Selection for crude protein content in Phalaris tuberosa L. I. Response to selection and preliminary studies on correlated responses

1969 ◽  
Vol 20 (4) ◽  
pp. 643 ◽  
Author(s):  
RJ Clements
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Breeding Population ◽  
In Vitro Digestibility ◽  
Seedling Vigour ◽  
Relative Growth ◽  
Relative Growth Rates ◽  
Selection For ◽  
Pasture Plant

In a highly variable breeding population of P. tuberosa, marked responses were obtained to three generations of selection for high and low crude protein concentration (percentage nitrogen x 6.25) in whole tillers at heading. Total response was similar in each direction, and realized heritability estimates were h2 = 0.25 and h2 = 0.20 in the high and low directions respectively. The responses were accompanied by positively correlated changes in in vitro digestibility and in characters commonly used as indicators of nutritive value of herbage. However, there were large negatively correlated changes in seedling vigour, relative growth rates, and other morphological and physiological characters. The implications of the results for pasture plant breeding are discussed.

CHEMICAL COMPOSITION AND IN VITRO DIGESTIBILITY OF RANGE FORAGE PLANTS OF THE STIPA-BOUTELOUA PRAIRIE

1967 ◽  
Vol 47 (2) ◽  
pp. 161-167 ◽  
Author(s):  
S. Smoliak ◽  
L. M. Bezeau
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Calcium Content ◽  
Early Spring ◽  
In Vitro Digestibility ◽  
Native Grasses ◽  
Stages Of Growth ◽  
Artemisia Species ◽  
Shrubby Species

Five native grasses and one sedge of the Stipa-Bouteloua prairie, three introduced grasses collected at five stages of growth, and four shrubby species collected at three stages of growth were analyzed for proximate chemical constituents.The amounts of phosphorus and digestible and crude protein of all species decreased with maturity, while the cellulose increased. Introduced grasses generally contained more crude protein than native grasses. Shrubby species were higher in crude protein and phosphorus than native grasses. No consistent trend was observed in relative total ash and calcium content at progressive stages of development. The estimated nutritive value index was high for all grasses and low for two Artemisia species. The seasonal declines in crude protein and phosphorus suggest that protein and phosphorus supplements are desirable for range cattle during the fall, winter, and early spring.

GENETIC VARIATION FOR FORAGE QUALITY OF ALFALFA STEMS

1987 ◽  
Vol 67 (4) ◽  
pp. 1057-1067 ◽  
Author(s):  
D. R. BUXTON ◽  
G. C. MARTEN ◽  
J. S. HORNSTEIN
Keyword(s):  
Cell Wall ◽  
Genetic Variation ◽  
Medicago Sativa ◽  
Crude Protein ◽  
Nutritive Value ◽  
Growth Traits ◽  
Forage Quality ◽  
In Vitro Digestibility ◽  
Medicago Sativa L

When normally harvested for hay, the basal portion of alfalfa (Medicago sativa L.) stems is low in forage quality. This study was conducted to determine whether genetic variation for in vitro digestible dry matter (IVDDM) and crude protein (CP) concentration of stems of perennial Medicago spp. could be identified and whether morphological and chemical traits were associated with variation in these characteristics of stems. The lower six nodes and internodes of stems and total herbage from 64 Plant Introduction (PI) accessions and five cultivars of field-grown plants were harvested twice when most plants were at the early seed-pod stage of maturity. Several morphological and growth traits were measured, and both stems and total herbage were evaluated for IVDDM and CP concentration. Covariant analysis was used to adjust in IVDDM and CP concentration of stems for differences in plant maturity. Significant variation for IVDDM and CP concentration in stems existed among the cultivars and among the accessions. Some accessions had significantly higher stem IVDDM or CP concentration than control cultivars. The M. sativa accession, PI 440496, had the fourth highest stem IVDDM and third highest stem CP, with concentrations of 374 and 79 g kg−1, respectively. The number of ridges on stems (stem shape) was significantly correlated with stem IVDDM and CP concentration with average correlation coefficients of 0.30 and 0.35, respectively. Cell-wall concentration and lignin concentration per unit cell wall of stems were both negatively correlated with stem IVDDM and CP concentration. Selection for improved nutritive value of stems may have potential for improving the nutritive value of alfalfa herbage.Key words: Alfalfa, forage quality, crude protein, in vitro digestibility, Medicago sativa L.

scholarly journals Nutritional potentials and in vitro estimation of composite cocoa pod husk-based diets for ruminants

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Oluwatosin Bode Omotoso
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
In Vitro Study ◽  
Metabolizable Energy ◽  
Urea Solution ◽  
In Vitro Digestibility ◽  
Cocoa Pod Husk ◽  
Cassava Peel

Abstract Background The use of cocoa pod husk in livestock nutrition is being limited because of its fibrous nature and anti-nutrients, which have detrimental effect(s) on the animals. This study was conducted to evaluate the nutritive value and effects of replacing urea-treated ensiled cocoa pod husk meal (urea-treated CPHM) with cassava peel in a complete concentrate diet (CCD) on in vitro digestibility and methane (CH4) production. Methods Urea-treated CPHM was prepared by soaking the raw milled pod in 5% urea solution under anaerobic condition for 7 days, and decanted and the filtrates were further ensiled for 28 days, dried, and were replaced with cassava peel meal in CCD at 0, 5, 7.5, 10, 12.5, and 15% on part basis. Feed samples (n = 3) were analyzed for chemical composition, and data generated from in vitro study were subjected to statistical analysis in a completely randomized design experiment. Results Results revealed that the treatment significantly reduced the crude fiber, fiber fraction contents, and anti-nutrients and improved the crude protein content of the pod by 71.84%. Dry matter and crude protein of the diets ranged from 89.34–89.71% and 10.52–12.84%, respectively. The in vitro dry matter digestibility (IVDMD) increased with increasing levels of urea-treated CPHM in the diets. With increasing levels of urea-treated CPHM, CH4 production relatively reduced as compared to diet E. Metabolizable energy (5.66 Kcal/g) of diet F was the highest. Therefore, the inclusion of urea-treated CPHM at 15% in CCDs has the potential for improving digestibility and reducing CH4 production in ruminants.

scholarly journals Evaluasi Nilai Nutrisi dan Kecernaan In Vitro Pelepah Kelapa Sawit (Oil Palm Fronds) yang Difermentasi Menggunakan Aspergillus niger dengan Penambahan Sumber Karbohidrat yang Berbeda

2015 ◽  
Vol 15 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Sitti Wajizah ◽  
Samadi Samadi ◽  
Yunasri Usman ◽  
Elmy Mariana
Keyword(s):  
Aspergillus Niger ◽  
Oil Palm ◽  
Crude Protein ◽  
Nutritive Value ◽  
Crude Fiber ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Oil Palm Fronds ◽  
Palm Fronds

(The evaluation of  nutritive value and In Vitro digestibility of oil palm fronds through fermentation by using  Aspergillus niger  with  different soluble carbohydrate sources) ABSTRACT. Oil palm frond (OPF) is one of potential sources of alternative feed, but has limited use due to high crude fiber and low crude protein contents. Fermentation is one of the methods widely applied to improve nutritive value of animal feed. The purpose of this research is to increase nutritive value of fermented oil palm fronds by adding different soluble carbohydrate source into fermentation media. The results of the experiments indicated that fermented oil palm fronds by Aspergillus niger had a significant effect (P0,05)  on the content of crude protein, crude fiber, and ash. Generally, fermented oil palm fronds with different soluble carbohydrate was able to increase the content of crude protein of oil palm fronds, but not optimal yet in reducing the crude fiber content of fermented substrate. However, the addition of rice bran on the fermentation medium showed the best results, characterized by increasing crude protein and decreasing crude fiber contents as well as improved dry matter and organic matter digestibility, reflected in high concentration of VFA. 

Selection for crude protein content in Phalaris tuberosa L. III. Physiological relationships between crude protein content and seedling vigour.

1973 ◽  
Vol 24 (1) ◽  
pp. 47 ◽  
Author(s):  
RJ Clements
Keyword(s):  
Nitrogen Content ◽  
Protein Content ◽  
Seed Weight ◽  
Crude Protein ◽  
Flag Leaf ◽  
Leaf Node ◽  
Crude Protein Content ◽  
Seedling Vigour ◽  
Relative Growth Rates ◽  
Selection For

Seedling growth of half-sib families from two lines of P. tuberosa previously selected for high and low herbage nitrogen contents respectively was examined in a glasshouse during the autumn and winter. Seedlings from the high nitrogen line were smaller than those of the low nitrogen line but had similar whole-plant relative growth rates. Differences in the rate of nitrogen absorption by the roots (h), and in the rate of seedling weight increase per unit of absorbed nitrogen (EN), accounted for most of the variation in herbage nitrogen content between families. The heights of seedlings were closely correlated with the weights of seeds from which they originated and with EN, and when these two characters were held constant the partial correlation between nitrogen content and seedling weight was negligible (r = -0.05). Among 10 clones differing markedly in herbage nitrogen content in the field, seed weight was closely correlated with the photosynthetic area above the flag leaf node available to each seed, particularly that contributed by the spikelet (r = 0.82***). Two alternative interpretations of this result are discussed. Relationships between nitrogen content, spikelet number, and the areas of photosynthesizing organs above the flag leaf node suggest that seed weight could be maintained during selection for increased nitrogen content by concurrently selecting either for large seeds, or for large flag leaves and a reduced number of spikelets per head. Even so, some loss of actual or potentially attainable seedling vigour seems almost inevitable because of the dependence of nitrogen content on IN and EN and because EN is an important determinant of vigour.

EFFECTS OF 25 YEARS OF N, P AND K FERTILIZATION ON YIELD, PERSISTENCE AND NUTRITIVE VALUE OF A TIMOTHY SWARD

1989 ◽  
Vol 69 (2) ◽  
pp. 501-512 ◽  
Author(s):  
G. BÉLANGER ◽  
J. E. RICHARDS ◽  
R. B. WALTON
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Maximum Yield ◽  
Phleum Pratense ◽  
In Vitro Digestibility ◽  
Botanical Composition ◽  
Phleum Pratense L ◽  
K Fertilization

The effects of over 25 yr of levels of applied N, P and K on forage DM yield, crude protein content, in vitro digestibility (IVD), botanical composition and persistence of a timothy (Phleum pratense L.) sward were assessed in a long-term field experiment on an acidic Riverbank L. Ammonium nitrate (0, 90, 180, 270 kg N ha−1), superphosphate (0, 15, 29, 44 kg P ha−1) and muriate of potash (0, 47, 94, 141 kg K ha−1) were applied annually in all possible combinations to a timothy sward since 1960. In the first 3 yr of the experiment, only N and K were required for maximum yield and persistence of timothy. The requirement for applied P, however, increased with time; in the last 3 yr (1985–1987) of the trial, the highest level of applied P resulted in profitable forage DM yield increases. After 25 yr, the productivity of the timothy sward was dependent upon a balanced application of all three macronutrients; absence of any one nutrient resulted in little or no response to applications of the other nutrients. The long-term persistence of timothy was solely dependent on K fertilization. The proportion of bentgrass and bluegrass species declined with increasing levels of applied K while that of timothy increased. The change in botanical composition due to applied N and K significantly affected IVD and crude protein content.Key words: N, P, K, fertilization, yield, persistence, timothy

scholarly journals Extraction of leaf protein from green crops. Chemical composition and nutritive value of products of fractionation 

1983 ◽  
Vol 55 (2) ◽  
pp. 143-154
Author(s):  
Matti Näsi
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Soluble Sugars ◽  
Protein Solubility ◽  
Leaf Protein ◽  
In Vitro Digestibility ◽  
Growth Stages ◽  
Crude Fibre ◽  
True Protein

Leaf protein was extracted from different green crops in 11 pilot plant esperiments. Of the crops, 4 were grass, 6 clover and one pea. The extraction of juice was on average 55 % of the fresh weight of the green crop and the values for dry matter (DM) and crude protein (CP) were 22.6 and 24.1 %. Clover gave better recoveries of protein than grass. In the leaf protein concentrate (LPC) obtained from the juice, the separation ratios for DM, CP and TP (true protein) were, respectively, 23.7 %, 48.0 and 80.7 %. Heating to 85°C gave more efficient recoveries of LPC than the combination of heating and acid precipitation. The average DM content of the pressed pulp was 30.4 %, the corresponding value for the whole crop being 16.5 %. Measured on a DM basis, the CP content of the pressed pulp was only 0.4 % units lower than in untreated forage, but the crude fibre content was 7.3 % units higher. In vitro organic matter digestibility and the pepsin-HCI solubility of crude protein were on average 5.1 and 5.5 % units lower in the pulp. The average DM of the plant juice was 6.5 % and contained 21.9 % ash, 21.5 % CP, 10.7 % TP and 29.9 % soluble sugars. Clover and pea had much higher values for CP and TP than grass. In the LPC preparations, CP and TP averaged 43.6 % and 38.5 % of DM. Heat treatment gave higher protein content than precipitation of LPC by combined heating and acidification. The in vitro digestibility and protein solubility of LPC were high, on average 85.6 % and 80.2 %. LPC had fairly high contents of lysine and methionine, 4.1 % and 1.6 g/16 g N. There were only small differences in the amino acid composition between grass and clover and between crops harvested at different growth stages. Green crop fractionation is a potential means of improving grassland production and utilization. Promising results have been obtained with plant juice and LPC fed to monogastric animals and pressed pulp residues in diets for ruminants. The economic aspects of fractionation remain to be evaluated.

Growth Response of Wheat (Triticum aestivum) Callus to Imazapyr and in Vitro Selection for Resistance

Weed Science ◽  
1992 ◽  
Vol 40 (2) ◽  
pp. 174-179 ◽  
Author(s):  
David C. Heering ◽  
Arron C. Guenzi ◽  
Thomas F. Peeper ◽  
P. L. Claypool
Keyword(s):  
Growth Response ◽  
In Vitro Selection ◽  
Prediction Interval ◽  
Isoleucine Leucine ◽  
Relative Growth ◽  
Relative Growth Rates ◽  
Selection For ◽  
Wheat Callus ◽  
Percent Growth Inhibition

Intact wheat plants and wheat calli responded similarly to varying concentrations of imazapyr. Fifty percent growth inhibition of wheat callus occurred with 0.05 μM imazapyr after 70 d. As imazapyr concentration increased from 0 to 10 μM, the free isoleucine, leucine, and valine decreased from 160 to 35, 260 to 49, and 310 to 59 pmol mg−1, respectively. Resistant calli, which had relative growth rates exceeding a calculated upper prediction interval, were obtained by in vitro selection at 2 and 5 μM imazapyr. Resistant calli growing on 2 μM imazapyr had free isoleucine, leucine, and valine concentrations intermediate to the control and susceptible callus.

The effects of site and season on the yield and nutritive value of cultivars and half-sib families of perennial ryegrass (Lolium perenne)

2004 ◽  
Vol 44 (8) ◽  
pp. 763 ◽  
Author(s):  
K. F. Smith ◽  
R. J. Simpson ◽  
R. N. Oram
Keyword(s):  
Perennial Ryegrass ◽  
Crude Protein ◽  
Nutritive Value ◽  
Forage Quality ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Water Soluble Carbohydrates ◽  
Sib Families

The effects of site and season on the nutritive value of 16 perennial ryegrass cultivars and 60 half-sib families were assessed at 2 locations in South West Victoria. Crude protein, water-soluble carbohydrates, neutral detergent fibre and in vitro digestibility were measured on vegetative herbage, harvested in either autumn or spring. While no heritable genetic variation for nutritive value parameters was detected in this set of families, consistent differences in the nutritive value of cultivars were measured across sites and seasons. The cultivars Yatsyn1 and Ellett were consistently high in both water-soluble carbohydrates and in vitro digestibility. The differences in mean nutritive value between high and low ranking cultivars were ~40 g/kg water-soluble carbohydrates and 3–5% in vitro digestibility. These consistent differences in forage quality demonstrate the value of measuring forage quality during cultivar evaluation. The identification of cultivars with improved nutritive value will also facilitate the crossing of the alleles that confer this improvement into other genetic backgrounds.

scholarly journals Leaf protein production from energy willow leaves

1983 ◽  
Vol 55 (2) ◽  
pp. 155-162
Author(s):  
Matti Näsi
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Protein Extraction ◽  
Leaf Protein ◽  
In Vitro Digestibility ◽  
Leaf Protein Concentrate ◽  
The Mean ◽  
True Protein ◽  
Leaf Juice

The extraction of protein from leaves of two energy willow clones (Salic cv. Aquatica and S. viminalis) and the chemical composition and nutritive value of the leaf fractions were investigated in six experiments. The preservation of leaf juice and wet leaf protein concentrate (LPC) was also studied. The extraction of juice averaged 33 % of the fresh weight, and the extraction ratios of dry matter (DM) and crude protein (CP) were 10.5 % and 2.9 %. The percentages of juice DM, CP and TP (true protein) separated in LPC were 6 %, 21 % and 48 %, respectively. The separation values were higher when LPC was precipitated by heating than by acidification. Removal of nutrients from the leaves was low and there were only minor differences in composition between the pressed pulp and the leaves. The leaf juice contained 8.3 % DM, of which 20.9 % was ash, 4.9 % CP, 1.9 % TP and 43.2 % sugars. The LPC had an average DM content of 19.6 %, CP being 21.4 % and TP 19.5 % of DM. The in vitro digestibility and pepsin-HCI-solubility of the protein were low. The mean tannin content of the LPC averaged 3.6 % of DM. The mean lysine and methionine contents of the LPC were low, 3.3 and 1.5 g/16 g N, respectively. The preservation of the leaf juice was satisfactory even without addition of preservative. Preservation of wet LPC was good with additives used at the 1.0 % v/w level. Leaf protein extraction was low and the nutritive value of LPC was not particularly high. This may have been partly due to the late date of harvesting and to the fact that the leaves were frozen for storage.

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