratory are composed of phenotypically heterogeneous individuals that compete with each other resulting in complex population dynamics. Predicting population growth characteristics based on knowledge of heterogeneous single-cell dynamics remains challenging. By observing groups of cells for hundreds of generations at single-cell resolution, we reveal that growth noise causes clonal populations of Escherichia coli to double faster than the mean doubling time of their constituent single cells across a broad set of balanced-growth conditions. We show that the population-level growth rate gain as well as age structures of populations and of cell lineages in competition are predictable. Furthermore, we theoretically reveal that the growth rate gain can be linked with the relative entropy of lineage generation time distributions. Unexpectedly, we find an empirical linear relation between the means and the variances of generation times across conditions, which provides a general constraint on maximal growth rates. Together, these results demonstrate a fundamental benefit of noise for population growth, and identify a growth law that sets a “speed limit” for proliferation.

scholarly journals Imaging of respiratory-related population activity with single-cell resolution

2007 ◽  
Vol 292 (1) ◽  
pp. C508-C516 ◽  
Author(s):  
Frank Funke ◽  
Mathias Dutschmann ◽  
Michael Müller
Keyword(s):  
Single Cell ◽  
Neuronal Activity ◽  
Single Cells ◽  
Activity Patterns ◽  
Respiratory Rhythm ◽  
Network Activity ◽  
Respiratory Neurons ◽  
Ventrolateral Medulla ◽  
Population Activity ◽  
Respiratory Network

The pre-Bötzinger complex (PBC) in the rostral ventrolateral medulla contains a kernel involved in respiratory rhythm generation. So far, its respiratory activity has been analyzed predominantly by electrophysiological approaches. Recent advances in fluorescence imaging now allow for the visualization of neuronal population activity in rhythmogenic networks. In the respiratory network, voltage-sensitive dyes have been used mainly, so far, but their low sensitivity prevents an analysis of activity patterns of single neurons during rhythmogenesis. We now have succeeded in using more sensitive Ca2+ imaging to study respiratory neurons in rhythmically active brain stem slices of neonatal rats. For the visualization of neuronal activity, fluo-3 was suited best in terms of neuronal specificity, minimized background fluorescence, and response magnitude. The tissue penetration of fluo-3 was improved by hyperosmolar treatment (100 mM mannitol) during dye loading. Rhythmic population activity was imaged with single-cell resolution using a sensitive charge-coupled device camera and a ×20 objective, and it was correlated with extracellularly recorded mass activity of the contralateral PBC. Correlated optical neuronal activity was obvious online in 29% of slices. Rhythmic neurons located deeper became detectable during offline image processing. Based on their activity patterns, 74% of rhythmic neurons were classified as inspiratory and 26% as expiratory neurons. Our approach is well suited to visualize and correlate the activity of several single cells with respiratory network activity. We demonstrate that neuronal synchronization and possibly even network configurations can be analyzed in a noninvasive approach with single-cell resolution and at frame rates currently not reached by most scanning-based imaging techniques.

scholarly journals Oral Associative Reactions of Russians and Germans to Names of Mental States: Comparative and Quantitative Analysis

2020 ◽  
Vol 2020 (3) ◽  
pp. 46-57
Author(s):  
Ekaterina M. Alekseeva
Keyword(s):  
Quantitative Analysis ◽  
Reaction Time ◽  
Mental States ◽  
Cross Cultural ◽  
Experimental Procedure ◽  
Cross Cultural Study ◽  
Quantitative Content ◽  
The Mean ◽  
Speech Response ◽  
Mean Reaction Time

The article describes a cross-cultural study of associative oral representation of mental states. 32 Russian and 33German students – 53 female and 12 male aged from 20 up to 24 years participated in the research. The experimental procedure was developed on the basis of the DMDX program allowed to measure the time of speech response to the shown stimuli – names of 25 mental states. In the conditions of time deficiency probands had to call free and estimated associations (adjectives). The mean reaction time was calculated, quantitative content of associative fields was analyzed. In the Russian group associations (both free and estimated in the form of an adjective) to names of mental states were more quickly named. The average time of free oral associative reaction in the Russian group was 2114.6 ms, in the German - 2163.6 ms. The estimated associative response proved slower than the free associative response in both groups: the average time of the estimated oral associative reaction in the Russian group was 2246.9 ms, in the German – 2384.6 ms. During the experiment more evaluation associations in the form of adjectives were named than free ones. The vast majority of free associations to names of mental states were nouns. Most of the associations to names of mental states are unique.

scholarly journals Universal kinetics of imperfect reactions in confinement

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Thomas Guérin ◽  
Maxim Dolgushev ◽  
Olivier Bénichou ◽  
Raphaël Voituriez
Keyword(s):  
Reaction Time ◽  
Transport Process ◽  
Reaction Times ◽  
Complex Environments ◽  
Analytical Treatment ◽  
Volume Limit ◽  
Normal Diffusion ◽  
The Mean ◽  
Kinetics Of ◽  
Mean Reaction Time

AbstractChemical reactions generically require that particles come into contact. In practice, reaction is often imperfect and can necessitate multiple random encounters between reactants. In confined geometries, despite notable recent advances, there is to date no general analytical treatment of such imperfect transport-limited reaction kinetics. Here, we determine the kinetics of imperfect reactions in confining domains for any diffusive or anomalously diffusive Markovian transport process, and for different models of imperfect reactivity. We show that the full distribution of reaction times is obtained in the large confining volume limit from the knowledge of the mean reaction time only, which we determine explicitly. This distribution for imperfect reactions is found to be identical to that of perfect reactions upon an appropriate rescaling of parameters, which highlights the robustness of our results. Strikingly, this holds true even in the regime of low reactivity where the mean reaction time is independent of the transport process, and can lead to large fluctuations of the reaction time - even in simple reaction schemes. We illustrate our results for normal diffusion in domains of generic shape, and for anomalous diffusion in complex environments, where our predictions are confirmed by numerical simulations.

Investigations on the Emergence of Larvae from the Cysts of the Potato-root Eel worm, Heterodera rostochiensis.

1951 ◽  
Vol 25 (1-2) ◽  
pp. 49-56 ◽  
Author(s):  
D. W. Fenwick
Keyword(s):  
Standard Deviation ◽  
Reaction Time ◽  
Potato Root ◽  
Maximum Information ◽  
Larval Emergence ◽  
Heterodera Rostochiensis ◽  
The Mean ◽  
Mean Reaction Time

Immersion of cysts of Heterodera rostochiensis in potato-root diffusate results in larval emergence which may occupy up to two or three months. In a previous paper (Fenwick, 1950), the author dealt with the form of the hatching curve, and showed that any given curve was controlled by three parameters; the number of larvae finally emerging, the mean reaction time of the hatchable larvae and the standard deviation of that mean reaction time expressed in log-time units. Since it was also shown that each of these parameters was subject to considerable variation, it is obvious that maximum information and accuracy can only be obtained by continuing a hatching test until all larval emergence has ceased and plotting cumulative hatches at selected intervals of tune against log-time; in this way detailed information may be obtained regarding the parameters of the hatching curve.

The effect of frequent ejaculation in the ram on some semen characteristics

1964 ◽  
Vol 15 (6) ◽  
pp. 950 ◽  
Author(s):  
S Salamon
Keyword(s):  
Reaction Time ◽  
Citric Acid ◽  
High Volume ◽  
Volume Density ◽  
Multiple Factor Analysis ◽  
Citric Acid Concentration ◽  
The Mean ◽  
High Concentrations ◽  
Mean Reaction Time ◽  
Multiple Factor

(1) Series of 13 ejaculates per day for 10 successive days were collected from each of five Merino rams. Every third ejaculate was examined for volume, density, and fructose and citric acid concentrations — 250 samples in all. (2) The mean fructose and citric acid concentrations were 596 and 91 mg% respectively. While the mean fructose concentrations differed significantly (P < 0.001) during the successive 10 days of collection, the citric acid concentrations did not. Both seminal constituents declined with ejaculates within days (P < 0.01 ; P< 0.001). There were significant declines in mean volume and density and an increase in mean reaction time with successive days and with collections within days (P < 0.001). Rams differed significantly in all characteristics examined (P < 0.001). (3) Multiple factor analysis revealed that three factors were mainly responsible for variances in the characteristics studied during the 10 days and within days of collection. The variances in characteristics contributed to by sequence of ejaculates in individual rams over the 10 days were: 68–96% for volume, 90–100% for density, 28–98% for reaction time, and practically nil for fructose and citric acid concentrations. There were marked ram differences in contribution of the sequence of ejaculates to the within-day variance in volume (0–90%), density (0–98%), citric acid concentration (0–98%, and reaction time (0–97%). The ejaculates collected had practically no contribution to the within-day variance in fructose concentration. (4) The rams with relatively low sexual response (long mean reaction time) tended to ejaculate semen of relatively high volume and with high concentrations of fructose and citric acid.

AML FAB Subtypes Shows Different Proportion of Leukemia Initiating Cell Which Has Lower Single Cell Dividing Property.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4568-4568
Author(s):  
Hye Ran Kim ◽  
Myung-Geun Shin ◽  
Yester Kim ◽  
Hyeoung Joon Kim ◽  
Duck Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Single Cell ◽  
Single Cells ◽  
Initial Diagnosis ◽  
Plating Efficiency ◽  
Cell Sorter ◽  
Hematopoietic Stem ◽  
The Mean ◽  
Cell Plating

Abstract Abstract 4568 Background Acute myeloid leukemias (AML) are maintained by a subpopulation of leukemia initiating cell that can regenerate themselves as well as give rise to more differentiated and less proliferative cells that constitute the bulk of the disease. Recent studies discovered both the nature of leukemia stem cells and their origin. Immunophenotype of AML stem cell (ASC) is known as CD34+CD38– leukemic cells. Yet, despite their critical importance, much remains to be learned about the frequency, proportion and dividing activity of ASC in a single cell levels. To address these issues, the current study investigated the frequency, proportion according to AML FAB subtypes and single cell plating efficiency of ASC from bone marrow (BM) at the initial diagnosis. Materials and methods The proportion and frequency of ASC were examined on BM samples of twenty AML patients using single cell sorter (BD FACS Aria cell sorter, BD Biosciences, USA). Growth and proliferation capacities of single normal hematopoietic stem cells and LSC were determined using plating efficiency (number of the wells in which more than two cells grew / total number of cells in 96-well plate culture x 100). Individual single cells were cultured in 96-well plates with each well containing 100 uL of serum media with each 100 ng/mL of stem cell factor, Flt-3, thrombopoietin and G-CSF for five days. Results The frequency and proportion of ASC varied according to FAB subtypes. The mean proportion of ASC from AML M2, M4 and M3 showed 25.1±22.5% (mean±SD), 15.1±16.6% and 6.5±3.3%, respectively. The mean proportion of remaining other subtypes was 13.6±16.2%. CD34 percentage in BM at the initial diagnosis closely correlated with the proportion of ASC. ASC proportion was statistically significant different between patient's group with more than 20% of CD34 and those with less than 20% (P=0.003). Single cell plating efficiency of ASC was markedly reduced compared with non-ASC (CD34+CD38+ leukemic cell population) (7.2 ± 4.7% in ASC vs. 12.6 ± 7.6% in non-ASC). Conclusion ASC proportion and frequency in BM samples were very diverse according to FAB subtypes. This study directly proved lower dividing activity of single ASC, and also implicated strategies for single ASC cloning to develop new therapeutics targeting ASC. Disclosures: No relevant conflicts of interest to declare.

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